A5071685336- Immunotherapy and Immune Responses
- HIV Research and Treatment
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- T-cell and B-cell Immunology
- Reproductive tract infections research
- NF-κB Signaling Pathways
- Cytomegalovirus and herpesvirus research
- HIV/AIDS Research and Interventions
- SARS-CoV-2 and COVID-19 Research
- CAR-T cell therapy research
- Inflammatory Bowel Disease
- RNA Interference and Gene Delivery
- HIV/AIDS drug development and treatment
- Cancer Immunotherapy and Biomarkers
- Cytokine Signaling Pathways and Interactions
- Immune responses and vaccinations
- Viral-associated cancers and disorders
- Neonatal Respiratory Health Research
- Virus-based gene therapy research
- Syphilis Diagnosis and Treatment
- Reproductive System and Pregnancy
- Immune cells in cancer
Ragon Institute of MGH, MIT and Harvard
2013-2019
London School of Hygiene & Tropical Medicine
2016-2019
University of London
2017
Castleton University
2017
Boston Children's Hospital
2011-2014
Harvard University
2011-2014
Beth Israel Deaconess Hospital
2014
Leibniz Institute of Virology (LIV)
2014
Massachusetts Institute of Technology
2014
Massachusetts General Hospital
2014
The cause of Crohn's disease (CD) remains poorly understood. Counterintuitively, these patients possess an impaired acute inflammatory response, which could result in delayed clearance bacteria penetrating the lining bowel and predispose to granuloma formation chronicity. We tested this hypothesis human subjects by monitoring responses killed Escherichia coli injected subcutaneously into forearm. Accumulation 111In-labeled neutrophils at sites 32P-labeled from them were markedly CD. Locally...
Increased IFN-α production contributes to the pathogenesis of infectious and autoimmune diseases. Plasmacytoid dendritic cells (pDCs) from females produce more upon TLR7 stimulation than pDCs males, yet mechanisms underlying this difference remain unclear. In article, we show that basal levels IFN regulatory factor (IRF) 5 in were significantly higher compared with males positively correlated percentage IFN-α-secreting pDCs. Delivery recombinant IRF5 protein into human primary increased...
Several case reports have suggested an association of primary mediastinal germ cell tumor (PMGCT) and Klinefelter's syndrome (KS). In effort to confirm this association, 22 patients with tumors had chromosome studies performed in a prospective fashion. Five (22%) karyotypic or pathologic evidence KS. All the KS nonseminomatous subtype were relatively young (median age, 15 years). The literature confirms findings median age (18 years), subtype, location neoplasm. We conclude that are...
Bruton's tyrosine kinase (Btk), the gene mutated in human immunodeficiency X-linked agammaglobulinemia, is activated by LPS and required for LPS-induced TNF production. In this study, we have investigated role of Btk both signaling via another TLR (TLR2) production other proinflammatory cytokines such as IL-1beta, IL-6, IL-8. Our data show that agammaglobulinemia PBMCs, stimulation with TLR4 (LPS) or TLR2 (N-palmitoyl-S-[2, 3-bis(palmitoyloxy)-(2R)-propyl]-(R)-cysteine) ligands produces...
Abstract SARS-CoV-2 has resulted in high levels of morbidity and mortality world-wide, severe complications can occur older populations. Humoral immunity induced by authorized vaccines wanes within 6 months, frequent boosts may only offer transient protection. GRT-R910 is an investigational self-amplifying mRNA (samRNA)-based vaccine delivering full-length Spike selected conserved non-Spike T cell epitopes. This study reports interim analyses for a phase I open-label dose-escalation trial...
Background Newborns display distinct immune responses that contribute to susceptibility infection and reduced vaccine responses. Toll-like receptor (TLR) agonists may serve as adjuvants, when given individually or in combination, but of neonatal leukocytes many TLR are diminished. TLR8 more effective than other activating human vitro, little is known about whether different distinctly activate leukocytes. We characterized the vitro immuno-stimulatory activities a novel benzazepine agonist,...
ABSTRACT Detection and clearance of bacterial infection require balanced effector resolution signals to avoid chronic inflammation. GNB LPS by TLR4 on mϕ induces inflammatory responses, contributing inflammation tissue injury. LXs Rvs are endogenous lipid mediators that enhance inflammation, their actions primary human responses toward largely uncharacterized. Here, we report LXA4, LXB4, RvD1, tested at 0.1–1 μM, inhibited LPS-induced TNF production from mϕ, with ATL 17(R)-RvD1,...
Systemic chemotherapies for various malignancies have been shown to significantly, yet transiently, decrease numbers of CD4+ T lymphocytes, a major reservoir human immunodeficiency virus type 1 (HIV-1) infection. However, little is known about the impact cytoreductive chemotherapy on HIV-1 dynamics, persistence, and immune responses.We investigated changes in peripheral T-cell-associated DNA RNA levels, lymphocyte activation, viral population structure, virus-specific responses longitudinal...
Even when given 24 hours after lethal radiation, a fragment of an endotoxin-neutralizing protein plus fluoroquinolone antibiotic improves survival and hematopoietic recovery in mice.
Abstract Semi‐interpenetrating polymer networks (semi‐IPNs) containing poly(ethylene glycol)‐diacrylate (PEGdA) and modified gelatin were prepared with 2,2‐dimethoxy‐2‐phenylacetophenone (DMPA) as a photoinitiator. The effect of (i) initiator PEGdA concentration, (ii) weight ratio type on the conversion functional end groups was monitored in situ using attenuated total reflectance‐Fourier transform infrared (ATR‐FTIR). Reaction induction time dependent DMPA concentration increased decreasing...
This study assessed cellular and soluble markers of immune activation in HIV-1 seronegative MSM. MSM profiles were characterized by an increased expression CD57 on T cells endotoxemia. Endotoxin presence was linked to recent high-risk exposure associated with elevated cytokine levels decreased CD4+/CD8+ cell ratios. Taken together, these data show inflammation periods endotoxemia resulting a significantly different phenotype subset at high risk acquisition.
NKG2C is an activating receptor that preferentially expressed on natural killer (NK) cells. The gene encoding (killer cell lectin-like C2, KLRC2) present at different copy numbers in the genomes of individuals. Deletion locus was investigated a case–control study 1522 individuals indigenous to East- and West-Africa association with ocular Chlamydia trachomatis infection its sequelae explored. frequency homozygous KLRC2 deletion 13.7 % Gambians 4.7 Tanzanians. A significantly higher allele...
Background Several non-chlamydial microbial pathogens are associated with clinical signs of active trachoma in trachoma-endemic communities a low prevalence ocular Chlamydia trachomatis (Ct) infection. In the Solomon Islands, Ct among children is despite being moderate. We therefore set out to investigate whether was common infection or dominant polymicrobial community dysbiosis Islands. Methods studied DNA from conjunctival swabs collected 257 Islanders and matched controls. Droplet digital...
Background Trachoma, a neglected tropical disease, is the leading infectious cause of blindness and visual impairment worldwide. Host responses to ocular chlamydial infection resulting in chronic inflammation expansion non-chlamydial bacteria are hypothesised risk factors for development active trachoma conjunctival scarring Methods Ocular swabs from endemic populations The Gambia were selected archived samples 16S sequencing host gene expression. We recruited children with adults scarring,...
Background Crohn's Disease (CD) is a chronic relapsing disorder characterized by granulomatous inflammation of the gastrointestinal tract. Although its pathogenesis complex, we have recently shown that CD patients systemic defect in macrophage function, which results defective clearance bacteria from inflammatory sites. Methodology/Principal Findings Here identified number additional defects following diacylglycerol (DAG) homolog phorbol-12-myristate-13-acetate (PMA) activation. We provide...
BPI (bactericidal/permeability-increasing protein) is a 55 kDa anti-infective molecule expressed in neutrophil and eosinophil granules on some epithelial cells. BPI's high affinity for the lipid A region of endotoxin targets its opsonizing, microbicidal endotoxin-neutralizing activities towards Gram-negative bacteria. Several immunocompromised patient populations demonstrate deficiency, including newborns, those with anti-neutrophil cytoplasmic antibodies (as cystic fibrosis HIV infection)...
Progressive HIV-1 infection leads to both profound immune suppression and pathologic inflammation in the majority of infected individuals. While adaptive dysfunction, as evidenced by CD4+ T cell depletion exhaustion, has been extensively studied, less is known about functional capacity innate populations context infection. Given broad susceptibility opportunistic infections dysregulated observed progressive disease, we hypothesized that there would be significant changes cellular responses....