A5011813995- Composting and Vermicomposting Techniques
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacteriophages and microbial interactions
- Biofuel production and bioconversion
- Wastewater Treatment and Nitrogen Removal
- Microplastics and Plastic Pollution
- Enzyme Production and Characterization
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- SARS-CoV-2 detection and testing
- Ammonia Synthesis and Nitrogen Reduction
- Recycling and Waste Management Techniques
- Microbial Fuel Cells and Bioremediation
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Polysaccharides and Plant Cell Walls
- Evolution and Genetic Dynamics
- Studies on Chitinases and Chitosanases
- Polyamine Metabolism and Applications
- biodegradable polymer synthesis and properties
- Constructed Wetlands for Wastewater Treatment
- CRISPR and Genetic Engineering
- Pesticide and Herbicide Environmental Studies
- Microbial Community Ecology and Physiology
- Carbon and Quantum Dots Applications
Fujian Agriculture and Forestry University
2017-2024
Key Laboratory of Guangdong Province
2023
Nanjing University of Science and Technology
2019
Nanjing Agricultural University
2012-2015
Chinese Academy of Sciences
2015
Institute of Urban Environment
2015
Composting is an efficient way to convert organic waste into fertilizers. However, materials often contain large amounts of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) that can reduce the efficacy treatments when transmitted humans. Because conventional composting fails remove these compounds, we evaluated if hyperthermophilic with elevated temperature more at removing ARGs MGEs explored underlying mechanisms ARG removal two methods. We found removed efficiently...
Abstract Herbicides are one of the most widely used chemicals in agriculture. While they known to be harmful nontarget organisms, effects herbicides on composition and functioning soil microbial communities remain unclear. Here we show that application three herbicides—glyphosate, glufosinate, dicamba—increase prevalence antibiotic resistance genes (ARGs) mobile genetic elements (MGEs) microbiomes without clear changes abundance, diversity bacterial communities. Mechanistically, these...
Abstract While decomposition of organic matter by bacteria plays a major role in nutrient cycling terrestrial ecosystems, the significance viruses remains poorly understood. Here we combined metagenomics and metatranscriptomics with temporal sampling to study mesophilic thermophilic their on during industrial-scale hyperthermophilic composting (HTC). Our results show that virus-bacteria density dynamics activity are tightly coupled, where specific track host densities, triggering microbial...
The horizontal transfer of plasmids has been recognized as one the key drivers for worldwide spread antimicrobial resistance (AMR) across bacterial pathogens. However, knowledge remain limited about contribution made by environmental stress on evolution AMR modulating acquisition and other mobile genetic elements. Here we combined experimental evolution, whole genome sequencing, reverse engineering, transcriptomics to examine if chromosomal triclosan (TCS) disinfectant correlated effects...
Insufficient removal of antibiotics and antibiotic resistance genes (ARGs) from waste products can increase the risk selection for in non-clinical environments. While composting is an efficient way to reduce ARGs, most conventional methods are ineffective at processing highly contaminated fermentation waste. Here we explored efficacy underlying mechanisms hyperthermophilic removing tylosin residues (TFR) associated ARGs mobile genetic elements (MGEs; plasmids, integrons transposon)....
The global spread of plasmid-borne carbapenem resistance is an ongoing public health challenge; however, the nature such horizontal gene transfer events among complex bacterial communities remains poorly understood. We examined in-situ globally dominant New Delhi metallo-β-lactamase (NDM)-5-positive IncX3 plasmid (denoted pX3_NDM-5) in hospital wastewater to simulate a real-world, One Health antimicrobial context.
The rhizosheath, or the layer of soil closely adhering to roots, can help plants tolerate drought under moderate drying conditions. Rhizosheath formation is result poorly understood interactions between root exudates, microbes, and Here, we study roles played by microbiota in rhizosheath barley (a dry crop). We show that greater acid than alkaline soil, inoculation with from enhances soil. rhizosheath-promoting activity associated presence Flavobacteriaceae Paenibacillaceae bacteria express...
Denitrification is an essential step of the nitrogen cycle in soil. However, although sunlight important environmental factor for soil, investigation influence on soil denitrification limited to plant photosynthesis-mediated processes. Herein, a new pathway, denoted as biophotoelectrochemical process, which induced by direct photoexcitation was found greatly enhance denitrification. Using red research object, with irradiation showed nitrate reduction that 2.6-4.7 times faster than without...
Abstract A multiple xylanase system with high levels of activity produced from Penicillium oxalicum GZ-2 using agricultural waste as a substrate has been previously reported. However, the eco-physiological properties and origin multiplicity xylanases remain unclear. In present study, eight active bands were detected zymography all identified putative MALDI-TOF-MS/MS. These are encoded by six different genes. To evaluate functions genes, xyn10A , xyn11A xyn10B xyn11B expressed in Pichia...
Bacterial wilt caused by Ralstonia solanacearum (RS) is a serious threat for agricultural production. In this study, Bacillus amyloliquefaciens strains CM-2 and T-5 antagonistic to RS were used create bioorganic fertilisers control tomato under greenhouse conditions. The possible mechanism of resistance inducement the bacteria was also evaluated.The application significantly reduced incidences (by 63-74%), promoted plant growth populations in rhizosphere compared with control. Both applied...
Agricultural residue is more efficient than purified cellulose at inducing lignocellulolytic enzyme production in Penicillium oxalicum GZ-2, but Trichoderma reesei RUT-C30, induces a response. To understand the reasons, we designed an artificially simulated plant biomass (cellulose plus xylan) to study roles and relationships of each component enzymes by P. GZ-2.The changes activity, gene expression involving (hemi)cellulolytic enzymes, secretome cultures grown on Avicel (A), xylan (X), or...
Endo-1,4-β-mannanase is an enzyme that can catalyze the random hydrolysis of β-1, 4-mannosidic linkages in main chain mannans, glucomannans and galactomannans has a number applications different biotechnology industries. Penicillium oxalicum powerful hemicellulase-producing fungus (Bioresour Technol 123:117-124, 2012); however, few previous studies have focused on cloning expression endo-1,4-β-mannanase gene from oxalicum. A encoding acidophilic thermostable (E.C. 3.2.1.78) GZ-2, which...