A5047842306- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Gut microbiota and health
- Evolution and Genetic Dynamics
- CRISPR and Genetic Engineering
- Legume Nitrogen Fixing Symbiosis
- Insect symbiosis and bacterial influences
- Antimicrobial Resistance in Staphylococcus
- Microbial Metabolic Engineering and Bioproduction
- Plant-Microbe Interactions and Immunity
- Slime Mold and Myxomycetes Research
- Molecular Biology Techniques and Applications
- Evolutionary Game Theory and Cooperation
- Escherichia coli research studies
- Microbial Fuel Cells and Bioremediation
- Plant Pathogenic Bacteria Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Recycling and Waste Management Techniques
- Genomics and Phylogenetic Studies
- Bone Metabolism and Diseases
University of Copenhagen
2015-2024
Aarhus University
2022-2023
University of Washington
2023
Institute for Systems Biology
2023
InSysBio (Russia)
2023
University of Alaska Anchorage
2021
Lillebaelt Hospital
2021
Zero to Three
2016
Columbia University
2015
University of Louisville
2004
In the Origin of Species, Charles R. Darwin [Darwin C (1859) On Species] proposed that struggle for existence must be most intense among closely related species by means their functional similarity. It has been hypothesized this similarity, which results in resource competition, is driver evolution antagonism bacteria. Consequently, should mostly prevalent phylogenetically and metabolically similar species. We tested hypothesis screening all possible pairwise interactions between 67...
Wastewater treatment plants (WWTPs) have been suggested as reservoirs and sources of antibiotic resistance genes (ARGs) in the environment. In a WWTP ecosystem, human enteric environmental bacteria are mixed exposed to pharmaceutical residues, potentially favoring genetic exchange thus ARG transmission. However, contribution microbial communities WWTPs dissemination remains poorly understood. Here, we examined for first time plasmid permissiveness an activated sludge community by utilizing...
Summary Social interactions in which bacteria respond to one another by modifying their phenotype are central determinants of microbial communities. It is known that interspecific influence the biofilm bacteria; a fitness bacteria. However, underlying role fundamental ecological factors, specifically coexistence and phylogenetic history, formation remains unclear. This study examines how social affect multi‐species co‐cultures from five diverse environments. We found prevalence increased...
Abstract Many prokaryotes encode CRISPR-Cas systems as immune protection against mobile genetic elements (MGEs), yet a number of MGEs also harbor components. With few exceptions, loci encoded on are uncharted and comprehensive analysis their distribution, prevalence, diversity, function is lacking. Here, we systematically investigated across the largest curated collection natural bacterial archaeal plasmids. widely but heterogeneously distributed plasmids and, in comparison to host...
Many bacteria use CRISPR-Cas systems to combat mobile genetic elements, such as bacteriophages and plasmids
The global spread of plasmid-borne carbapenem resistance is an ongoing public health challenge; however, the nature such horizontal gene transfer events among complex bacterial communities remains poorly understood. We examined in-situ globally dominant New Delhi metallo-β-lactamase (NDM)-5-positive IncX3 plasmid (denoted pX3_NDM-5) in hospital wastewater to simulate a real-world, One Health antimicrobial context.
As biofilms grow, resident cells inevitably face the challenge of resource limitation. In opportunistic pathogen Pseudomonas aeruginosa PA14, electron acceptor availability affects matrix production and, as a result, biofilm morphogenesis. The secreted polysaccharide Pel is required for pellicle formation and colony wrinkling, two activities that promote access to O2. We examined exploitability evolvability at air-liquid interface (during formation) on solid surfaces formation). Although...
Microorganisms frequently co-exist in matrix-embedded multispecies biofilms. Within biofilms, interspecies interactions influence the spatial organization of member species, which likely play an important role shaping development, structure and function these communities. Here, a reproducible four-species biofilm, composed Stenotrophomonas rhizophila, Xanthomonas retroflexus, Microbacterium oxydans Paenibacillus amylolyticus, was established to study importance individual species during...
Abstract The rise of β-lactam resistance among pathogenic bacteria, due to the horizontal transfer plasmid-encoded β-lactamases, is a current global health crisis. Importantly, hydrolyzation by not only protects producing cells but also sensitive neighboring cooperatively. Yet, how such cooperative traits affect plasmid transmission and maintenance currently poorly understood. Here we experimentally show that KPC-2 β-lactamase expression extracellular activity were higher when encoded on...
Community assembly processes determine patterns of species distribution and abundance which are central to the ecology microbiomes. When studying plant root microbiome assembly, it is typical sample at whole system scale. However, sampling these relatively large spatial scales may hinder observability intermediate processes. To study relative importance processes, we employed millimetre-scale cell elongation zone individual roots. Both rhizosphere rhizoplane microbiomes were examined in...
Abstract Bacillus subtilis is a soil bacterium that competent for natural transformation. Genetically distinct B. swarms form boundary upon encounter, resulting in killing of one the strains. This process mediated by fast-evolving kin discrimination (KD) system consisting cellular attack and defence mechanisms. Here, we show these swarm antagonisms promote transformation-mediated horizontal gene transfer between strains low relatedness. Gene interacting non-kin largely unidirectional, from...
Plasmids facilitate rapid bacterial adaptation by shuttling a wide variety of beneficial traits across microbial communities. However, under non-selective conditions, maintaining plasmid can be costly to the host cell. Nonetheless, plasmids are ubiquitous in nature where bacteria adopt their dominant mode life - biofilms. Here, we demonstrate that biofilms act as spatiotemporal reserves for plasmids, allowing them persist even conditions. these spatial stratification plasmid-carrying cells...
Abstract Horizontal gene transfer via plasmids is important for the dissemination of antibiotic resistance genes among medically relevant pathogens. Specifically, IncHI1A believed to facilitate spread genes, such as carbapenemases, within clinically family Enterobacteriaceae. The microbial community urban wastewater treatment plants has been shown be highly permissive towards conjugal IncP1 plasmids. Here, we tracked P1 plasmid pB10 and HI1A R27 in communities present residential sewage...
Although organic matter may accumulate sometimes (e.g. lignocellulose in peat bog), most natural biodegradation processes are completed until full mineralization. Such transformations often achieved by the concerted action of communities interacting microbes, involving different species each performing specific tasks. These interactions can give rise to novel "community-intrinsic" properties, through e.g. activation so-called "silent genetic pathways" or synergistic interplay between...
The gut is a hot spot for transfer of antibiotic resistance genes from ingested exogenous bacteria to the indigenous microbiota. objective this study was determine fate two nearly identical blaCMY-2-harboring plasmids introduced into human fecal microbiota by Escherichia coli strains isolated and poultry meat. chromosome CMY-2-encoding plasmid both were labeled with distinct fluorescent markers (mCherry green protein [GFP]), allowing fluorescence-activated cell sorting (FACS)-based tracking...