A5020499721- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Celiac Disease Research and Management
- Probiotics and Fermented Foods
- Microscopic Colitis
- Infant Nutrition and Health
- Polyamine Metabolism and Applications
- Drug Transport and Resistance Mechanisms
- Galectins and Cancer Biology
- Barrier Structure and Function Studies
- Animal testing and alternatives
- Helicobacter pylori-related gastroenterology studies
- Viral gastroenteritis research and epidemiology
- Oral health in cancer treatment
- Pharmacological Effects and Assays
- Pancreatic and Hepatic Oncology Research
- Neutropenia and Cancer Infections
- Veterinary medicine and infectious diseases
- Gastroesophageal reflux and treatments
- Blood properties and coagulation
- Infant Health and Development
- Neuroendocrine Tumor Research Advances
- Spectroscopy Techniques in Biomedical and Chemical Research
- Ultrasound and Hyperthermia Applications
- Cancer survivorship and care
Karolinska Institutet
2017-2024
Karolinska University Hospital
2024
University of Helsinki
2003-2019
University of Turku
2006-2017
Albert Einstein College of Medicine
2015
Chemotherapy-induced gastrointestinal toxicity (CIGT) is a complex process that involves multiple pathophysiological mechanisms. We have previously shown commonly used chemotherapeutics 5-fluorouracil, oxaliplatin, and irinotecan damage the intestinal mucosa increase permeability to iohexol. hypothesized CIGT associated with alterations in fecal microbiota metabolome. Our aim was characterize these changes examine how they relate severity of CIGT. A total 48 male Sprague–Dawley rats were...
Infant microbiota is influenced by numerous factors, such as delivery mode, environment, prematurity and diet (breast milk or formula). In addition to its nutritional value, breast contains bioactive substances that drive microbial colonisation support immune system development, which are usually not present in infant formulas. Among these substances, polyamines have been described be essential for intestinal functions newborns. However, their effect on the establishment of remains unclear....
Antibiotics that are excreted into the intestinal tract promote antibiotic resistance by exerting selective pressure on gut microbiota. Using a beagle dog model, we show an orally administered targeted recombinant beta-lactamase enzyme eliminates portion of parenteral ampicillin is small intestine, preventing ampicillin-induced changes to fecal microbiota without affecting levels in serum. In dogs receiving ampicillin, significant disruption and emergence ampicillin-resistant Escherichia...
Gastrointestinal toxicity is the most common adverse effect of chemotherapy. Chemotherapeutic drugs damage intestinal mucosa and increase permeability. Intestinal permeability one key markers gastrointestinal function measuring could serve as a useful tool for assessing severity chemotherapy-induced toxicity.Male Sprague-Dawley rats were injected intraperitoneally either with 5-fluorouracil (150 mg/kg), oxaliplatin (15 mg/kg) or irinotecan (200 mg/kg). Clinical signs assessed daily by...
A characteristic feature of celiac disease is the presence circulating autoantibodies targeted against transglutaminase 2 (TG2), reputed to have a function in angiogenesis. In this study we investigated whether TG2-specific derived from patients inhibit angiogenesis both ex vivo and models sought clarify mechanism behind phenomenon. We used murine aorta-ring mouse matrigel-plug assays address aforementioned issues. found be impaired as result antibody supplementation systems. Our results...
Housing conditions are essential for ensuring animal welfare and high-quality research outcomes. In this study, we continuously monitored air quality-specifically ammonia, carbon dioxide, relative humidity, temperature-in Individually Ventilated Cages (IVCs) housing five female or male C57BL/6N mice. The cages were cleaned either weekly bi-weekly, the data collected as mice aged from 100 to 348 days. survival rate remained above 96%, with body weight increasing by 35-52% during study period....
Seven beagles in a colony of dogs had chronic diarrhea for at least 30 days. The were subsequently treated with tylosin 20 mg/kg BW q24h PO 10 During the treatment period, feces became firmer but remained loose. When was discontinued, reappeared 3 weeks. abnormally loose all metronidazole, trimethoprim-sulfadiazine, or doxycycline and prednisone. diet then changed days from highly digestible moist pet food to dry developed normal adult dogs. again firmer, although still some period extended...
Demonstrated competence in laboratory animal science (LAS) is a prerequisite Directive 2010/63/EU to work with animals used scientific procedures, as it essential increase welfare, improve the quality of science, promote acceptability research and meet demands free movement personnel exchange. Although since 2010 there have been eight clear steps achieving required working not uncommon see documentation for individuals who just completed an LAS course that contains only education training...
The search for existing non-animal alternative methods use in experiments is currently challenging because of the lack both comprehensive structured databases and balanced keyword-based strategies to mine unstructured textual databases. In this paper we describe 3Ranker, which a fast, keyword-independent algorithm finding biomedical research. 3Ranker was created by using machine learning approach, consisting Random Forest model built on dataset 35 million abstracts constructed with weak...
Seven beagles in a colony of dogs had chronic diarrhea for at least 30 days. The were subsequently treated with tylosin 20 mg/kg BW q24h PO 10 During the treatment period, feces became firmer but remained loose. When was discontinued, reappeared 3 weeks. abnormally loose all metronidazole, trimethoprim-sulfadiazine, or doxycycline and prednisone. diet then changed days from highly digestible moist pet food to dry developed normal adult dogs. again firmer, although still some period extended...
Previous studies using a BALB/cOlaHsd model have shown the impact that supplementation of infant formula with polyamines has on modulation microbial colonization and immune system development.
The 51Cr-EDTA test is a valuable clinical tool for screening intestinal diseases in dogs. performed by calculating the percentage of recovery from urine PO-ingested dose after 6 or 24 hours. Careful collection practical limitation this dogs, and our goal was to develop simpler that measures blood. A absorption simultaneously on serum 43 times healthy Beagle Dogs. Timed blood samples were withdrawn, collected during 6-hour period. Percentages ingested then calculated serum. mean +/- standard...