A5071173421- Adipokines, Inflammation, and Metabolic Diseases
- Kruppel-like factors research
- Cytokine Signaling Pathways and Interactions
- Folate and B Vitamins Research
- Cancer, Hypoxia, and Metabolism
- Alzheimer's disease research and treatments
- Nitric Oxide and Endothelin Effects
- Aquaculture disease management and microbiota
- DNA Repair Mechanisms
- Retinoids in leukemia and cellular processes
- Adenosine and Purinergic Signaling
- Cholinesterase and Neurodegenerative Diseases
- Genomics, phytochemicals, and oxidative stress
- Neuroscience of respiration and sleep
- Click Chemistry and Applications
- TGF-β signaling in diseases
- MicroRNA in disease regulation
- Carbohydrate Chemistry and Synthesis
- Protein Hydrolysis and Bioactive Peptides
- Biomarkers in Disease Mechanisms
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Neuroinflammation and Neurodegeneration Mechanisms
- Genetic factors in colorectal cancer
- Aquaculture Nutrition and Growth
- Vitamin D Research Studies
University of Tasmania
2009-2023
Agency for Science, Technology and Research
2008-2023
Kaohsiung Medical University
2013-2023
Menzies School of Health Research
2023
Institute of Molecular and Cell Biology
2009-2023
Kaohsiung Medical University Chung-Ho Memorial Hospital
2013-2021
National Yang Ming Chiao Tung University
1999-2005
University System of Taiwan
2005
Institute of Biomedical Sciences, Academia Sinica
2004
Taichung Veterans General Hospital
1999
Visfatin is an adipocytokine involved in cellular metabolism, inflammation, and cancer. This study investigated the roles of extracellular visfatin breast cancer, explored underlying mechanisms clinical experimental settings.Associations serum with clinicopathologic characteristics patient survival were assessed Cox regression models Kaplan-Meier analyses. Effects on cultured cancer cells examined, followed by vivo investigation tumor growth metastasis xenograft animal models. Imatinib...
Visfatin, an adipocytokine highly expressed in breast tumor tissues, is associated with cancer progression. Recent studies showed that adipocytokines mediate development through tumor-stromal interactions the microenvironment. This study focused on interaction between one key stromal constituent—tumor-associated macrophages—and visfatin. Pretreatment of THP-1 and peripheral blood mononuclear cells (PBMCs) recombinant visfatin resulted M2-polarization determined by CD163 CD206 expression....
Abstract Background Interleukin-1 receptor antagonist (IL-1RA), a member of the IL-1 family, has diverse roles in cancer development. However, role IL-1RA oral squamous cell carcinoma (OSCC), particular underlying mechanisms, remains to be elucidated. Methods Tumor tissues from OSCC patients were assessed for protein expression by immunohistochemistry. Patient survival was evaluated Kaplan–Meier curve analysis. Impact differential on cultured lines vitro clonogenic survival, tumorsphere...
Abstract: This study characterizes and examines the P2 receptor‐mediated signal transduction pathway of a rat brain‐derived type 2 astrocyte cell line, RBA‐2. ATP induced Ca 2+ influx activated phospholipase D (PLD). The ATP‐stimulated was inhibited by pretreating cells with receptor antagonist, pyridoxal‐phosphate‐6‐azophenyl‐2′,4′‐disulfonic acid (PPADS), in concentration‐dependent manner. agonist 2′‐ 3′‐ O ‐(4‐benzoylbenzoyl)adenosine 5′‐triphosphate (BzATP) stimulated largest increases...
The amyloid precursor protein (APP) is well studied for its role in Alzheimer disease. However, little known about normal function. In this study, we examined the of APP neural stem/progenitor cell (NSPC) proliferation. NSPCs derived from APP-overexpressing Tg2576 transgenic mice proliferated more rapidly than corresponding background strain (C57Bl/6xSJL) wild-type mice. contrast, knock-out (APP-KO) had reduced proliferation rates when compared with (C57Bl/6). A secreted factor, identified...
In the present study, we used N terminus (amino acids 1 approximately 160) of type VI adenylyl cyclase (ACVI) as bait to screen a mouse brain cDNA library and identified Snapin novel ACVI-interacting molecule. is binding protein SNAP25, component SNARE complex. Co-immunoprecipitation analyses confirmed interaction between full-length ACVI. Mutational analysis revealed that domains ACVI were located within amino 86 33-51 Snapin, respectively. Co-localization was observed in primary...
Background Alzheimer's disease (AD) is caused by accumulation of Aβ, which produced through sequential cleavage β-amyloid precursor protein (APP) the β-site APP cleaving enzyme (BACE1) and γ-secretase. Enoxaparin, a low molecular weight form glycosaminoglycan (GAG) heparin, has been reported to lower Aβ plaque deposition improve cognitive function in AD transgenic mice. Methodology/Principal Findings We examined whether heparin enoxaparin influence processing inhibit production primary...
Abstract The tumor microenvironment represents one of the main obstacles in breast cancer treatment owing to presence heterogeneous stromal cells, such as adipose-derived stem cells (ADSCs), that may interact with and promote development. Resistin is an adipocytokine associated adverse progression; however, its underlying mechanisms context remain largely unidentified. Here, we utilized a transwell co-culture model containing patient-derived ADSCs cell lines investigate their potential...
// Chun-Hao Tsai 1, 2, * , Amos C. Hung Yuan-Yin Chen 1 Ya-Wen Chiu Pei-Wen Hsieh 3 Yi-Chen Lee 4 Yu-Han Su Po-Chih Chang 5 Stephen Chu-Sung Hu 6, 7 Shyng-Shiou F. Yuan 8 Translational Research Center, Kaohsiung Medical University Hospital, University, Kaohsiung, Taiwan 2 Graduate Institute of Medicine, College Natural Products, School Traditional Chinese and Biomedical Sciences, Gung Taoyuan, Department Anatomy, Division General Surgery, E-Da Hospital/I-Shou 6 Dermatology, Obstetrics...
// Yu-Jen Cheng 1,2 , Yi-Chen Lee 3 Wen-Chin Chiu 4 Jen-Wei Tsai 5 Yu-Han Su 6 Amos C. Hung Po-Chih Chang 7 Chih-Jen Huang 8 Chee-Yin Chai 9 and Shyng-Shiou F. Yuan 6,10 1 Division of Thoracic Surgery, Department Cancer Center, E-DA Hospital, Kaohsiung, Taiwan 2 Postgraduate Medicine, School Medicine for International Student, I-Shou University, Respiratory Therapy, College Kaohsiung Medical University Anatomic Pathology, Translational Research Research, Obstetrics Gynecology, General...
ATP has been shown to mediate stress responses in the brain. The present study examined ATP-stimulated protein expression of RBA-2 type-2 astrocytes. Our results revealed that stimulated HSP60 a dose- and time-dependent manner. stimulation requires minimal concentration 500 microM high extracellular (1 mM) significant increase from 2 24 h. In addition, expressions were inhibited by inhibitors for kinase C (PKC) phospholipase D (PLD), antioxidants, resveratrol, catalase. Furthermore, Cu/Zn...
Alzheimer's disease is associated with abnormal accumulation of Aβ, which produced from the β-amyloid precursor protein (APP) by β-site APP-cleaving enzyme (BACE1) and γ-secretase. Our previous studies showed that heparin can decrease APP processing decreasing levels BACE1 ADAM10. In this study, we examined effects glycosaminoglycans (GAGs) on Aβ production aim understanding specificity effects. Various GAG analogs were incubated primary cortical cells derived (SW)Tg2576 mice level APP,...
Abstract We investigated p53-dependent gene expression in nitric oxide (NO)-induced apoptosis of two tumor cell types. Seventy-seven putative p53-regulated genes were screened for NO-mediated changes. Twenty-four up-regulated and three down-regulated significantly by NO human neuroblastoma cells. Genes known to be involved apoptosis, which ≥2-fold, included FAS, CASP-1, BIK, PUMA, DR4 the serpins maspin (SERPINB5), plasminogen activator inhibitor-1 (PAI-1). Real-time PCR confirmed PAI-1...
Exposure to arsenic is known be a risk factor for various types of cancer. Apart from its carcinogenic activity, also shows promoting effects on angiogenesis, crucial process tumor growth. Yet, the mechanism underlying arsenic-induced angiogenesis not fully understood. In this study, we aimed at investigating involvement inhibitor DNA binding 1 (Id1) and associated signal molecules in arsenic-mediated angiogenesis. Our initial screening revealed that treatment with low concentrations (0.5-1...
The stimulus-transcriptional coupling during ischemia/hypoxia was examined for ATP-stimulated expression of immediate early genes (IEGs; c-fos, zif268, c-myc and nur77) in a rat brain-derived type 2 astrocyte cell line, RBA-2. Incubation cells with 1 mM extracellular ATP stimulated time-dependent c-fos zif268. induced the largest increases zif268 mRNA lesser one mRNA. also slight increase nur77 but ineffective inducing these cells. Brief exposure to potassium cyanide simulate chemical...