A5012677805- Cystic Fibrosis Research Advances
- Glycosylation and Glycoproteins Research
- Neonatal Respiratory Health Research
- Tracheal and airway disorders
- Probiotics and Fermented Foods
- Invertebrate Immune Response Mechanisms
- Gut microbiota and health
- Advanced Chemical Sensor Technologies
- Proteoglycans and glycosaminoglycans research
- Carbohydrate Chemistry and Synthesis
- Micro and Nano Robotics
- Photoacoustic and Ultrasonic Imaging
- Optical Coherence Tomography Applications
- Bacterial biofilms and quorum sensing
- Pickering emulsions and particle stabilization
- Virus-based gene therapy research
- Antimicrobial Peptides and Activities
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Animal Virus Infections Studies
- Respiratory viral infections research
- Inhalation and Respiratory Drug Delivery
- Ultrasound Imaging and Elastography
- MicroRNA in disease regulation
- Acoustic Wave Resonator Technologies
- Bacteriophages and microbial interactions
University of North Carolina at Chapel Hill
2010-2024
Lung Institute
2016-2024
University of Washington
1991
Airway epithelium structure/function can be altered by local inflammatory/immune signals, and this process is called epithelial remodeling. The mechanism which innate response regulated, causes mucin/mucus overproduction, largely unknown. Exosomes are nanovesicles that secreted internalized cells to transport cellular cargo, such as proteins, lipids, miRNA. objective of study was understand the role exosomes play in airway remodeling through cell–cell communication. We used two different...
Chronic lung diseases such as cystic fibrosis, chronic bronchitis, and asthma are characterized by hypersecretion poor clearance of mucus, which associated with prognosis mortality. Little is known about the relationship between biophysical properties mucus its molecular composition. The mucins MUC5B MUC5AC traditionally believed to generate characteristic airway mucus. However, contribution hundreds globular proteins not clear. Approximately one-third total proteome comprises distinct,...
Significance Gel-forming mucins, MUC5AC and MUC5B, are the essential components of protective mucus layer lungs. MUC5B is crucial for lung innate defense, generally secreted disproportionately in response to cigarette smoke allergens. MUC5AC’s role airway defense chronic disease pathogenesis how this emerges from its macromolecular assembly/gel-making properties not known. We demonstrate binds significantly more hydrophobic surfaces forms stiffer viscoelastic layers compared MUC5B. Also,...
The airway milieu of individuals with muco-obstructive diseases (MADs) is defined by the accumulation dehydrated mucus due to hyperabsorption surface liquid and defective mucociliary clearance. Pathological becomes progressively more viscous age disease severity concentration overproduction mucin host-derived extracellular DNA (eDNA). Respiratory MADs provides a niche for recurrent persistent colonization respiratory pathogens, including
Directed manipulation of nanomaterials has significant implications in the field nanorobotics, nanobiotechnology, microfluidics and directed assembly. With goal highly controllable nanomaterial mind, we present a technique for near-surface manoeuvering magnetic nanorod swimmers its application to controlled micromanipulation. We fabricate Janus nanorods show that rotation these near floor results predictable translational motion. The plane is nearly parallel floor, angle between rod tilt...
In cystic fibrosis (CF) lungs, epithelial Na+ channel (ENaC) hyperactivity causes a reduction in airway surface liquid volume, leading to decreased mucocilliary clearance, chronic bacterial infection, and lung damage. Inhibition of ENaC is an attractive therapeutic option. However, antagonists have failed clinically because off-target effects the kidney. The S18 peptide naturally occurring short palate nasal clone 1 (SPLUNC1)-derived antagonist that restores height for up 24 h CF human...
Understanding the basic elements of airway mucosal surfaces and how they form a functional barrier is essential in understanding disease initiation, progression, pathogenesis ultimately treating chronic lung diseases. Using primary epithelial cell cultures, atomic force microscopy (AFM), multiangle light scattering quartz crystal micro balance with dissipation monitoring techniques, here we report that membrane bound mucins (MBMs) found periciliary layer (PCL) surface are densely decorated...
Mucociliary clearance (MCC) plays an essential role in maintaining airway sterility and health. Conversely, mucociliary dysfunction is implicated across many obstructive diseases. Understanding the necessary requirements for successful MCC imperative to establish pathology of disease, as well develop therapeutic strategies. Although postural, that is, gravitational, drainage used clinically aid mucus clearance, it ignored both animal cell culture models MCC. In this study, we a novel assay...
Cystic fibrosis (CF) is a genetic defect in the cystic transmembrane conductance regulator protein and most common life-limiting condition affecting Caucasian population. It an autosomal recessive, monogenic inherited disorder characterized by failure of airway host defense against bacterial infection, which results bronchiectasis, breakdown wall extracellular matrix (ECM). In this study, we show that vitro models consisting human tracheo-bronchial-epithelial (hBE) cells grown on porous...
Smoking remains a leading cause of preventable morbidity and mortality worldwide. Despite downward trend in cigarette use, less-regulated tobacco products, such as cigarillos, which are often flavored to appeal specific demographics, younger people, becoming increasingly popular. Cigar/cigarillo smoking has been considered safer alternative cigarettes; however, the health risks associated with cigar comparison not well understood. To address this knowledge gap, we characterized effects...
Abstract The airway milieu of individuals with muco-obstructive diseases (MADs) is defined by the accumulation dehydrated mucus due to hyperabsorption surface liquid and defective mucociliary clearance. Pathological becomes progressively more viscous age disease severity concentration overproduction mucin host-derived extracellular DNA (eDNA). Respiratory MADs provides a niche for recurrent persistent colonization respiratory pathogens, including Pseudomonas aeruginosa , which responsible...