A5016808498- Autophagy in Disease and Therapy
- Cancer-related Molecular Pathways
- Plant-derived Lignans Synthesis and Bioactivity
- Microtubule and mitosis dynamics
- Cell death mechanisms and regulation
- Curcumin's Biomedical Applications
- Cancer, Lipids, and Metabolism
- NF-κB Signaling Pathways
- Natural product bioactivities and synthesis
- Bioactive Compounds and Antitumor Agents
- Genomics, phytochemicals, and oxidative stress
- Ferroptosis and cancer prognosis
- Sirtuins and Resveratrol in Medicine
- Histone Deacetylase Inhibitors Research
- Cancer, Hypoxia, and Metabolism
- Calcium signaling and nucleotide metabolism
- Epigenetics and DNA Methylation
- Synthesis of Indole Derivatives
- Metabolism and Genetic Disorders
- Synthesis and bioactivity of alkaloids
- Ginseng Biological Effects and Applications
- Animal Nutrition and Physiology
- Cancer-related molecular mechanisms research
- Drug-Induced Hepatotoxicity and Protection
- Endoplasmic Reticulum Stress and Disease
National Taiwan Normal University
2015-2025
Cornell University
1994-2016
New York State College of Veterinary Medicine
2016
Chang Jung Christian University
2005-2012
National Cheng Kung University
2008
Kaohsiung Medical University
2008
National Institutes of Health
2003
National Institute of Diabetes and Digestive and Kidney Diseases
2003
National Yang Ming Chiao Tung University
2002-2003
Thioridazine (THD) is a common phenothiazine antipsychotic drug reported to suppress growth in several types of cancer cells. We previously showed that THD acts as an antiglioblastoma and anticancer stem-like cell agent. However, the signaling pathway underlying autophagy apoptosis induction remains unclear. treatment significantly induced with upregulated AMPK activity engendered death increased sub-G1 glioblastoma multiform (GBM) lines. Notably, through whole gene expression screening...
Ferroptosis, a recently discovered form of iron-dependent cell death, requires an increased level lipid-reactive oxygen species (ROS). Ferritinophagy, ferritin degradation pathway, depends on selective autophagic cargo receptor (NCOA4). By screening various types natural compounds, formosanin C (FC) was identified as novel ferroptosis inducer, characterized by attenuations FC-induced viability inhibition and lipid ROS formation in the presence inhibitor. FC also induced flux, evidenced...
Dysfunction of degradation machineries causes cancers, including hepatocellular carcinoma (HCC). Overexpression cyclin D1 in HCC has been reported. We previously reported that autophagy preferentially recruits and degrades the oncogenic microRNA (miR)‐224 to prevent HCC. Therefore, present study, we attempted clarify whether is another factor selectively regulated by tumorigenesis. Initially, found an inverse correlation between low autophagic activity high expression tumors 147 patients...
Lipolysis in adipocytes governs the release of fatty acids for supply energy to various tissues body. This reaction is mediated by hormone-sensitive lipase (HSL), a cytosolic enzyme, and perilipin, which coats lipid droplet surface adipocytes. Both HSL perilipin are substrates polyphosphorylation protein kinase A (PKA), phosphorylation required induce translocate from cytosol droplet, critical step lipolytic (Sztalryd C., Xu, G., Dorward, H., Tansey, J. T., Contreras, J.A, Kimmel, A. R.,...
Curcumin, an active compound in turmeric and curry, has been proven to induce tumor apoptosis inhibit proliferation, invasion, angiogenesis, metastasis via modulating numerous targets various types of cancer cells. Aurora A is a mitosis-related serine-threonine kinase plays important roles diverse human cancers. However, the effect curcumin on not reported. In this study, promoter activity mRNA expression were inhibited curcumin-treated bladder T24 cells, suggesting that regulated at...
Targeting ferritin via autophagy (ferritinophagy) to induce ferroptosis, an iron- and reactive oxygen species (ROS)-dependent cell death, provides novel strategies for cancer therapy. Using a ferroptosis-specific inhibitor iron chelator, the vulnerability of triple-negative breast (TNBC) MDA-MB-231 cells ferroptosis was identified compared that luminal A MCF-7 cells. Saponin formosanin C (FC) revealed as potent inducer characterized by superior induction in cytosolic lipid ROS formation well...
The E. coli strains harboring the polyketide synthase (pks) island encode genotoxin colibactin, a secondary metabolite reported to have severe implications for human health and progression of colorectal cancer. present study involves whole-genome-wide comparison phylogenetic analysis pks isolates gain insight into distribution evolution these organisms. Fifteen isolated from patients with ulcerative colitis (UC) were sequenced, 13 which harbored islands. In addition, 2,654 genomes public...
The natural product justicidin A, an arylnaphthalide lignan isolated from Justicia procumbens, significantly inhibited the growth of human colorectal cancer cells HT-29 and HCT 116 at day 6 post-treatment. Further study revealed that A-treated died apoptosis. Justicidin A treatment caused DNA fragmentation increase in phosphatidylserine exposure cells. number sub-G1 phase was also increased upon treatment. Caspase-9 but not caspase-8 activated, suggesting damaged mitochondria. mitochondrial...
Our previous reports showed that justicidin A (JA), a novel and pure arylnaphthalide lignan isolated from Justicia procumbens , induces apoptosis of human colorectal cancer cells hepatocellular carcinoma cells, leading to the suppression both tumor cell growth in NOD‐SCID mice. Here, we reveal JA autophagy HT‐29 by conversion autophagic marker LC3‐I LC3‐II. Furthermore, LC3 puncta vesicle formation, SQSTM1/p62 were observed. Administration inhibitor (bafilomycin A1 chloroquine) transfection...
Abstract Formosanin C (FC) is a natural compound extracted from Paris formosana Hayata with anticancer activity. FC induces both autophagy and apoptosis in human lung cancer cells. FC‐induced depolarization of mitochondrial membrane potential (MMP) may trigger mitophagy. In this study, we clarified the effect on autophagy, mitophagy, role FC‐related cell death motility. We found caused continuous increase LC3 II (representing autophagosomes) 24 to 72 h without degradation after treatment...
Three experiments were conducted to determine the effect of excess dietary protein on threonine requirement broiler chicks 14 d age (Experiments 1 and 2) from 16 28 (Experiment 3). Two levels used in Experiments 2:20% CP a threonine-limiting basal diet containing wheat, peanut meal, selected amino acids 25% same supplemented with mixture lacking threonine. A based corn, soybean was also included Experiment 2. The determined using single level (20%) 3. Threonine requirements estimated by...
Formosanin C is a pure compound isolated from Paris formosana Hayata (Liliaceae). The antitumor efficacy of formosanin has been observed in cultured cells and animal systems. However, the molecular mechanisms remain unknown. results present study indicate that induced apoptosis HT-29 characterized by exposure phosphatidylserine, accumulation at sub-G(1) phase, fragmentation DNA, change nuclear morphology time- dose-related manner. apoptotic signaling cascades may proceed via proteolytic...
Justicia procumbens is a traditional Taiwanese herbal remedy used to treat fever, pain, and cancer. Justicidin A, isolated from , has been reported suppress in vitro growth of several tumor cell lines as well hepatoma cells. In this study, justicidin A activated caspase‐8 increase tBid, disrupted mitochondrial membrane potential (Δ ψ m ), caused the release cytochrome c Smac/DIABLO Hep 3B G2 also reduced Bcl‐x L increased Bax Bak mitochondria. Caspase‐8 inhibitor (Z‐IETD) attenuated...
Drug resistance and tumor recurrence are major obstacles in treating lung cancer patients. Accumulating evidence considers stem cells (CSCs) as the contributor to these clinical challenges. Agents that can target CSCs could potentially provide a more effective treatment than traditional chemotherapy. Here, we utilized side-population (SP) method isolate from A549 PC-9 cell lines. Subsequently, high throughput platform, connectivity maps (CMAPs), was used identify potential anti-CSC agents....
Curcumin, an active natural compound in turmeric and curry, has been reported to exhibit anti-cancer effect. Cisplatin, carboplatin oxaliplatin are used treat various types of cancers. However, acquired resistance toxicities observed. Here, the addition curcumin significantly increased cytotoxicity drugs on human colorectal cancer HT-29 cells, producing synergistic (cisplatin carboplatin) additivity (oxaliplatin) effects. Treatments combination with resulted a induction apoptosis occurrence...
Analysis of various public databases revealed that HRAS gene mutation frequency and mRNA expression are higher in bladder urothelial carcinoma. Further analysis the roles oncogenic HRAS, autophagy, cell senescence signaling cancer cells sensitized to anticancer drug cisplatin using phytochemical pterostilbene. A T24 line with was chosen for further experiments. Indeed, coadministration pterostilbene increased stronger cytotoxicity on compared wild-type E7 cells, which paralleled by neither...
Abstract Background Despite advances in treatment, patients with refractory colorectal cancer (CRC) still have poor long-term survival, so there is a need for more effective therapeutic options. Methods To evaluate the HDAC8 inhibition efficacy as CRC we examined effects of various inhibitors (HDAC8i), including BMX (NBM-T-L-BMX-OS01) combination temozolomide (TMZ) or other standard drugs on p53 mutated HT29 cells, well wild-type HCT116 and RKO cells. Results We showed that HDAC8i TMZ...
Bufotalin is one of the bufadienolides isolated from Formosan Ch'an Su, which made skin and parotid glands toads. Ingestion toad venom results in severe morbidity high mortality. Although Su clinically toxic, it has been used as an important traditional Chinese medicine for heart failure pains. In this study, bufotalin-induced apoptosis human hepatocellular carcinoma Hep 3B cells was investigated. The indicate that externalization phosphatidylserine, accumulation sub-G1 cells, fragmentation...
Coronavirus disease 2019 (COVID-19) remains a threat with the emergence of new variants, especially Delta and Omicron, without specific effective therapeutic drugs. The infection causes dysregulation immune system cytokine storm that eventually leads to fatal acute respiratory distress syndrome (ARDS) further irreversible pulmonary fibrosis. Therefore, promising way inhibit is disrupt binding fusion between viral spike host ACE2 receptor. A transcriptome-based drug screening platform has...