A5025270564- Advanced Breast Cancer Therapies
- Cancer-related Molecular Pathways
- Neuroinflammation and Neurodegeneration Mechanisms
- Pancreatic and Hepatic Oncology Research
- Graphene and Nanomaterials Applications
- bioluminescence and chemiluminescence research
- Lymphoma Diagnosis and Treatment
- Advanced biosensing and bioanalysis techniques
- Cancer, Hypoxia, and Metabolism
- Viral-associated cancers and disorders
- Diet and metabolism studies
- Telomeres, Telomerase, and Senescence
- Immune Cell Function and Interaction
- Genomics and Chromatin Dynamics
- Cardiac Ischemia and Reperfusion
- Metabolism and Genetic Disorders
- Metabolism, Diabetes, and Cancer
- Ion channel regulation and function
- Retinoids in leukemia and cellular processes
- Histone Deacetylase Inhibitors Research
- RNA modifications and cancer
- Chronic Lymphocytic Leukemia Research
- Protease and Inhibitor Mechanisms
- Epigenetics and DNA Methylation
- Amino Acid Enzymes and Metabolism
Samsung Medical Center
2012-2024
Sungkyunkwan University
2013-2024
Samsung (South Korea)
2019-2024
Biomedical Research Institute
2008-2012
Eunice Kennedy Shriver National Institute of Child Health and Human Development
1996
National Institutes of Health
1996
A phase I dose-escalation clinical trial of peritumoral injections interleukin 12 (IL-12)-transduced autologous fibroblasts was performed in patients with disseminated cancer for whom effective treatment does not exist. The goals this study were to assess the safety and toxicities as well efficacy, ancillarily immunomodulatory effects, IL-12 gene transfer. Primary dermal cultured from transduced retroviral vector carrying human genes (p35 p40) neomycin phosphotransferase (TFG-hIL-12-Neo)....
Rituximab is a CD20-targeted monoclonal antibody widely used in the treatment of B-cell lymphoma. Previously, we have shown that Epstein–Barr virus (EBV) latent membrane protein-1 (LMP1) increases chemoresistance malignant cancer cells. In this study examined effects LMP1 on response lymphoma cell lines to rituximab. Here show for first time activates Akt pathway and up-regulates Mcl-1 through miR-155 expression, which contributes survival rituximab-treated Furthermore, inhibition or...
Methods for reproducibly isolating and enriching small extracellular vesicles (EVs) from blood are essential clinical utilization of EVs in cancer patients.We combined ultracentrifugation (UC) with polymer-based precipitation (ExoQuick [EQ] or Total Exosome Isolation [TEI] kit) to isolate (diameter, 30-150 nm) the serum breast compared performance four cycles UC (UC4x) that two followed by enrichment using EQ (UC2x→EQ) TEI (UC2x→TEI) kits.The mean concentration isolated 1 mL UC2x→EQ (139.0 ±...
The phosphoinositol 3-kinase (PI3K) pathway is associated with poor prognosis of hematologic malignancies, providing a strong rationale for the use PI3K inhibitors in treatment malignant lymphoma. However, development resistance limits lymphoma patients.We established copanlisib (pan-PI3K inhibitor)-resistant B-cell and duvelisib (PI3Kδ -γ T-cell cell lines. cytokine array phospho-kinase were used to identify up-regulated proteins resistant cells. Cytokine expression levels examined by ELISA...
Blockage of B cell receptor signaling with ibrutinib presents a promising clinical approach for treatment B-cell malignancies. However, many patients show primary resistance to the drug or develop secondary resistance. In current study, cDNA microarray and Western blot analyses revealed CD79B upregulation in activated cell-like diffuse large lymphoma (ABC-DLBCL) that display differential ibrutinib. overexpression was sufficient induce enhanced AKT MAPK activation, indicative an alternative...
Background: Exosomes have emerged as important mediators of tumor progression, and a prognostic role for serum exosomal miRNAs has been suggested in multiple myeloma (MM).Given the association hypoxia with aggressiveness, including cancer stem cell-like phenotypes, we explored from MM cells under hypoxic conditions analyzed their diverse roles both promoting oncogenic activity predicting prognosis.Methods: The human cell line, RPMI 8226, was cultured exosome production miRNA profiles were...
Epstein–Barr virus (EBV)-encoded latent membrane protein-1 (LMP1) is a transmembrane protein essential for EBV-induced immortalization and transformation of B cells. Activation-induced cytidine deaminase (AID) triggers somatic hypermutation recombination, in turn contributing to lymphomagenesis. Here, we report an intracellular mechanism by which LMP1 contributes cell lymphomagenesis via AID expression. In our experiments, increased mRNA expression promoter activity. The region contains...
Glioblastoma multiforme (GBM) is an extremely aggressive brain cancer with a median survival of less than 2 years. GBM characterized by abnormal activation receptor tyrosine kinase and constitutively activated STAT3. Although EGFR phosphorylation STAT3 are essential for the maintenance stem cells, molecular mechanism underlying endosome-mediated not fully understood. In current study, we showed that GTP-binding protein RRAD (RAS associated diabetes, RAD) physically associates EGFR, EEA1,...
Abstract Maspin inhibits metastasis of some cancer cells, and clinical studies have identified correlations between maspin loss poor prognosis in several types. was found to be significantly overexpressed lung samples as compared with matched normal tissues. However, the regulatory mechanism expression remains unclear. We show here that differential carcinoma-derived cells is regulated at transcriptional level. p63 a critical factor for transcription maspin, which lost highly invasive such...
Senescence limits cellular proliferation, and therefore might be a mechanism which could suppress the progression of cancer. Herein we show that E2F1, transcription factor essential to cell cycle progress main target tumor suppressor Rb, is critical barrier for induction senescence. Human cancer cells transfected with siE2F1 were shown express replicative senescence markers, in addition yielding positive results upon SA-beta-Gal staining. Consistent notion role overexpressed E2F1 proved...
Maspin is a tumor suppressor protein that has been reported to stimulate the cell death of cancer and inhibit metastasis cancer. The present study aimed explore survival pathway by which maspin modulates resistance human lung cells chemotherapeutic drugs, consequences gene therapy in an animal model.NCI-H157 A549 were transfected with either mock vector (pCMVTaq4C), (pCMV-maspin), siControl or siMaspin. RT-PCR Western blot analysis performed expressions proteins cDNA microarray was carried...
Inflammatory biomarkers, such as the neutrophil to lymphocyte ratio (NLR), monocyte (LMR), and Glasgow Prognostic Score (GPS) have been proposed predict prognosis in diffuse large B-cell lymphoma (DLBCL). C-X-C motif ligand 10 (CXCL10) is a chemokine released from inflammatory cells tumor microenvironment known promote cell migration invasion. In this study, we investigated clinical impact of pretreatment serum level CXCL10 on prognostic value biomarkers 313 patients with DLBCL who were...
Epstein-Barr virus (EBV)-encoded nuclear antigen, EBNA2, expressed in EBV-infected B lymphocytes is critical for lymphoblastoid cell growth. Microarray profiling and cytokine array screening revealed that EBNA2 associated with upregulation of the chemokines CCL3 CCL4 lymphoma cells. Depletion or inactivation sensitized DLBCL cells to doxorubicin. Our results indicate EBV influences survival via an autocrine mechanism whereby increases CCL4, which turn activate Btk NF-κB pathways,...
Summary Natural killer/T‐cell lymphoma (NKTL) is a highly aggressive disease. Despite the use of various treatment regimens, prognosis NKTL poor, and new strategies need to be determined. Because significant survival potential, nuclear factor (NF)‐ κ B has become one major targets for drug development. In this study, we explored effect action mechanism NF‐ inhibitors, BAY 11‐7082 curcumin, on cell lines (NKL, NK‐92 HANK1). Electrophoretic mobility shift assay showed that was constitutively...
Senescence is an important determinant of treatment outcome in cancer therapy. In the present study, we show that knockdown inwardly rectifying K(+) channel Kir2.2 induced growth arrest without additional cellular stress cells lacking functional p53, p16, and/or Rb. also senescence-associated β-galactosidase activity and upregulated senescence marker proteins multiple cell lines derived from different tissues, including prostate, stomach, breast. Interestingly, a significant increase...
Ras associated with diabetes (Rad) is a Ras-related GTPase that promotes cell growth by accelerating cycle transitions. Rad knockdown induced arrest and premature senescence without additional cellular stress in multiple cancer lines, indicating expression might be critical for the these cells. To investigate precise function of this process, we used human as bait yeast two-hybrid screening system sought Rad-interacting proteins. We identified Grap2 cyclin D interacting protein...
// Kyung Ju Ryu 1, * , Chaehwa Park 2, Mineui Hong 3 Young Hyeh Ko 4 Won Seog Kim 5 Seok Jin 1 Department of Health Sciences and Technology, Samsung Advanced Institute for Sungkyunkwan University, Seoul, Korea 2 Biomedical Research Institute, Medical Center, Pathology, Kangnam Sacred Heart Hospital, Hallym University School Medicine, Division Hematology Oncology, These authors contributed equally to this work Correspondence to: Kim, email: kstwoh@skku.edu Keywords: stem cell, FOXO4,...
Senescence, an inherent tumor suppressive mechanism, is a critical determinant for chemotherapy. In the present study, we show that monocarboxylate transporter 2 (MCT2) protein was tumor-selectively expressed in human colorectal malignancies and knockdown of MCT2 induces mitochondrial dysfunction, cell-cycle arrest, senescence without additional cellular stress cancer cell lines. Moreover, reactive oxygen species (ROS) scavenger, N-acetylcysteine, blocked knockdown-induced growth arrest...
// Jung Yong Hong 1 , Kyung Ju Ryu 2 Chaehwa Park 3 Mineui 4 Young Hyeh Ko 5 Won Seog Kim 6 Seok Jin 2, Department of Oncology, Asan Medical Center, University Ulsan College Medicine, Seoul, South Korea Health Sciences and Technology, Samsung Advanced Institute for Sungkyunkwan University, Biomedical Research Institute, School Pathology, Kangnam Sacred Heart Hospital, Hallym Division Hematology-Oncology, Correspondence to: Kim, email: kstwoh@skku.edu Keywords: survivin, epstein-barr virus,...
Ku70, a DNA repair protein, was recently identified as critical anti-apoptotic protein that inhibits Bax translocation to mitochondria. The dissociation of from Ku70 is essential for the apoptotic activity Bax. Here, we show maspin, tumor suppressor frequently lost in cancer, regulates this process. Maspin increased cell death acetylation-dependent manner. inhibited histone deacetylase 1 (HDAC1) and thus acetylation which led induction apoptosis. These results reveal maspin Ku70-interacting...