A5089330086- Antimicrobial Peptides and Activities
- Chemical Synthesis and Analysis
- Biochemical and Structural Characterization
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Asymmetric Hydrogenation and Catalysis
- Peptidase Inhibition and Analysis
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Platelet Disorders and Treatments
- Cell Adhesion Molecules Research
- Click Chemistry and Applications
- Crystal structures of chemical compounds
- Protein Hydrolysis and Bioactive Peptides
- Antimicrobial Resistance in Staphylococcus
- Synthesis and biological activity
- Bacterial biofilms and quorum sensing
- Synthesis of β-Lactam Compounds
- Cell death mechanisms and regulation
- Antibiotic Resistance in Bacteria
- Asymmetric Synthesis and Catalysis
- Metal complexes synthesis and properties
- Pediatric health and respiratory diseases
- Antibiotics Pharmacokinetics and Efficacy
- Antimicrobial agents and applications
Royal College of Surgeons in Ireland
2014-2023
University of Medicine and Health Sciences
2022
22q11 Ireland
2015-2017
University College Dublin
2006-2015
University of Wisconsin–Madison
2009
Washington University in St. Louis
2009
Conway School of Landscape Design
2006
J P Kenny
2004
University of Pennsylvania
2001
Villanova University
2001
Ischemic and excitotoxic events within the brain result in rapid often unfavorable depletions neuronal energy levels. Here, we investigated signaling pathways activated response to energetic stress created by transient glutamate excitation cerebellar granule neurons. We characterized a glucose dependent hyperpolarization of mitochondrial membrane potential (Δψ m ) majority neurons after excitation. Expression levels primary transporters (GLUTs) isoforms 1, 3, 4, 8 were found be unaltered 24...
Excitotoxicity after glutamate receptor overactivation induces disturbances in cellular ion gradients, resulting necrosis or apoptosis. Excitotoxic is triggered by rapid, irreversible ATP depletion, whereas the ability to recover bioenergetics suggested be necessary for activation of excitotoxic In this study, we demonstrate that even a transient decrease and an associated adenosine monophosphate–activated protein kinase (AMPK) We show Bcl-2 homology domain 3 (BH3)–only Bim, proapoptotic...
Two novel polyarginine labelled ruthenium polypyridyl dyes are reported, one conjugated to five, (Ru–Ahx–R5), and eight arginine residues, (Ru–Ahx–R8); both complexes exhibit long-lived, intense, oxygen-sensitive luminescence; (Ru–R8) is passively, efficiently very rapidly transported across the cell membrane into cytoplasm without requirement for its permeablisation.
There is increasing evidence that vascular endothelial growth factor (VEGF) has autocrine as well paracrine functions in tumour biology. Vascular factor-mediated cell survival signalling occurs via the classical tyrosine kinase receptors Flt-1, KDR/Flk-1 and more novel neuropilin (NP) receptors, NP-1 NP-2. A 24-mer peptide, which binds to neuropilin-1, induced apoptosis of murine human breast carcinoma cells, whereas a peptide directed against KDR had no effect. Both anti-NP1 anti-KDR...
Here, we demonstrate that antimicrobial peptides (AMPs) are an effective antibiofilm treatment when applied as catheter lock solutions (CLSs) against S. aureus biofilm infections. The activity of synthetic AMPs (Bac8c, HB43, P18, Omiganan, WMR, Ranalexin, and Polyphemusin) was measured early mature biofilms produced by methicillin-resistant methicillin-susceptible isolates from patients with device-related infections grown under in vivo-relevant conditions. cytotoxic hemolytic activities the...
The capacity of ruthenium polypyridyl complexes as probes for combined confocal luminescence and resonance Raman imaging, enabled by their large Stokes shift, is demonstrated a novel membrane sensitive Ru(ii) peptide. Confocal imaging provides complementary information about the phospholipid regions cell location dye within cell.
RNA interference (RNAi) holds great promise as a strategy to further our understanding of gene function in the central nervous system (CNS) and therapeutic approach for neurological neurodegenerative diseases. However, potential its use is hampered by lack siRNA delivery vectors which are both safe highly efficient. Cyclodextrins have been shown be efficient low toxicity various cell types vitro. date, they not exploited oligonucleotides neurons. To this end, modified β-cyclodextrin (CD)...
The targeted delivery of luminescent Ru(II) polypyridyl complexes to the nucleus live mammalian cells by a Nuclear Localisation Signal (NLS) peptide; NF-κB is demonstrated.
X-linked inhibitor of apoptosis protein (XIAP) is a potent caspases 3, 7 and 9, mitochondrial Smac (second mitochondria-derived activator caspase) release during inhibits the activity XIAP. In this study we show that cytosolic XIAP also feeds back to mitochondria impair release. We constructed fluorescent XIAP-fusion by labelling NH2- COOH-termini with Cerulean (C-XIAP-C). Immunoprecipitation confirmed C-XIAP-C retained ability interact impaired extrinsically intrinsically activated in...
Amidophosphine−phosphinite ligands (AMPP) derived from (S)-N-benzylmandelamide ((S)-R,R'-benzylmandelNOP (S)-1 (R = R' phenyl) and (S)-7 phenyl, cyclopentyl)), (S)-N-methylmandelamide ((S)-R,R'-methylmandelNOP (S)-2 (S)-8 (S)-N-methyllactamide ((S)-R,R'-methyllactaNOP (S)-3 (S)-9 (S)-2-(hydroxymethyl)-2-pyrrolidinone ((S)-R,R'-oxoProNOP (S)-4−6 (S)-10 (R, cyclohexyl, cyclopentyl)) have been prepared in high yields (60−94%) reacted with rhodium precursors to prepare neutral "Rh{AMPP}"...
Delivery of macromolecular drugs to airway cells after inhalation can be limited by rapid clearance, in vivo degradation, and poor intracellular targeting. Liposome carriers offer an effective method improving drug stability, but conventional liposomes have targeting capacity are cleared rapidly the lungs. Further modification is required improve liposome-cell interaction Therefore, we proposed conjugating three arginine-rich membrane translocating peptides, namely, HIV-TAT, Antennapedia,...
We introduce CycloPs, software for the generation of virtual libraries constrained peptides including natural and nonnatural commercially available amino acids. The is written in cross-platform Python programming language, features include generating one-dimensional SMILES three-dimensional SDF formats, suitable screening. stand-alone capable filtering using empirical measurements, peptide synthesizability by standard synthesis techniques, stability, druglike properties peptide. accompanying...
Aqueous soluble NIR-AZA fluorochrome which undergoes rapid rt bio-conjugations <italic>via</italic> thiol addition for <italic>in vitro</italic> and vivo</italic> imaging.
OPINION article Front. Immunol., 05 October 2012Sec. Molecular Innate Immunity Volume 3 - 2012 | https://doi.org/10.3389/fimmu.2012.00309
Reported is the synthesis of azo mutual prodrugs nonsteroidal anti-inflammatory agents (NSAIDs) 4-aminophenylacetic acid (4-APAA) or 5-aminosalicylic (5-ASA) with peptides, including an antibiotic peptide temporin analogue modified at amino terminal by α-aminoisobutyric (Aib) residue. These are designed for colonic delivery two to treat infection and inflammation bacterial pathogen Clostridium difficile .