A5101259544- Extracellular vesicles in disease
- Tissue Engineering and Regenerative Medicine
- Cardiovascular Effects of Exercise
- Vitamin D Research Studies
- Health and Medical Research Impacts
- Comparative Literary Analysis and Criticism
- RNA Interference and Gene Delivery
- Latin American Literature Analysis
- Virus-based gene therapy research
- Biomedical and Engineering Education
- Viral Infections and Immunology Research
- Electrospun Nanofibers in Biomedical Applications
- Bone health and osteoporosis research
- Fuel Cells and Related Materials
- Artificial Intelligence in Healthcare and Education
- Ion channel regulation and function
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Cardiac Structural Anomalies and Repair
- MicroRNA in disease regulation
- Cardiac Fibrosis and Remodeling
- Literary and Philosophical Studies
- Cancer Risks and Factors
- Literature, Magical Realism, García Márquez
- Orthopaedic implants and arthroplasty
Imperial College London
2020-2024
Lung Institute
2024
University of Florence
1999
Abstract Aims Altered mechanical load in response to injury is a main driver of myocardial interstitial fibrosis. No current vitro model can precisely modulate multicellular environment while maintaining physiological behaviour. Living slices (LMS) are 300 μm‐thick cardiac preparation with preserved structure and function. Here we apply varying degrees preload rat human LMS evaluate early cellular, molecular, functionality changes related Methods results Left ventricular were obtained from...
Cardiac fibroblasts regulate the development of adult cardiomyocyte phenotype and cardiac remodeling in disease. We investigate role that fibroblasts-secreted extracellular vesicles (EVs) have modulation Ca2+ cycling-a fundamental mechanism function universally altered during EVs collected from cultured human ventricular were purified by centrifugation, ultrafiltration size-exclusion chromatography. The presence EV markers identified dot blot analysis electron microscopy....
Gene therapy based on viral vectors offers great potential for the study and treatment of cardiac diseases. Here we explore use Living Myocardial Slices (LMS) as a platform nucleic acid-based therapies. Rat LMS Adeno-Associated viruses (AAV) were used to optimise analyse gene transfer efficiency, viability, tissue functionality, cell tropism in tissue. Human from failing (dilated cardiomyopathy) hearts was also validate model more translational setting. cultured at physiological sarcomere...
In an ethnically homogeneous population of women living in Tuscany, Italy, the relationships between age, body weight, bone mineral density and vitamin D receptor (VDR) gene polymorphism were studied, with objective recognizing patients at risk for osteoporosis. 275 was measured by Dual Energy X-rays Absorptiometry (DEXA). 50 them individual genetic pattern VDR evaluated DNA extraction followed PCR amplification gene, digestion restriction enzyme BsmI. Age inversely related (R2 = 0.298)....
Abstract BACKGROUND Small extracellular vesicles (sEVs) released in the cardiac microenvironment are reported to regulate remodelling, partially via microRNA transfer. Harvesting sEVs produced exclusively from myocardium remains challenging and a solid research platform for sEV cardiovascular testing needs be established. Organotypic living myocardial slices (LMS) allow mimic disease record electrophysiological responses biological pharmacological stimuli. This study aims at understanding...
Abstract Background The heart is exposed to mechanical forces and continuously adapts its environment in a process known as cardiac remodeling. Excessive load primary driver of pathological remodeling, leading failure (HF), prominent cause morbidity mortality worldwide. A set microRNAs (miRs), termed mechano-miRs, has been identified respond contribute pathophysiology HF. N6-methyladenosine (m6A) RNA modification emerging novel regulatory layer impacting gene expression occurring both coding...
Abstract Lactic acid is recognized as an alternative fuel source for various tissues and acknowledged its protective effects in the brain. However, potential a cardioprotective agent remains controversial. Here, we aimed to (1) evaluate impact of acute L-lactic administration, given at onset reperfusion, on myocardial infarct size isolated mouse hearts submitted transient global ischemia, (2) assess chronic exposure living slices (LMS) from human hearts, (3) elucidate underlying mechanisms....
Background: Microvascular dysfunction is a hallmark of hypertrophic obstructive cardiomyopathy (HOCM). Factors modulating myocardial microcirculation in response to disease phenotype require further studies. This study aims investigate microvascular remodeling HOCM patients using quantitative imaging toolset that combines classical histological screening, ultrastructure assessment, 3D molecular and automated segmentation tissue specimens. Methods: was studied cohort 20 consecutive who...
Engagement of the sarcoplasmic reticulum (SR) Ca2+ stores for excitation–contraction (EC)-coupling is a fundamental feature cardiac muscle cells. Extracellular matrix (ECM) proteins that form extracellular scaffolding supporting contractile activity are thought to play an integral role in modulation EC-coupling. At baseline, human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) show poor utilisation SR stores, leading inefficient EC-coupling, like developing or CMs diseases...
Abstract Introduction Mechanical overload plays an important role in the progression of chronic heart failure (CHF). Beside numerous neurohormonal and chemical stimuli that contribute to structural functional changes myocardium, intercellular communication mediated by extracellular vesicles (EVs), also a pivotal disease progression. Exosomes are subset EVs ranging between 30–150 nm; these mediators carry molecular cargoes such as miRNAs, mRNAs, DNA several proteins. However, it is still...
Abstract Background The inability of the myocardium to regenerate is a determining factor in heart failure (HF).Platforms that model cardiac disease require approximate vivo environment and related factors.One such mechanical load, vitro, application this has been by static, isometric systems mimic preload only.Application both afterload, however, better recapitulates cycle.Mechanical load relevant neonatal tissue, where perinatal period sees changes haemodynamics, loss CM's regenerative...
Abstract Introduction Mechanical load is an important determinant of cardiac output, and excessive mechanical can lead to heart remodelling, ultimately failure. Cardiac intercellular communication partially mediated by extracellular vesicles (EVs), which are 30-200 nm sized nanoparticles acting as shuttles many bioactive cargoes that modulate cell behaviour, such microRNAs. Living myocardial slices ideal platform study how affect through EVs, they be manipulated obtain different degrees...
Abstract Background Gene therapy based on viral vectors is a promising new approach and offers great potential for the study treatment of cardiac diseases. Nucleic acid-based transfection human heart tissue could be considered valid translational alternative to use transgenic animal models in cardiovascular research. Purpose Here we explore Living Myocardial Slices (LMS) as platform nucleic therapies. Rat LMS Adeno-Associated viruses (AAV) were used optimise analyse gene transfer efficiency,...
Abstract Introduction Mechanical load is a determinant of the healthy-to-failing myocardium transition. Extracellular vesicles (EVs) found in range 30–200 nm contain bioactive cargoes such as DNA, miRNA and proteins, thus are considered important mediators paracrine crosstalk between cells health disease. Living myocardial slices (LMS) 300μm layers tissue that allow modelling intercellular communication heart. Purpose To assess whether secretion EVs derived from physiological or pathological...