A5044001723- Tissue Engineering and Regenerative Medicine
- Cardiovascular Function and Risk Factors
- Cardiomyopathy and Myosin Studies
- Electrospun Nanofibers in Biomedical Applications
- Cardiac electrophysiology and arrhythmias
- Cardiac Structural Anomalies and Repair
- Cardiac Fibrosis and Remodeling
- Mechanical Circulatory Support Devices
- 3D Printing in Biomedical Research
- Elasticity and Material Modeling
- Bone Tissue Engineering Materials
- Pluripotent Stem Cells Research
- Neuroscience and Neural Engineering
- Cardiac Ischemia and Reperfusion
- Cardiac Valve Diseases and Treatments
- Fuel Cells and Related Materials
- Cardiovascular Effects of Exercise
- Integrated Circuits and Semiconductor Failure Analysis
- Heart Rate Variability and Autonomic Control
- Cellular Mechanics and Interactions
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
University of Pennsylvania
2023-2024
Imperial College London
2019-2023
Presbyterian Hospital
2023
Hammersmith Hospital
2019-2021
Engineered heart tissue (EHT) strategies, by combining cells within a hydrogel matrix, may be novel therapy for failure. EHTs restore cardiac function in rodent injury models, but more data are needed clinically relevant settings. Accordingly, an upscaled EHT patch (2.5 cm × 1.5 mm) consisting of up to 20 million human induced pluripotent stem cell–derived cardiomyocytes (hPSC-CMs) embedded fibrin-based was developed. A rabbit myocardial infarction model then established test feasibility and...
Cardiac remodelling is the process by which heart adapts to its environment. Mechanical load a major driver of remodelling. tissue culture has been frequently employed for in vitro studies load-induced remodelling; however, current protocols (e.g. cyclic stretch, isometric load, and auxotonic load) are oversimplified do not accurately capture dynamic sequence mechanical conformational changes experienced vivo. This limits translational scope relevance findings.We developed novel methodology...
Abstract Aims Altered mechanical load in response to injury is a main driver of myocardial interstitial fibrosis. No current vitro model can precisely modulate multicellular environment while maintaining physiological behaviour. Living slices (LMS) are 300 μm‐thick cardiac preparation with preserved structure and function. Here we apply varying degrees preload rat human LMS evaluate early cellular, molecular, functionality changes related Methods results Left ventricular were obtained from...
Determining transmural mechanical properties in the heart provides a foundation to understand physiological and pathophysiological cardiac mechanics. Although work on characterisation has begun isolated cells permeabilised samples, profile of living individual layers not been examined. Myocardial slices are 300 μm-thin sections tissue with preserved cellular stoichiometry, extracellular matrix, structural architecture. This allows for mechanics assays context an intact vitro organotypic...
Abstract Mechanical load is one of the main determinants cardiac structure and function. studied in vitro using preparations together with loading protocols (e.g., auxotonic, isometric). However, such studies are often limited by reductionist models poorly simulated mechanical profiles. This hinders physiological relevance findings. Living myocardial slices have been used to study vitro. (LMS) 300‐μm‐thick intact organotypic obtained from explanted animal or human hearts. They preserved...
Vascular and valvular calcifications, commonly seen in renal patients, increase operative mortality can preclude conventional management. We show a novel approach to treat aortic stenosis degenerative mitral regurgitation under hypothermic circulatory arrest hemodialysis patient with aortic, disease porcelain aorta surgical transcatheter contraindications.
Abstract Introduction The importance of transmural heterogeneity for left ventricular (LV) function is well recognised. Mid-wall systolic shortening a better predictor cardiovascular morbidity than ejection fraction. While variation in electrical properties well-documented, mechanical differences remain poorly characterised. Most studies are based on isolated cells or permeabilised preparations, with limited data acquired living multicellular myocardial preparations. Purpose Here, we test...
Abstract Background The inability of the myocardium to regenerate is a determining factor in heart failure (HF).Platforms that model cardiac disease require approximate vivo environment and related factors.One such mechanical load, vitro, application this has been by static, isometric systems mimic preload only.Application both afterload, however, better recapitulates cycle.Mechanical load relevant neonatal tissue, where perinatal period sees changes haemodynamics, loss CM's regenerative...