A5023595490- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Angiogenesis and VEGF in Cancer
- Electrowetting and Microfluidic Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Biosensing Techniques and Applications
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- 3D Printing in Biomedical Research
- Atherosclerosis and Cardiovascular Diseases
- Economic Theory and Policy
- RNA Interference and Gene Delivery
- Advanced Fluorescence Microscopy Techniques
- Glycosylation and Glycoproteins Research
- Viral Infectious Diseases and Gene Expression in Insects
- Sustainable Finance and Green Bonds
- Photoacoustic and Ultrasonic Imaging
- Proteoglycans and glycosaminoglycans research
- CAR-T cell therapy research
- bioluminescence and chemiluminescence research
- Monoclonal and Polyclonal Antibodies Research
- Superconducting Materials and Applications
- International Business and FDI
- Magnetic confinement fusion research
- Spectroscopy Techniques in Biomedical and Chemical Research
Dublin City University
2013-2024
United Kingdom Atomic Energy Authority
2023
Culham Science Centre
2023
Damien Foundation
2018
22q11 Ireland
2014
Unisys (United States)
1998
With the ability to affect multiple genes and fundamental pathways simultaneously, miRNA engineering of Chinese Hamster Ovary (CHO) cells has significant advantages over single gene expression or repression. Tight control these molecular triggers is desirable as it could in theory allow on/off even tunable regulation cellular phenotypes. The present study investigated potential employing a tetracycline inducible (TET-On) system for conditional knockdown specific miRNAs but encountered...
Centrifugal microfluidic systems have become one of the principal platforms for implementing bioanalytical assays, most notably biomedical point-of-care diagnostics. These so-called 'lab-on-a-disc' primarily utilise rotationally controlled centrifugal field in combination with capillary forces to automate a range laboratory unit operations (LUOs) sample preparation, such as metering, aliquoting, mixing and extraction biofluids well sorting, isolation counting bioparticles. LUOs been...
We describe a centrifugal microfluidic 'Lab-on-a-Disc' (LoaD) technology for DNA purification towards eventual integration into Sample-to-Answer platform detection of the pathogen Escherichia coli O157:H7 from food samples. For this application, we use novel architecture which combines 'event-triggered' dissolvable film (DF) valves with reaction chamber gated by centrifugo-pneumatic siphon valve (CPSV). This permits comprehensive flow control simple changes in speed innate spindle motor....
Within microfluidic technologies, the centrifugal “Lab-on-a-Disc” (LoaD) platform offers great potential for use at PoC and in low-resource settings due to its robustness ability port miniaturize ‘wet bench’ laboratory protocols. We present combination of ‘event-triggered dissolvable film valves’ with a centrifugo-pneumatic siphon structure enable control timing, through changes disc spin-speed, release incubations eight samples/reagents/wash buffers. Based on these techniques, we integrated...
Cellular activation and inflammation leading to endothelial dysfunction is associated with cardiovascular disease (CVD). We investigated whether a single cell label-free multi parameter optical interrogation system can detect progenitor (EPC) in vitro ex vivo, respectively. Cultured human cells were exposed increasing concentrations of tumour necrosis factor alpha (TNF-α) or lipopolysaccharide (LPS) before was validated using fluorescence-activated sorting (FACS) analysis inflammatory marker...
Abstract Following the first deuterium–tritium experiment (DTE1) at UKAEA, modifications were investigated to Joint European Torus’ (JET) gas introduction (GI) systems increase feed capabilities of JET facility better support future campaigns. The GI comprise and distribution system in Active Gas Handling System (AGHS), neutral beam injection tritium modules on torus. During DTE1 about 100 g was supplied from AGHS users. second (DTE2) tritium–tritium (TT) just over 373 630 respectively users...
It is now widely recognised that the earliest changes occur on a cell when it stressed or becoming diseased are alterations in its surface glycosylation. Current state-of-the-art technologies glycoanalysis include mass spectrometry, protein microarray formats, techniques cytometry and more recently, glyco-quantitative polymerase chain reaction (Glyco-qPCR). Techniques for glycoprofiling of surfaces single cells either limited to analysis large populations unable handle multiple and/or...
Point-of-care (POC) testing attracts more and attention in the medical health sector because of their specific property to perform diagnostic close patient. The fast diagnosis right at hospital or doctor's office improves reaction time chances for a successful healing process. One this POC test systems is "Lab-on-a-Disc" (LoaD) which looks like compact disc crisscrossed with microfluidic tubes cavities. fluid be analysed placed LoaD an external device then rotates LoaD. cavities inside...
Abstract A hallmark of subclinical atherosclerosis is the accumulation vascular smooth muscle cell (SMC)-like cells leading to intimal thickening and lesion formation. While medial SMCs contribute lesions, involvement resident stem (vSCs) remains unclear. We evaluated single photonics as a discriminator phenotype in vitro before presence vSC within lesions was assessed ex vivo using supervised machine learning further validated lineage tracing analysis . Using novel lab-on-a-Disk(Load)...
ABSTRACT NK cell mimics are assemblies of a membrane and template that replicate biomimetic features physicochemical properties, respectively. For the reported design, we used from human (KHYG-1) line gelatin microspheres as template. The were reinforced via DMTMM cross-linking in water-in-oil emulsion to exhibit tunable Young’s modulus. These engineered found be non-toxic, non-inflammatory, capable evading macrophage detection when tested with differentiated THP-1 cells. In vitro studies...
Here we present integrated Lab-on-a-Disc (LoaD) cartridges which are applied to the purification of nucleic acid using silica bead based method. We utilize a novel combination `event-triggered' dissolvable film (DF) valving [1] and centrifugo-pneumatic siphon valve (CPSV) [2] permit timing sample incubations, washes DNA elution. two systems; one in with conventional `wash through' elution achieves yield 32.3% ± 5.4% (n = 4). The second system, uses CPSV enable extended wash steps, provides...
Increased demands for protein production efficiency by identification of optimal producer cells and more stringent FDA requirements regarding single cell clonality represent key unmet needs within the biopharma industry. A microfluidic analysis device is reported that identifies producing a population, subsequently isolating culturing them efficient line development. This lab on disc first centrifugally sediments cohort suspended CHO into an array 1,200 traps at single-occupancy distribution...
This protocol provides the required information to obtain a single cell analysis pro le for HUVEC which have been exposed differential levels of induced in ammation via lipopolysaccharide \(LPS) exposure.Reagents **Reagent preparation protocol** Differential has implemented by exposing cells various concentrations LPS during culture process \(below).LPS was obtained from Sigma Aldrich Ireland \(L2654-1MG) and diluted nal stock concentration 1,000 μg/mL.A further dilution diluent at ratio 1:5...
The LiPhos project (EU FP7 Grant Agreement No.: 317916, www.liphos.eu) aims to develop three different biophotonic diagnosis tools (BDTs), based on living photonics, namely: single layer photonics (SLLP), cell analysis (SCA); and multi-layer (MLLP). By Measuring of what we term the Photonic Fingerprint (or PIN), cells in such BDTs, should make it is possible differentiate between healthy non-healthy or tissue states. Moreover, effect specific drugs pro-inflammatory agents could be assessed....