A5048043076- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Circular RNAs in diseases
- Copper Interconnects and Reliability
- Gene Regulatory Network Analysis
- Protein purification and stability
- Electrochemical Analysis and Applications
- Extraction and Separation Processes
- Trace Elements in Health
- MicroRNA in disease regulation
- Genomics and Phylogenetic Studies
- Nanowire Synthesis and Applications
- Molecular Biology Techniques and Applications
- Virus-based gene therapy research
- Advanced Proteomics Techniques and Applications
Dublin City University
2017-2024
La Jolla Alcohol Research
2024
Scripps Research Institute
2024
Broad Institute
2020-2021
Harvard University
2021
Science Foundation Ireland
2019
Abstract Supplementing translation with non-canonical amino acids (ncAAs) can yield protein sequences new-to-nature functions, but existing ncAA incorporation strategies suffer from low efficiency and context dependence. We uncover codon usage as a previously unrecognized contributor to efficient genetic code expansion using non-native codons. Relying only on conventional E. coli strains native ribosomes, we develop novel plasmid-based compression strategy that minimizes dependence improves...
With the ability to affect multiple genes and fundamental pathways simultaneously, miRNA engineering of Chinese Hamster Ovary (CHO) cells has significant advantages over single gene expression or repression. Tight control these molecular triggers is desirable as it could in theory allow on/off even tunable regulation cellular phenotypes. The present study investigated potential employing a tetracycline inducible (TET-On) system for conditional knockdown specific miRNAs but encountered...
In bacteria, ribosome kinetics are considered rate-limiting for protein synthesis and cell growth. Enhanced may augment bacterial growth biomanufacturing through improvements to overall yield, but whether this can be achieved by ribosome-specific modifications remains unknown. Here, we evolve 16S ribosomal RNAs (rRNAs) from Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae towards enhanced rates. We find that rRNA sequence origin significantly impacted evolutionary trajectory...
Abstract RNA sequencing (RNASeq) has been widely used to associate alterations in Chinese hamster ovary (CHO) cell gene expression with bioprocess phenotypes; however, alternative messenger (mRNA) splicing, thus far, received little attention. In this study, we utilized RNASeq for transcriptomic analysis of a monoclonal antibody (mAb) producing CHO K1 line subjected temperature shift. More than 2,465 instances differential splicing were observed 24 hr after the reduction culture temperature....
The proteomic data presented in this article provide supporting information to the related research "Depletion of endogenous miRNA-378-3p increases peak cell density CHO DP12 cells and is correlated with elevated levels Ubiquitin Carboxyl-Terminal Hydrolase 14" (Costello et al., press) [1]. Control microRNA-378 depleted were profiled using label-free quantitative profiling. collected on day 4 8 batch culture, subcellular enrichment was performed, subsequent fractions analyzed by liquid...
MicroRNAs (miRNAs) regulate approximately one-third of all human genes. The dysregulation miRNAs has been implicated in the development numerous diseases, including cancers. In our investigation focusing on altering specific miRNA expression pancreatic cancer cells, we encountered an interesting finding. While two vector designs effectively enhanced miR-708 levels, they were unable to elevate mature forms miR-29b, -1290, -2467, and -6831 cell lines. This finding was also observed a panel...
The modern world has seen exposure of bacterial communities to toxic metals at selective levels. This manifests itself both intentionally, through medicines and un-intentionally waste streams. There is growing concern that may be linked microbial resistance antibiotics. For a microbe become resistant specific metal it must first come in contact with it. transition copper the ability enter cells without need for uptake mechanism. Copper commonly used as an antimicrobial healthcare industry,...
Abstract RNA sequencing (RNASeq) has been widely used to associate alterations in Chinese hamster ovary (CHO) cell gene expression with bioprocess phenotypes, however alternative mRNA splicing, thus far, received little attention. In this study, we utilised RNASeq for transcriptomic analysis of a monoclonal antibody producing CHOK1 line subjected temperature shift. More than 2,365 instances differential splicing were observed 24hrs after the reduction culture temperature. 1,163 these events...