A5088540624- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Analytical Chemistry and Chromatography
- Virus-based gene therapy research
- Mass Spectrometry Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Carbohydrate Chemistry and Synthesis
- Analytical chemistry methods development
- Advanced Proteomics Techniques and Applications
- RNA Interference and Gene Delivery
- Pharmaceutical and Antibiotic Environmental Impacts
- Biosimilars and Bioanalytical Methods
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Multiple Myeloma Research and Treatments
- Proteoglycans and glycosaminoglycans research
- CAR-T cell therapy research
- Galectins and Cancer Biology
- RNA and protein synthesis mechanisms
- Single-cell and spatial transcriptomics
- Machine Learning in Bioinformatics
- CRISPR and Genetic Engineering
- Erythrocyte Function and Pathophysiology
National Institute for Bioprocessing Research and Training
2016-2025
University College Dublin
2012-2025
University of Limerick
2016-2019
22q11 Ireland
2019
Northeastern University
2011-2015
Character
2015
BlackRock (United States)
2013
Rutherford Appleton Laboratory
2011
University of Debrecen
2010
Lonza (United Kingdom)
2010
Plasma glycans were analyzed in 1008 individuals to evaluate variability and heritability, as well the main environmental determinants that affect glycan structures. By combining HPLC analysis of fluorescently labeled with sialidase digestion, separated into 33 chromatographic peaks quantified. A high level was observed median ratio minimal maximal values 6.17 significant age- gender-specific differences. Heritability estimates for individual varied widely, ranging from very low high....
A solid phase extraction (SPE) method has been developed and applied in conjunction with a previously reported liquid chromatography tandem mass spectrometry (LC-MS-MS) procedure for the determination of illicit drugs abused pharmaceuticals treated wastewater surface water samples at ng L(-1) level. full validation was also performed determined levels analytical sensitivity were found to lie 1-10 range using river as test sample matrix size 500 mL. The successfully chosen analytes treatment...
Glycosylation is a diverse but critically important post-translational modification that modulates the physical, chemical and biological properties of proteins. Alterations in glycosylation have been noted number diseases including cancer. The discovery alterations serum glycoproteins which may offer potential as biomarkers attracting considerable research interest. In current study, significant improvements efficiency, selectivity, analysis speed offered by ultra performance liquid...
Despite the reduced incidence of gastric cancer in developed world, a diagnosis stomach carcinoma still carries poor prognosis due to asymptomatic nature disease early stages, subsequent advanced stage diagnosis, and low 5 year survival rate. Endoscopy remains primary standard for current marker, carbohydrate antigen 19-9 (CA19-9) lacks levels sensitivity specificity required order make it clinically useful diagnostic monitoring. Therefore, there is need additional markers improve accuracy...
Charge variant analysis (CVA) of monoclonal antibodies (mAbs) using cation exchange chromatography is routinely used as a fingerprint the distribution posttranslational modifications present on molecule. Traditional salt or pH based eluents are not suited for direct coupling to mass spectrometry due nonvolatility high ionic strength. This makes further complicated when an alteration in charge profile emergence additional peak encountered. Here, use gradient elution volatile, low strength...
Charge variant analysis is a widely used tool to monitor changes in product quality during the manufacturing process of monoclonal antibodies (mAbs). Although it powerful technique for revealing mAb heterogeneity, an unexpected outcome, example appearance previously undetected isoforms, requires further, time-consuming analysis. The identifying these unknowns can also result unwanted molecule that are not attributable process. To overcome this, we recently reported method combining highly...
Charge sensitive separation methods such as ion exchange chromatography (CEX) and capillary electrophoresis (CE) have recently been coupled to mass spectrometry facilitate high resolution profiling of proteoforms present within the charge variant profile complex biopharmaceuticals. Here we apply pH gradient cation microfluidic using ZipChip platform for comparative characterization monoclonal antibody Cetuximab. Cetuximab harbors four glycans per molecule, two on each heavy chain, which Fab...
Host cell proteins (HCPs) are bioprocess-related impurities arising from cell-death or secretion nonhuman cells used for recombinant protein production. Clearance of HCPs through downstream purification (DSP) is required to produce safe and efficacious therapeutic proteins. While traditionally measured using anti-HCP ELISA, more in-depth approaches HCP characterization may ensure that risks patients adequately assessed. Mass spectrometry methods provide rationale targeted removal strategies...
Abstract Peptide mapping analysis is a regulatory expectation to verify the primary structure of recombinant product sequence and monitor post-translational modifications (PTMs). Although proteolytic digestion has been used for decades, it remains labour-intensive procedure that can be challenging accurately reproduce. Here, we describe fast reproducible protocol protease automated using immobilised trypsin on magnetic beads, which incorporated into an optimised peptide workflow show method...
The multi-attribute method (MAM) is a liquid chromatography-mass spectrometry based that used to directly characterize and monitor many product quality attributes impurities on biotherapeutics, most commonly at the peptide level. It utilizes high-resolution accurate mass spectral data which are analyzed in an automated fashion. MAM promising approach intended replace or supplement several conventional assays with single LC-MS analysis can be implemented Current Good Manufacturing Practice...
N-glycans attached to the C(H)2 domains of Fc or antigen binding regions IgG play an important role in stabilizing and modulating antibody activity. Exhaustive elucidation 32 using a combination weak anion exchange enrichment exoglycosidase array digestion with subsequent profiling exceeded 48 h. Pursuing increased throughput associated structural annotation confidence, we compared 1.7 μm hydrophilic interaction phase for UPLC CE-LIF rapid comprehensive characterization released from healthy...
Recombinant human erythropoietin has been used to treat anemia associated with chronic renal disease. This paper provides a comprehensive comparative analysis of Dynepo and three other commercial erythropoiesis stimulating agents, Eprex, NeoRecormon Aranesp. We found significant differences in the type, levels amount O-acetylation sialic acids. Sialic acids present provide protection from clearance circulation. Aranesp had up six O-acetyl groups attached Eprex only minor amounts while none....
State-of-the-art high-resolution separation techniques play an important role in the full structural elucidation of glycans. Capillary electrophoresis (CE) offers a rapid yet simple method for exhaustive carbohydrate profiling. CE is versatile analytical platform, which can be operated several modes, simply by altering conditions during operation. For in-depth glycan analysis, has also gained significantly from additional resolution introduced complementary and orthogonal such as ion...
The repertoire of complex proteins produced by the host cell during monoclonal antibody (mAb) production has generated a bottleneck in downstream bioprocessing. Low ppm levels (HCPs) must be achieved at purification process stage to generate an end product suitable for use humans. increased demand mAb drug products globally driven research focus on affordability platforms. This fuelled advancements manufacturing R&D deliver higher titres with better economics without sacrificing quality....