A5039536102- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Cellular transport and secretion
- Viral Infectious Diseases and Gene Expression in Insects
- Lysosomal Storage Disorders Research
- Nanoparticles: synthesis and applications
- Advanced Nanomaterials in Catalysis
- Carbohydrate Chemistry and Synthesis
- Advanced biosensing and bioanalysis techniques
- Bacterial Genetics and Biotechnology
- Virus-based gene therapy research
- Air Quality and Health Impacts
- Calcium signaling and nucleotide metabolism
- Protein purification and stability
Universitat Autònoma de Barcelona
2019-2024
Instituto de Salud Carlos III
2024
Institut de Ciència de Materials de Barcelona
2022-2024
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2022-2024
Abstract Transient gene expression (TGE) in mammalian cells is a well-known approach to the fast of recombinant proteins. The human cell line HEK (human embryonic kidney) 293F widely used this field, due its adaptability grow suspension high densities serum-free media, amenability transfection, and production proteins satisfactory quantities for functional structural analysis. Amounts plasmid DNA (pDNA) required transfections TGE remain (usually 1 µg pDNA/mL, or even higher), representing...
Abstract One of the most promising approaches in drug delivery field is use naturally occurring self-assembling protein nanoparticles, such as virus-like particles, bacterial microcompartments or vault ribonucleoprotein particles systems (DDSs). Among them, eukaryotic vaults show a future due to their structural features, vitro stability and non-immunogenicity. Recombinant are routinely produced insect cells purified through several ultracentrifugations, both tedious time-consuming...
Endotoxin contamination is a significant hurdle to the translation of nanomaterials for biomedical applications.
The central nervous system represents a major target tissue for therapeutic approach of numerous lysosomal storage disorders. Fabry disease arises from the lack or dysfunction alpha-galactosidase A (GLA) enzyme, resulting in substrate accumulation and multisystemic clinical manifestations. Current enzyme replacement therapies (ERTs) face limited effectiveness due to poor biodistribution tissues inability reach brain. We present an innovative drug delivery strategy centered on...
Vaults are protein nanoparticles that found in almost all eukaryotic cells but absent prokaryotic ones. Due to their properties (nanometric size, biodegradability, biocompatibility, and lack of immunogenicity), vaults show enormous potential as a bio-inspired, self-assembled drug-delivery system (DDS). Vault architecture is directed by self-assembly the "major vault protein" (MVP), main component this nanoparticle. Recombinant expression (in different systems) MVP resulted formation were...