A5081499334- Hearing, Cochlea, Tinnitus, Genetics
- Effects and risks of endocrine disrupting chemicals
- Adipose Tissue and Metabolism
- Cancer-related molecular mechanisms research
- Marine animal studies overview
- Developmental Biology and Gene Regulation
- Birth, Development, and Health
- Peroxisome Proliferator-Activated Receptors
- Retinoids in leukemia and cellular processes
- Adipokines, Inflammation, and Metabolic Diseases
- Animal Vocal Communication and Behavior
- Single-cell and spatial transcriptomics
- Toxic Organic Pollutants Impact
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Per- and polyfluoroalkyl substances research
- Reproductive System and Pregnancy
- Calcium signaling and nucleotide metabolism
- Bat Biology and Ecology Studies
- Immune Cell Function and Interaction
- Antimicrobial Peptides and Activities
- IL-33, ST2, and ILC Pathways
- Cancer Cells and Metastasis
- Neurogenesis and neuroplasticity mechanisms
- Congenital heart defects research
10X Genomics (United States)
2022-2024
Stanford University
2017-2023
Institute for Stem Cell Biology and Regenerative Medicine
2021
University of California, Irvine
2011-2020
Single-cell and spatial technologies that profile gene expression across a whole tissue are revolutionizing the resolution of molecular states in clinical samples. Current commercially available provide transcriptome single-cell, spatial, or targeted situ analysis. Here, we combine these to explore heterogeneity large, FFPE human breast cancer sections. This integrative approach allowed us differences exist between distinct tumor regions identify biomarkers involved progression towards...
Abstract Single cell and spatial technologies that profile gene expression across a whole tissue are revolutionizing the resolution of molecular states in clinical samples. Commercially available methods characterize either single or currently limited by low sample throughput and/or plexy, lack on-instrument analysis, destruction histological features epitopes during workflow. Here, we analyzed large, serial formalin-fixed, paraffin-embedded (FFPE) human breast cancer sections using novel...
Background: Bisphenol A (BPA) and bisphenol diglycidyl ether (BADGE), used in manufacturing coatings resins, leach from packaging materials into food. Numerous studies suggested that BPA BADGE may have adverse effects on human health, including the possibility exposure to such chemicals can be superimposed traditional risk factors initiate or exacerbate development of obesity. is a suspected obesogen, whereas BADGE, described as peroxisome proliferator–activated receptor gamma (PPARγ)...
Recent studies suggest that exposure to endocrine-disrupting compounds (EDCs) may play a role in the development of obesity. EDCs such as flame retardant 2,2',4,4'-tetrabrominated diphenyl ether (BDE-47) have been shown enhance adipocyte differentiation murine 3T3-L1 model. The mechanisms by which direct preadipocytes form adipocytes are poorly understood. Here, we examined transcriptional and epigenetic underlying induction vitro BDE-47. Quantitative high content microscopy revealed...
Background:In ToxCast™ Phase I, the U.S. EPA commissioned screening of 320 pesticides, herbicides, fungicides, and other chemicals in a series high-throughput assays. The agency also developed toxicological prioritization tool, ToxPi, to facilitate using assays predict biological function.Objectives:We asked whether top-scoring PPARγ activators identified I were genuine inducers adipogenesis. Next, we that should adipogenesis, an adipogenesis how well ToxPi predicted adipogenic...
Triflumizole (TFZ) is an imidazole fungicide used on many food and ornamental crops. TFZ not thought to be particularly toxic or carcinogenic, but little known about its effect development. identified as a peroxisome proliferator activated receptor gamma (PPARγ) activator in ToxCast. Because PPARγ master regulator of adipogenesis, we hypothesized that would activate PPARγ, thereby inducing adipogenesis weight gain vivo.We sought test the ability promote vitro transient transfection 3T3-L1...
Background: The organotin dibutyltin (DBT) is used in the manufacture of polyvinyl chloride (PVC) plastics, construction materials, and medical devices. Previous animal studies showed detrimental effects DBT during utero development at relatively high doses, but little was known about exposure environmentally relevant doses on endpoints such as obesity metabolic disease. Objectives: We tested potential obesogenic using vitro vivo models. Methods. evaluated nuclear receptor activation...
In contrast to mammals, birds recover naturally from acquired hearing loss, which makes them an ideal model for inner ear regeneration research. Here, we present a validated single-cell RNA sequencing resource of the avian cochlea. We describe specific markers three distinct types sensory hair cells, including previously unknown subgroup, call superior tall cells. identify supporting cells associated with represent facultative stem ear. Likewise, that are located below short cochlear further...
ABSTRACT The avian hearing organ is the basilar papilla that, in sharp contrast to mammalian cochlea, can regenerate sensory hair cells and thereby recover from deafness within weeks. mechanisms that trigger, sustain terminate regenerative response vivo are largely unknown. Here, we profile changes gene expression chicken after aminoglycoside antibiotic-induced cell loss using RNA-sequencing. We identified of a group immune-related genes confirmed with single-cell RNA-sequencing these occur...
Retinoic acid signaling is a major component of the neural posteriorizing process in vertebrate development. Here, we identify new role for retinoic receptor (RAR) anterior embryo, where RAR regulates Fgf8 expression and formation pre-placodal ectoderm (PPE). RARα2 induces key genes establishes posterolateral borders PPE. upregulates two important genes, Tbx1 Ripply3, during early PPE In absence RIPPLY3, TBX1 required hence, formation. presence acts as transcriptional repressor, functions to...
// Jun Wang 1,2 , Weina Bi 1 Wei Zhao Merina Varghese Rick J. Koch 3 Ruth H. Walker Roshantha A. Chandraratna 4 Martin E. Sanders Amanda Janesick 5 Bruce Blumberg Libby Ward Lap Ho and Giulio M. Pasinetti Department of Neurology, Icahn School Medicine at Mount Sinai, New York, NY, USA 2 Geriatric Research, Education Clinical Center, Bronx, James Peters Veterans Affairs Medical Io Therapeutics Inc., Santa Ana, CA, Departments Developmental Cell Biology, Pharmaceutical Sciences, University...
Tetrabromobisphenol A (TBBPA) is the most common flame retardant used in electrical housings, circuit boards, and automobiles. High-throughput screening binding assays have identified TBBPA as an agonist for human peroxisome proliferator-activated receptor gamma (PPARγ), master regulator of adipogenesis. has been suggested to be obesogen based on vitro cellular zebrafish data. We hypothesized that exposing preadipocytes could influence adipogenesis via genes other than those PPARγ pathway...
Sensory hair cells are prone to apoptosis caused by various drugs including aminoglycoside antibiotics. In mammals, this vulnerability results in permanent hearing loss because lost not regenerated. Conversely, regenerate birds, making the avian inner ear an exquisite model for studying ototoxicity and regeneration. Here, we use single-cell RNA sequencing trajectory analysis on control dying after treatment. Interestingly, two major subtypes of cochlear cells, tall short respond differently....
Retinoic acid receptor gamma 2 (RARγ2) is the major RAR isoform expressed throughout caudal axial progenitor domain in vertebrates. During a microarray screen to identify targets, we identified subset of genes that pattern structures or promote elongation and are upregulated by increased RAR-mediated repression. Previous studies have suggested present domain, but quiescent until its activation late stage embryos terminates elongation. By contrast, show here RARγ2 engaged all stages...
Lysosomes contribute to cellular homeostasis via processes including macromolecule degradation, nutrient sensing, and autophagy. Defective proteins related lysosomal catabolism are known cause a range of storage diseases; however, it is unclear whether mutations in involved homeostatic sensing mechanisms syndromic sensory disease. Here, we show that SLC7A14, transporter protein mediating uptake cationic amino acids, evolutionarily conserved vertebrate mechanosensory hair cells highly...
The avian utricle, a vestibular organ of the inner ear, displays turnover sensory hair cells throughout life. This is in sharp contrast to mammalian which shows limited regenerative capacity. Here, we use single-cell RNA sequencing identify distinct marker genes for different cell subtypes chicken validated situ. We provide markers spatially supporting populations and two transitional dedifferentiating developing cells. Trajectory reconstruction resulted an inventory gene expression dynamics...