A5030810079- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Poxvirus research and outbreaks
- Malaria Research and Control
- Adipose Tissue and Metabolism
- Cytomegalovirus and herpesvirus research
- PI3K/AKT/mTOR signaling in cancer
- Toxin Mechanisms and Immunotoxins
- Parasitic Infections and Diagnostics
- Effects and risks of endocrine disrupting chemicals
- Genomics and Chromatin Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Toxic Organic Pollutants Impact
- Digital Imaging for Blood Diseases
- Sustainable Supply Chain Management
- Sepsis Diagnosis and Treatment
- Streptococcal Infections and Treatments
- Urban Heat Island Mitigation
- Mosquito-borne diseases and control
- Municipal Solid Waste Management
- Coccidia and coccidiosis research
- Ubiquitin and proteasome pathways
- Neonatal and Maternal Infections
- Per- and polyfluoroalkyl substances research
- Cancer, Hypoxia, and Metabolism
University of British Columbia
2013
University of California, Irvine
2010
Harvard University
1993-2006
Brigham Young University
1991-1993
Massachusetts Institute of Technology
1993
The environmental obesogen hypothesis proposes that pre- and postnatal exposure to chemicals contributes adipogenesis the development of obesity. Tributyltin (TBT) is an agonist both retinoid X receptor (RXR) peroxisome proliferator-activated gamma (PPARgamma). Activation these receptors can elevate adipose mass in adult mice exposed chemical utero. Here we show TBT sensitizes human mouse multipotent stromal stem cells derived from white tissue [adipose-derived (ADSCs)] undergo adipogenesis....
High-mobility-group 1 protein (HMG1) is an abundant eukaryotic DNA-binding protein, the cellular role of which remains ill-defined. To test ability HMG1 itself to mediate curvature in double-stranded DNA, we examined its effect on phage T4 DNA ligase-dependent cyclization short fragments. caused circle formation for fragments > or = 87 bp. Fragments 123, 100, 92, and bp did not cyclize absence but formed covalently closed circular monomers efficiently presence HMG1, indicating that...
Background: Bisphenol A (BPA) and bisphenol diglycidyl ether (BADGE), used in manufacturing coatings resins, leach from packaging materials into food. Numerous studies suggested that BPA BADGE may have adverse effects on human health, including the possibility exposure to such chemicals can be superimposed traditional risk factors initiate or exacerbate development of obesity. is a suspected obesogen, whereas BADGE, described as peroxisome proliferator–activated receptor gamma (PPARγ)...
The herpes simplex virus UL42 gene encodes a multifunctional polypeptide (UL42) that is essential for DNA replication. To further understand the relationship between structure of and role it plays during replication, we analyzed an extensive set mutant proteins ability to perform three major biochemical functions ascribed protein:binding DNA, stably associating with polymerase (Pol), acting increase length chains synthesized by Pol. Selected mutants were also assayed their complement...
Herpes simplex virus DNA polymerase consists of a catalytic subunit, Pol, and processivity UL42, that, unlike other established factors, binds directly. We used gel retardation filter-binding assays to investigate how UL42 affects the polymerase-DNA interaction. The Pol/UL42 heterodimer bound more tightly in primer-template configuration than single-stranded (ssDNA), while Pol alone ssDNA configuration. affinity for was reduced severalfold relative that increased approximately 15-fold Pol....
ABSTRACT Herpes simplex virus DNA polymerase consists of a catalytic subunit, Pol, and processivity UL42, that, unlike other established factors, binds directly. We used gel retardation filter-binding assays to investigate how UL42 affects the polymerase-DNA interaction. The Pol/UL42 heterodimer bound more tightly in primer-template configuration than single-stranded (ssDNA), while Pol alone ssDNA configuration. affinity for was reduced severalfold relative that increased ∼15-fold Pol....
The herpes simplex virus DNA polymerase is a heterodimer consisting of catalytic subunit and the protein UL42, which functions as processivity factor. It has been hypothesized that UL42 tethers to template by virtue binding activity (J. Gottlieb, A. I. Marcy, D. M. Coen, Challberg, J. Virol. 64:5976-5987, 1990). Relevant this hypothesis, we identified two linker insertion mutants were unable bind double-stranded-DNA-cellulose column but retained their ability subunit. These severely impaired...
ABSTRACT The catalytic subunit, Pol, of herpes simplex virus DNA polymerase interacts via its extreme C terminus with the processivity UL42. This interaction is critical for viral replication and thus a potential target antiviral drug action. To investigate Pol-binding region on UL42, we engineered UL42 mutations but also used random peptide display to identify artificial ligands Pol terminus. latter approach selected homology residues 171 176 Substitution glutamine alanine greatly impaired...
The environmental obesogen hypothesis proposes that pre- and postnatal exposure to chemicals contributes adipogenesis the development of obesity. Tributyltin (TBT) is an agonist both retinoid X receptor (RXR) peroxisome proliferator-activated (PPAR). Activation these receptors can elevate adipose mass in adult mice exposed chemical utero. Here we show TBT sensitizes human mouse multipotent stromal stem cells derived from white tissue [adipose-derived (ADSCs)] undergo adipogenesis. In vitro...
Detection of herpes simplex virus in clinical specimens by the suspension-infection technique was compared with detection conventional cell culture. The sensitivity and specificity those culture were 95.9 to 98.2% 97.5 100%, respectively, depending on line used tests. mean time diagnosis 1 day, 4.8 days Comparable low-level positive observed methods. In a setting, isolation rates obtained indistinguishable. These results indicate that method can be for yield rapid diagnostic without...
During September-October 2021, group B Streptococcus bloodstream infections surged among patients hospitalized in Hong Kong. Of 95 cases, 57 were caused by the hypervirulent strain sequence type 283, which at time was also found freshwater fish and wet market environments thus poses a transmission threat.