A5019889467- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Plant responses to water stress
- Plant Parasitism and Resistance
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Turfgrass Adaptation and Management
- Autophagy in Disease and Therapy
- Legume Nitrogen Fixing Symbiosis
- Plant tissue culture and regeneration
- GABA and Rice Research
- Plant Gene Expression Analysis
- Plant and Biological Electrophysiology Studies
- Lipid metabolism and biosynthesis
- Studies on Chitinases and Chitosanases
- Enzyme-mediated dye degradation
- Aluminum toxicity and tolerance in plants and animals
- Ion Channels and Receptors
- Plant Pathogenic Bacteria Studies
- Calcium signaling and nucleotide metabolism
- Ion channel regulation and function
- Vibrio bacteria research studies
- Plant pathogens and resistance mechanisms
Suwa Red Cross Hospital
2019-2025
Tokyo University of Science
2013-2023
Tokyo University of Technology
2013-2018
Faculty of Media
2016
Institute of Agrobiological Sciences
2010
Japan Science and Technology Agency
2005
The University of Tokyo
2002
Nippon Suisan Kaisha (Japan)
1996
In flowering plants, the tapetum, innermost layer of anther, provides both nutrient and lipid components to developing microspores, pollen grains, coat. Though programmed cell death tapetum is one most critical sensitive steps for fertility affected by various environmental stresses, its regulatory mechanisms remain mostly unknown. Here we show that autophagy required metabolic regulation supply in anthers autophagic degradation within cells essential postmeiotic anther development rice....
Much of the nitrogen in leaves is distributed to chloroplasts, mainly photosynthetic proteins. During leaf senescence, chloroplastic proteins, including Rubisco, are rapidly degraded, and released remobilized reused newly developing tissues. Autophagy facilitates degradation intracellular components for nutrient recycling all eukaryotes, recent studies have revealed critical roles autophagy Rubisco remobilization into seeds Arabidopsis (Arabidopsis thaliana). Here, we examined function...
Abstract Background Mechanosensing and its downstream responses are speculated to involve sensory complexes containing Ca 2+ -permeable mechanosensitive channels. On recognizing osmotic signals, plant cells initiate activation of a widespread signal transduction network that induces second messengers triggers inducible defense responses. Characteristic early signaling events include influx, protein phosphorylation generation reactive oxygen species (ROS). Pharmacological analyses show influx...
Autophagy is an intracellular process leading to vacuolar or lysosomal degradation of cytoplasmic components in eukaryotes. Establishment proper methods monitor autophagy was a key step uncovering its role organisms, such as yeast (Saccharomyces cerevisiae), mammals, and Arabidopsis (Arabidopsis thaliana), which chloroplastic proteins were found be recycled by autophagy. Chloroplast recycling has been predicted function nutrient remobilization for growing organs grain filling cereal crops....
Summary Elicitor‐triggered transient membrane potential changes and Ca 2+ influx through the plasma are thought to be important during defense signaling in plants. However, molecular bases for its regulation remain largely unknown. Here we tested effects of overexpression as well retrotransposon ( Tos17 )‐insertional mutagenesis rice two‐pore channel 1 (OsTPC1), a putative voltage‐gated ‐permeable channel, on proteinaceous fungal elicitor‐induced responses cells. The overexpressor showed...
Abstract Although cytosolic free Ca2+ mobilization induced by microbe/pathogen-associated molecular patterns is postulated to play a pivotal role in innate immunity plants, the links between and downstream defense responses still remain largely unknown. Calcineurin B-like proteins (CBLs) act as sensors activate specific protein kinases, CBL-interacting kinases (CIPKs). We here identified two CIPKs, OsCIPK14 OsCIPK15, rapidly microbe-associated patterns, including chitooligosaccharides...
The turf grass Sporobolus virginicus is halophyte and has high salinity tolerance. To investigate the molecular basis of its remarkable tolerance, we performed Illumina high-throughput RNA sequencing on roots shoots a S. genotype under normal saline conditions. 130 million short reads were assembled into 444,242 unigenes. A comparative analysis transcriptome with rice Arabidopsis revealed six grass-specific unigenes encoding transcription factors. Interestingly, all them showed root specific...
Autophagy has recently been shown to be required for postmeiotic anther development including dehiscence, programmed cell death-mediated degradation of the tapetum and pollen maturation in rice. Several phytohormones are known play essential roles during male reproductive maturation. However, relationship between phytohormone metabolism autophagy plant is unknown. We here comprehensively analyzed effect disruption on contents rice anthers at flowering stage, found that endogenous levels...
Abstract Autophagy plays crucial roles in the recycling of metabolites, and is involved many developmental processes. Rice mutants defective autophagy are male sterile due to immature pollens, indicating its critical role pollen development. However, physiological during seed maturation had remained unknown. We here found that seeds rice autophagy-deficient mutant Osatg7-1 , produces at a very low frequency paddy fields, smaller show chalky appearance lower starch content endosperm mature...
Cytosolic free Ca2+ serves as an important second messenger participating in signal transduction of various environmental stresses. However, molecular bases for the plasma membrane influx and its regulation remain largely unknown. We here identified a gene (OsTPC1) encoding putative voltage-gated channel from rice, ubiquitously expressed mature leaves, shoots roots well cultured cells. OsTPC1 rescued uptake activity growth rate yeast mutant cch1. To elucidate physiological roles, we...
Cytosolic free Ca2+ mobilization induced by microbe/pathogen-associated molecular patterns (MAMPs/PAMPs) play key roles in plant innate immunity. However, components involved signaling pathways still remain to be identified and possible involvement of the CBL (calcineurin B-like proteins)-CIPK (CBL-interacting protein kinases) system biotic defense has yet clarified. Recently we two CIPKs, OsCIPK14 OsCIPK15, which are rapidly MAMPs, various MAMP-induced immune responses including...
Understanding the mechanisms used by halophytic members of Poaceae to cope with salt stress will contribute knowledge necessary genetically engineer salt-tolerant crops. In this study, we identified a genotype Sporobolus virginicus, turf grass collected in Japan, and investigated its growth rate, ion concentration secretion, proline comparison reported properties genotypes from USA, South Africa Egypt. Surprisingly, Japanese showed salinity tolerance up 1.5 M NaCl, 3-fold higher than...
Summary The slow vacuolar ( SV ) channel has been characterized in different dicots by patch‐clamp recordings. This represents the major cation conductance of largest organelle most plant cells. Studies with tpc1‐2 mutant model dicot Arabidopsis thaliana identified as product TPC 1 gene. By contrast, research on rice and wheat suggested that monocot gene encodes a plasma membrane calcium‐permeable channel. To explore site action grass channels, we expressed Os from Oryza sativa) Ta Triticum...
Pharmacological evidence suggests that anion channel-mediated plasma membrane effluxes are crucial in early defense signaling to induce immune responses and hypersensitive cell death plants. However, their molecular bases regulation remain largely unknown. We overexpressed Arabidopsis SLAC1, an S-type channel involved stomatal closure, cultured tobacco BY-2 cells analyzed the effect on cryptogein-induced including fluxes of Cl− other ions, production reactive oxygen species (ROS), gene...
Autophagy has been shown to play essential roles in the growth, development and survival of eukaryotic cells. However, simple methods for quantification visualization autophagic flux remain be developed living plant Here, we analyzed transgenic tobacco BY-2 cell lines expressing fluorescence-tagged NtATG8a as a marker autophagosome formation. Under sucrose-starved conditions, number punctate signals YFP-NtATG8a increased, fluorescence intensity cytoplasm nucleoplasm decreased. Conversely,...
Two-pore channels (TPCs) are cation with a voltage-sensor domain conserved in plants and animals. Rice OsTPC1 is predominantly localized to the plasma membrane (PM), assumed play an important role as Ca2+-permeable channel regulation of cytosolic Ca2+ rise innate immune responses including hypersensitive cell death phytoalexin biosynthesis cultured rice cells triggered by fungal elicitor, xylanase from Trichoderma viride. In contrast, Arabidopsis AtTPC1 vacuolar (VM). To gain further...