A5051917348- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Photosynthetic Processes and Mechanisms
- Autophagy in Disease and Therapy
- Plant responses to water stress
- GaN-based semiconductor devices and materials
- Crystallography and molecular interactions
- Electrical Contact Performance and Analysis
- Semiconductor materials and devices
- Diamond and Carbon-based Materials Research
- Plant Stress Responses and Tolerance
- Adhesion, Friction, and Surface Interactions
- Advancements in Semiconductor Devices and Circuit Design
- Plant Gene Expression Analysis
- Radio Frequency Integrated Circuit Design
- High-pressure geophysics and materials
- Plant Molecular Biology Research
- Semiconductor Quantum Structures and Devices
- Mitochondrial Function and Pathology
- Silicon Carbide Semiconductor Technologies
- Mechanical stress and fatigue analysis
- Advancements in PLL and VCO Technologies
- Integrated Circuits and Semiconductor Failure Analysis
- Microwave Engineering and Waveguides
- Force Microscopy Techniques and Applications
Tohoku University
2014-2025
Keio University
2023-2024
National Institutes of Natural Sciences
2023
RIKEN Center for Sustainable Resource Science
2023
Hokkaido University
2023
Juntendo University
2023
National Institute for Physiological Sciences
2023
The Graduate University for Advanced Studies, SOKENDAI
2023
Cornell University
2006-2021
Panasonic (Japan)
2000-2019
Abstract Chloroplasts contain approximately 80% of total leaf nitrogen and represent a major source recycled during senescence. While bulk degradation the cytosol organelles in plants is mediated by autophagy, its role chloroplast catabolism largely unknown. We investigated effects autophagy disruption on number size chloroplasts When leaves were individually darkened, senescence was promoted similarly both wild-type Arabidopsis (Arabidopsis thaliana) an autophagy-defective mutant,...
Abstract During senescence and at times of stress, plants can mobilize needed nitrogen from chloroplasts in leaves to other organs. Much the total leaf is allocated most abundant plant protein, Rubisco. While bulk degradation cytosol organelles occurs by autophagy, role autophagy chloroplast proteins still unclear. We have visualized fate Rubisco, stroma-targeted green fluorescent protein (GFP) DsRed, GFP-labeled Rubisco order investigate involvement mobilization stromal vacuole. Using...
Turnover of dysfunctional organelles is vital to maintain homeostasis in eukaryotic cells. As photosynthetic organelles, plant chloroplasts can suffer sunlight-induced damage. However, the process for turnover entire damaged remains unclear. Here, we demonstrate that autophagy responsible elimination sunlight-damaged, collapsed Arabidopsis thaliana We found vacuolar transport chloroplasts, termed chlorophagy, was induced by UV-B damage chloroplast apparatus. This did not occur...
In flowering plants, the tapetum, innermost layer of anther, provides both nutrient and lipid components to developing microspores, pollen grains, coat. Though programmed cell death tapetum is one most critical sensitive steps for fertility affected by various environmental stresses, its regulatory mechanisms remain mostly unknown. Here we show that autophagy required metabolic regulation supply in anthers autophagic degradation within cells essential postmeiotic anther development rice....
Raman spectra of diamondlike amorphous carbon (a-C) films prepared under atmosphere with various hydrogen gas content have been measured as a function excitation wavelength. The spectral profiles vary wavelength depending on electronic absorption associated π-π* transitions. Dependence is interpreted in terms resonant scattering from aromatic rings sizes rather than polyene chains. relative intensity 1400 cm−1 band against 1530 found to decrease an increase sp3 a-C films. It shown that the...
We have measured Raman spectra of diamond with nanometer size, called cluster diamond. The bands assigned to sp2 and sp3 clusters been observed at around 1600 1322 cm−1, respectively. This result suggests that the slightly contains cluster. band is found shift by −10 compared bulk crystal be asymmetric some tailing toward lower frequency. spectrum agrees well calculated a phonon confinement model. crystallite size estimated from model approximately x-ray measurement. spectroscopy gives...
To realize an ultra wideband (UWB) bandpass filter (BPF) in wireless communications, a device is developed using new ring filter. The compact, with low insertion loss, sharp rejection and constant group delay within the UWB pass band. made to control attenuation pole frequency by adjusting both stub impedance. A center bandwidth of 5-stage BPF are designed based on transmission line model. circuit conditions two poles at either side passband given together controlling them.
Ly-1 B cells have the distinctive property of continuous self-replenishment and, as we shown previously, can be further distinguished from conventional on basis greatly elevated constitutive and inducible production recently described cytokine interleukin 10 (IL-10). To test possibility that IL-10 acts either an autocrine or paracrine growth factor for cells, treated mice continuously birth to 8 wk age with a monoclonal rat IgM antibody specifically neutralizes mouse IL-10. Mice in this way...
Immunocytochemical electron-microscopic observation indicated that ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) and/or its degradation products are localized in small spherical bodies having a diameter of 0.4–1.2 µm naturally senescing leaves wheat (Triticum aestivum L.). These Rubisco-containing (RCBs) were found the cytoplasm and vacuole. RCBs contained another stromal protein, chloroplastic glutamine synthetase, but not thylakoid proteins. Ultrastructural...
An NO-selective electrode was developed as an easily applicable tool for a real-time nitric oxide (NO) measurement. The working (0.2 mm diam) made from Pt/Ir alloy coated with three-layered membrane. counterelectrode carbon fiber. When stable NO donor, S-nitroso-N-acetyl-dl-penicillamine, applied, the current increased in dose-dependent fashion. and calculated concentration showed linear relationship range 0.2 nM (S/N=1) to 1 μM of NO. response 1.14±0.09 s. effects temperature, pH, chemicals...
We have measured Raman spectra of diamond films prepared by a hot-filament method and found that layers on Si substrates are under compressive strain. The degree the strain is to increase with increasing nondiamond component in films. It shown spectroscopy powerful estimate crystalline quality, especially
Abstract Autophagy is an intracellular process facilitating the vacuolar degradation of cytoplasmic components and important for nutrient recycling during starvation. We previously demonstrated that chloroplasts can be partially mobilized to vacuole by autophagy via spherical bodies named Rubisco-containing (RCBs). Although contain approximately 80% total leaf nitrogen represent a major carbon source new growth, relationship between status RCB production remains unclear. examined effects...
Abstract Autophagy is an intracellular process leading to the vacuolar degradation of cytoplasmic components. Autophagic chloroplasts particularly activated in leaves under conditions low sugar availability. Here, we investigated importance autophagy energy availability and growth Arabidopsis (Arabidopsis thaliana). autophagy-deficient (atg) mutants showed reduced short-day conditions. This inhibition was largely relieved continuous light or combined with feeding exogenous sucrose,...
Logical circuits using Esaki diodes, and which are based on a principle similar to parametron (subharmonic oscillator element) circuits, described. Two diodes used in series form basic element called twin, binary digit is represented by the polarity of potential induced at middle point controlled majority input signals applied point. Unilateral transmission information consisting cascaded twins achieved dividing into three groups energizing each group one after another cyclic manner....
Much of the nitrogen in leaves is distributed to chloroplasts, mainly photosynthetic proteins. During leaf senescence, chloroplastic proteins, including Rubisco, are rapidly degraded, and released remobilized reused newly developing tissues. Autophagy facilitates degradation intracellular components for nutrient recycling all eukaryotes, recent studies have revealed critical roles autophagy Rubisco remobilization into seeds Arabidopsis (Arabidopsis thaliana). Here, we examined function...
Plant chloroplasts constantly accumulate damage caused by visible wavelengths of light during photosynthesis. Our previous study revealed that entire photodamaged are subjected to vacuolar digestion through an autophagy process termed chlorophagy; however, how this is induced and executed remained poorly understood. In study, we monitored intracellular induction chlorophagy in Arabidopsis (Arabidopsis thaliana) leaves found mesophyll cells damaged high displayed abnormal with a swollen shape...
Autophagy is an intracellular process leading to vacuolar or lysosomal degradation of cytoplasmic components in eukaryotes. Establishment proper methods monitor autophagy was a key step uncovering its role organisms, such as yeast (Saccharomyces cerevisiae), mammals, and Arabidopsis (Arabidopsis thaliana), which chloroplastic proteins were found be recycled by autophagy. Chloroplast recycling has been predicted function nutrient remobilization for growing organs grain filling cereal crops....
The vacuolar lytic degradation of proteins releases free amino acids that plants can use instead sugars for respiratory energy production. Autophagy is a major cellular process leading to the transport into vacuole degradation. Here, we examine contribution autophagy acid metabolism response sugar starvation in mature leaves Arabidopsis thaliana. During arising from exposure wild-type (WT) darkness, autophagic chloroplast stroma, which contains most leaf, lumen was induced within 2 d. this...
Raman spectra of diamond powders with size less than 2 μm have been measured as a function the particle size. The line was found to become more asymmetric some tailing towards lower shifts, broader, and weaker decreasing observed result can be explained by phonon confinement effect rather strain effect. This work predicts that it is very difficult detect particles ∼50 Å. A broad band, whose intensity becomes stronger size, around 600 cm−1 in μm. We hypothesize band arises from transverse...
Lysates of chloroplasts isolated from wheat (Triticum aestivum L. cv. Aoba) leaves were incubated on ice (pH 5.7) for 0 to 60 min in light (15 μmol quanta m−2 s−1), and degradation the large subunit (LSU) ribulose-l,5-bis-phosphate carboxylase/oxygenase (Rubisco: EC 4.1.1.39) was analyzed by applying immunoblotting with site-specific antibodies against N-terminal, internal, C-terminal amino acid sequences LSU Rubisco. The most dominant product breakdown that which first appear an apparent...
Raman spectra of B ion-implanted glassy carbon (GC) and hydrogenated amorphous (a-C:H) films have been measured as a function polarization direction the scattered light excitation wavelength. bands GC implanted heavily with fluence more than 5\ifmmode\times\else\texttimes\fi{}${10}^{15}$ ions/${\mathrm{cm}}^{2}$ a-C:H show frequency-independent depolarization ratio, suggesting existence ${\mathit{sp}}^{2}$ clusters in these samples. It was found that varied From comparative study films,...