A5040450419- Microfluidic and Capillary Electrophoresis Applications
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Glycosylation and Glycoproteins Research
- Microfluidic and Bio-sensing Technologies
- Electrostatic Discharge in Electronics
- Virus-based gene therapy research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Integrated Circuits and Semiconductor Failure Analysis
- Toxoplasma gondii Research Studies
- Drug Solubulity and Delivery Systems
- Mosquito-borne diseases and control
- Clostridium difficile and Clostridium perfringens research
- Biosensors and Analytical Detection
- Cervical Cancer and HPV Research
- Safe Handling of Antineoplastic Drugs
- Atomic and Subatomic Physics Research
- Advanced Biosensing Techniques and Applications
- Molecular Biology Techniques and Applications
- Insect symbiosis and bacterial influences
- Bacterial Identification and Susceptibility Testing
- T-cell and B-cell Immunology
- PARP inhibition in cancer therapy
Merck & Co., Inc., Rahway, NJ, USA (United States)
2013-2022
United States Military Academy
2013-2022
Institut de Recherche Vaccinale
2015
Krankenhaus Düren
2008
Human cytomegalovirus (HCMV) is the leading cause of congenital viral infection, and developing a prophylactic vaccine high priority to public health. We recently reported replication-defective human with restored pentameric complex glycoprotein H (gH)/gL/pUL128-131 for prevention HCMV infection. While quantity vaccine-induced antibody responses can be measured in neutralization assay, assessing quality such responses, including ability antibodies cross-neutralize field strains HCMV, remains...
Many CE-based technologies such as imaged capillary IEF, CE-SDS, CZE, and MEKC are well established for analyzing proteins, viruses, or other biomolecules polysaccharides. For example, isoelectric focusing (charge-based protein separation) CE-SDS (size-based standard replacement methods in biopharmaceutical industries tedious labor intensive IEF SDS-PAGE methods, respectively. Another important analytical tool characterization is a Western blot, where after size-based separation the proteins...
An in vitro relative potency (IVRP) assay has been developed as an alternative to the mouse used release Merck's human papillomavirus (HPV) vaccine, Gardasil, for early phase clinical trials. The is a classical, vivo assay, which requires 4-6 weeks complete and exhibits variability on order of 40% standard deviation (RSD). IVRP sandwich-type immunoassay that measure antigenicity vaccine product. can be completed three days, approximately 10% RSD does not require sacrifice live animals....
The thermostability of GARDASIL (Merck & Co., Inc, Whitehouse Station, NJ, USA), a developmental vaccine against human papillomavirus (HPV), was evaluated using an enzyme immunoassay, referred to as the in vitro relative potency (IVRP) assay and differential scanning calorimetry (DSC). Gardasil samples were stored at temperatures ranging from 4 42 degrees C tested for IVRP various time points. Extrapolation results indicates is extremely stable. half-life estimated be 130 months or longer up...
An international team that included 20 independent laboratories from biopharmaceutical companies, universities, analytical contract and national authorities in the United States, Europe Asia was formed to evaluate reproducibility of sample preparation analysis N-glycans using capillary electrophoresis 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled glycans with laser induced fluorescence (CE-LIF) detection (16 sites) ultra high-performance liquid chromatography (UHPLC, 12 sites; results...
The N-linked glycosylation in recombinant monoclonal antibodies (mAb) occurs at Asn297 on the Fc region CH2 domain. Glycosylation heterogeneities have been well documented to affect biological activities such as antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent (CDC) through their interaction with Fc-receptors. Hence, it is critical monitor characterize profile a therapeutic protein mAb for product consistency. In one approach, glycans are first released from using an...
Both poly and mono ADP-ribosylation are common posttranslational protein modifications. For example, is involved in DNA repair mechanisms through the (ADP-ribose) polymerase (PARP) family of enzymes. While has been known to trigger cell death exhibited by many bacterial toxins. Because wide role ADP-ribosylation, detection analysis very important for further understanding PARP enzymes molecular leading toxicity presence Here, we describe a novel technique utilizing CE-based Western...
Dengue is known to cause morbidity and mortality worldwide currently there neither available specific therapeutics treat nor a vaccine prevent this disease. Although efforts are being made, development of against disease remains challenging. Hawaii Biotech Inc developed recombinant subunit envelope protein-based all four serotypes produced in Drosophila S2 cells which were transferred over Merck 2010. Each the dengue contains N-terminal 80% amino acids comprising protein (DEN-80E). A Phase 1...
CDTa, an actin ADP-ribosylation transferase, is a binary toxin produced by the bacterium Clostridium difficile which commonly associated with hypervirulent strain present in infections. The mutated form of 4mCDTa, one components tetravalent vaccine residual toxicity activity needs to be monitored for safety reasons. There are several ADP- ribosylation methods employing techniques such as ELISA, manual Western blot, or SDS PAGE, but all these usually time consuming and labor intensive. Here...
In einer prospektiven Studie wurde die In-vitro-Stabilität des prostataspezifischen Antigens (PSA) bei Lagerungstemperatur von -20°C untersucht. Nach Lagerungsdauer 20 bis 42 Monaten (Mittel 32 Monate) war einem Korrelationskoeffizienten r = 0,99 eine mittlere Abnahme der PSA-Konzentration lediglich 0,6% zu verzeichnen. Die Bestimmung 128 Serumproben (Minimum 0,1, Maximum 1.120 ng/ml) erfolgte mit dem äquimolar messenden Tandem®-E Firma Hybritech. Weder noch Art Erkrankung oder Höhe zeigten...
PedvaxHIB® is an effective pediatric vaccine for protecting infants from invasive gram-negative bacterium Haemophilus influenzae type b. It a highly purified capsular polysaccharide, polyribosylribitol phosphate that covalently linked to outer membrane protein complex of Neisseria meningitidis. PRP first derivatized with organic linker, followed by the coupling butadiamine group, and then at end terminal, bromoacetyl group attached conjugation thiolated OMPC. The stability bromide in...