A5072264341- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Carbohydrate Chemistry and Synthesis
- Microfluidic and Capillary Electrophoresis Applications
- Lung Cancer Research Studies
- Iron oxide chemistry and applications
- Genomics and Phylogenetic Studies
- Metastasis and carcinoma case studies
- Nanoparticle-Based Drug Delivery
- Magnetic Properties and Synthesis of Ferrites
- Diagnosis and Treatment of Venous Diseases
- Influenza Virus Research Studies
- Photochromic and Fluorescence Chemistry
- HIV Research and Treatment
- Herpesvirus Infections and Treatments
- Appendicitis Diagnosis and Management
- Electrostatics and Colloid Interactions
- Eating Disorders and Behaviors
- Intestinal Malrotation and Obstruction Disorders
- Hemoglobin structure and function
- Venous Thromboembolism Diagnosis and Management
- Machine Learning in Bioinformatics
- Gastrointestinal Tumor Research and Treatment
- Evolutionary Psychology and Human Behavior
University of Miskolc
2019-2025
University of Debrecen
2013-2024
National Institute for Bioprocessing Research and Training
2016-2017
Hungarian Academy of Sciences
2012
Full automation to enable high throughput N-glycosylation profiling and sequencing with good reproducibility is vital fulfill the contemporary needs of biopharmaceutical industry requirements national regulatory agencies. The most prevalently used glycoanalytical methods capillary electrophoresis hydrophilic interaction liquid chromatography, while very efficient, both necessitate extensive sample preparation cleanup, including glycoprotein capture, N-glycan release, fluorescent...
Background: Subarachnoid hemorrhage is a serious condition caused by ruptured intracranial aneurysms, resulting in severe disability mainly young adults. Cerebral vasospasm one of the most common complication subarachnoid hemorrhage; thus, active prevention key to improve prognosis. The glycosylation proteins critical quality attribute which reportedly altered patients diagnosed with acute ischemic stroke. In this study, we examined N-glycosylation profile serum glycoproteins without...
The signature of human serum IgG glycosylation is critical in the defense against pathogens. Alterations N-glycome were associated with COVID-19 (Coronavirus disease 2019) severity, although knowledge on response to vaccination limited. was analyzed this study post-COVID-19 and post-vaccination patients reveal potential glycosylation-based alterations using hydrophilic interaction liquid chromatography (HILIC-UPLC) fluorescence (FLR) mass-spectrometric (MS) detection. antibodies purified...
In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support machine-estimated recursive elimination or SVM-RFE) to identify potential glycan alterations Parkinson’s disease. Specific focus was placed on use neutral coated capillaries, by dynamic coating strategy, ensure stable repeatable separations without need non-mass spectrometry (MS) friendly additives...
The combination of the sonochemical activation Ni(NO3)2 and Co(NO3)2 in presence Fe(NO3)3 polyethylene glycol consecutive heat treatment formed metal hydroxides offers a cheap efficient method for preparation nickel ferrite cobalt magnetic nanoparticles, which can be successfully applied selective capture fluorescently derivatized N-glycans from human serum. XRD measurement revealed that, besides phase, oxides also form during treatment. amount simple well controlled by temperature...
An international team that included 20 independent laboratories from biopharmaceutical companies, universities, analytical contract and national authorities in the United States, Europe Asia was formed to evaluate reproducibility of sample preparation analysis N-glycans using capillary electrophoresis 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled glycans with laser induced fluorescence (CE-LIF) detection (16 sites) ultra high-performance liquid chromatography (UHPLC, 12 sites; results...
A CE‐based method was introduced to compare the N ‐glycosylation profile of haptoglobin in normal and pathologic conditions. To assess biomarker potential glycosylation changes various lung diseases, isolated from plasma samples healthy, pneumonia, chronic obstructive pulmonary disease, cancer patients by means two haptoglobin‐specific monoclonal antibodies. Haptoglobin ‐glycans were then enzymatically released, fluorescently labeled, profiled CE. Disease‐associated core antennary...
A simplified, fast, and effective production method has been developed for the synthesis of manganese ferrite (MnFe2O4) magnetic nanoparticles (MNPs). In addition to wide applicability MnFe2O4 MNPs, this work also reports their application in DNA isolation first time. An ultrasonic-cavitation-assisted combustion was applied MNPs at different furnace temperatures (573 K, 623 673 773 K) optimize particles' properties. It shown that synthesized 573 K consist a spinel phase only with adequate...
Objective. Tocilizumab, a humanized anti-interleukin-6 receptor monoclonal antibody, has recently been approved as biological therapy for rheumatoid arthritis (RA) and other diseases. It is not known if there are characteristic changes in gene expression immunoglobulin G glycosylation during or response to treatment. Methods. Global profiles from peripheral blood mononuclear cells of 13 patients with RA active disease at Week 0 (baseline) 4 following treatment were obtained together clinical...
Site-specific glycosylation of Cetuximab was characterized in this study using multiple fractionation methods and capillary electrophoresis coupled to mass spectrometry (CE-MS) based glycomics. IdeS digested with subsequent reduction fractionated reversed-phase chromatography resulting 3 fragments; Fd, Lc Fc/2. Glycan release the different fragments performed 18O enriched water providing possible quantification site occupancy. 2-AA labelled glycan structures were annotated by CE-MS profiling...
The human immunodeficiency virus (HIV) envelope glycoprotein (Env) is the primary antigenic feature on surface of and key importance in HIV vaccinology. Vaccine trials with gp120 subunit Env are ongoing, recent RV144 trial showing moderate efficacy. densely covered N ‐linked glycans that thought to help evade host's humoral immune response. To assess how global glycosylation patterns vary between constructs, glycan profiles several gp120s were examined by CE LIF detection MALDI‐MS. found be...
Sonochemistry in combination with a combustion procedure was used to prepare superparamagnetic iron oxide nanoparticles. Two different precursors, ferrocene and iron(II)-acetate were dispersed polyethylene-glycol (PEG) molecular weight (200–1000) by using high efficiency ultrasonic technique. The sonochemical treatment initiated the precipitation of iron-hydroxide particles, then PEG based colloids burnt create Three phases observed samples, magnetite (Fe3O4), hematite (α-Fe2O3), maghemite...
Prediction of responsiveness in biological therapies is an important and challenging issue different diseases. Analyzing glycosylation pattern changes key serum glycoproteins one the possible avenues to follow disease remission. The aim this study was investigate IgG glycoforms Crohn's (CD) rheumatoid arthritis patients response antitumor necrosis factor alpha (anti-TNF-α) treatment. isolated from patient samples using Protein A affinity pull-down, followed by release N-glycans with...
In the first part of this publication, results from an international study evaluating precision (i.e., repeatability and reproducibility) N-glycosylation analysis using capillary electrophoresis APTS-labeled N-glycans were presented. The corresponding ultra-high performance liquid chromatography (UHPLC) with fluorescence detection are presented here 12 participating sites. All participants used same lot samples, reagents, columns to perform assays. Elution time, peak area percent values...
A novel glycoanalytical approach was developed in this study for the purification of fluorescently derivatized N-glycans. Polyethylene glycol (PEG) modified iron-nanoparticles were synthetized by combination sonochemical treatment and combustion method. The prepared nanomaterials applied a systematic clean-up optimization to maximize efficiency 2-AA labelled glycans. PEG 1000 iron-oxalate found be most effective selective enrichment serum N-glycans providing high reproducibility. Different...
The identification of patients with different brain tumors is solely built on imaging diagnostics, indicating the need for novel methods to facilitate disease recognition. Glycosylation a chemical modification proteins, reportedly altered in several inflammatory and malignant diseases, providing potential alternative route detection. In this paper, we report quantitative analysis serum N-glycosylation diagnosed primary metastatic tumors. PNGase-F-digested procainamide-labeled glycans were...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has caused a global concern since its outbreak in 2019, with one of the main solutions being vaccination. Altered glycosylation been described patients after SARS-CoV-2 infection, while effect vaccination on serum glycoproteins remained unexplored. In this study, total was analyzed infection and/or mRNA order to identify potential glycosylation-based alterations. Enzyme-linked immunosorbent assay applied post-COVID-19...
Aromatic isocyanides have gained a lot of attention lately as promising antifungal and anticancer drugs, well high-performance fluorescent analytical probes for the detection toxic metals, such mercury, even in vivo. Since this topic is relatively new aromatic possess unique photophysical properties, understanding structure-behavior relationships preparation novel potentially biologically active derivatives are paramount importance. Here, we report characterization 1,5-diisocyanonaphthalene...
The identification of pediatric appendicitis is challenging due to the lack specific markers thereby several factors are included in diagnostic process such as abdominal pain, ultrasonography and altered laboratory parameters (C reactive protein, absolute neutrophil cell number white blood number). glycosylation pattern serum N-glycome was analyzed this study 38 controls 40 patients with appendicitis. glycans were released by enzymatic deglycosylation followed fluorescent labeling...