A5025834611- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Genetic Associations and Epidemiology
- Toxoplasma gondii Research Studies
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Parvovirus B19 Infection Studies
- Escherichia coli research studies
- Respiratory viral infections research
- Virus-based gene therapy research
- Genetic Mapping and Diversity in Plants and Animals
- Biosensors and Analytical Detection
- SARS-CoV-2 and COVID-19 Research
- Genetic and phenotypic traits in livestock
- Viral Infections and Immunology Research
- Mitochondrial Function and Pathology
- Systemic Lupus Erythematosus Research
- Mosquito-borne diseases and control
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Genetics and Neurodevelopmental Disorders
- Remote Sensing and Land Use
- Inflammatory Bowel Disease
- Bioinformatics and Genomic Networks
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
Merck & Co., Inc., Rahway, NJ, USA (United States)
2016-2024
Xiamen University
2023-2024
Jinan University
2024
United States Military Academy
2011-2023
Huaqiao University
2023
Central South University
2023
Northeastern University
2023
Brown Foundation
2023
The University of Texas Health Science Center at Houston
2023
University of Science and Technology of China
2023
ABSTRACT Human cytomegalovirus (HCMV), a member of the herpesvirus family, is large complex enveloped virus composed both viral and cellular gene products. While sequence HCMV genome has been known for over decade, full set proteins that compose virion are unknown. To approach this problem we have utilized gel-free two-dimensional capillary liquid chromatography-tandem mass spectrometry (MS/MS) Fourier transform ion cyclotron resonance MS to identify determine relative abundances in purified...
Human cytomegalovirus replicates in many different cell types, including epithelial cells, endothelial and fibroblasts. However, laboratory strains of the virus, which were developed as attenuated vaccine candidates by serial passage fibroblasts, have lost ability to infect cells. Their growth is restricted primarily due mutations UL131-UL128 locus. We now demonstrate that two products this locus, pUL130 pUL128, form a complex with gH gL, but not gO. The AD169 strain, lacks functional UL131...
Epithelial cells are one of the prominent cell types infected by human cytomegalovirus (HCMV) within its host. However, many cultured epithelial cells, such as ARPE-19 retinal pigmented poorly laboratory-adapted strains in culture, and little is known about viral factors that determine HCMV tropism. In this report, we demonstrate UL131 open reading frame (ORF), likely entire UL131-128 locus, required for efficient infection cells. Repair mutated gene AD169 laboratory strain restored ability...
The RSV Fusion (F) protein is a target for neutralizing antibody responses and focus vaccine discovery; however, the process of entry requires F to adopt metastable prefusion form transition more stable postfusion form, which displays less potent epitopes. mRNA vaccines encode antigens that are translated by host cells following vaccination, may allow conformational transitions similar those observed during natural infection occur. Here we evaluate panel chemically modified expressing...
ABSTRACT Canine parvovirus (CPV) enters and infects cells by a dynamin-dependent, clathrin-mediated endocytic pathway, viral capsids colocalize with transferrin in perinuclear vesicles of shortly after entry (J. S. L. Parker C. R. Parrish, J. Virol. 74:1919–1930, 2000). Here we report that CPV feline panleukopenia virus (FPV), closely related parvovirus, bind to the human receptors (TfRs) use these enter infect cells. Capsids did not detectably or quail QT35 Chinese hamster ovary (CHO)...
Use of 3-hydroxyl-3-methylglutaryl-3 coenzyme A reductase (HMGCR) inhibitors, or statins, reduces cardiovascular disease risk by lowering plasma low-density lipoprotein cholesterol (LDL-C) concentrations. However, LDL-C response is variable and influenced many factors, including racial ancestry, with attenuated in blacks compared whites. We hypothesized that single nucleotide polymorphisms the gene encoding HMGCR, a rate-limiting enzyme synthesis direct enzymatic target contribute to...
Significance Congenital human cytomegalovirus (HCMV) infection is an important cause of newborn disability, and developing a vaccine against congenital HCMV top priority. However, despite decades efforts, remains elusive. Previous vaccines lacked antigen called pentameric glycoprotein H (gH) complex, essential for the virus to infect epithelial/endothelial cells, these induced poor neutralizing antibodies. To support unique concept featuring gH we established 45 mAbs from rabbit immunized...
A new replication-defective human cytomegalovirus vaccine shows promise in protecting against this dangerous virus that can cause severe neurodevelopmental defects babies.
Abstract Respiratory syncytial virus (RSV) infection is the leading cause of hospitalization and infant mortality under six months age worldwide; therefore, prevention RSV in all infants represents a significant unmet medical need. Here we report isolation potent broadly neutralizing monoclonal antibody derived from human memory B-cell. This antibody, RB1, equipotent on A B subtypes, potently neutralizes diverse panel clinical isolates vitro demonstrates vivo protection. It binds to highly...
Significance The urgency of curbing the COVID-19 pandemic has motivated many investigators to pivot their research understand basic biology SARS-CoV-2 and search for new pharmaceutical compounds potential treatment. However, most studies require biosafety level 3 facilities, which are in high demand, costly, difficult access. To overcome these limitations, we engineered a replicon, is modified virus subgenome capable self-replicating without producing infectious virus, allowing viral...
ABSTRACT The unique N-terminal region of the parvovirus VP1 capsid protein is required for infectivity by capsids but not assembly. N terminus contains a number groups basic amino acids which resemble classical nuclear localization sequences, including conserved sequence near comprised four acids, in peptide can act to transport other proteins into cell nucleus. Testing with monoclonal antibody recognizing residues 2 13 (anti-VP1-2-13) and rabbit polyclonal serum against entire showed that...
The IL-23 receptor (IL-23R) has been found to be associated with small bowel Crohn's disease (CD) in a whole genome association study. Specifically, the rare allele of R381Q single nucleotide polymorphism (SNP) conferred protection against CD. It is unknown whether IL-23R IBD children. aim was examine susceptibility pediatric patients. DNA collected from 609 subjects (151 CD and 52 ulcerative colitis [UC] trios). Trios were genotyped for SNP gene SNP8, SNP12, SNP13, CARD15 using Taqman....
ABSTRACT Cyclin-dependent kinases (cdk's) have recently been suggested to regulate human immunodeficiency virus type 1 (HIV-1) transcription. Previously, we shown that expression of one cdk inhibitor, p21/Waf1, is abrogated in HIV-1 latently infected cells. Based on this result, investigated the transcription presence chemical drugs specifically inhibited activity and functionally mimicked p21/Waf1 activity. production virally integrated lymphocytic monocytic cell lines, such as ACH 2 , 8E5,...
The human cytomegalovirus pUL21.5 protein is a small, secreted glycoprotein whose mRNA packaged into virions. We demonstrate that soluble CC chemokine receptor functions as decoy to modulate the host immune response infection. In contrast other viral chemokine-binding proteins, which interact promiscuously with multiple chemokines, selectively binds RANTES (regulated upon activation, normal T cell expressed and secreted) high affinity. By binding RANTES, blocks interaction its cellular...
Nine neurodegenerative diseases, collectively referred to as polyglutamine (polyQ) are caused by expansion of a coding CAG DNA trinucleotide repeat. PolyQ diseases show strong inverse correlation between repeat length and age disease onset (AO). Despite this, individuals with identical alleles can have highly variable indicating that other factors also influence AO. We examined AO in 148 57 sibships from the SCA2 founder population Cuba. The mutant allele explained 57% variance. To estimate...
A class of anti-virulence compounds, the salicylidene acylhydrazides, has been widely reported to block function type three secretion system several Gram-negative pathogens by a previously unknown mechanism. In this work we provide first identification bacterial proteins that are targeted group compounds. We evidence their mode action is likely result from synergistic effect arising perturbation conserved proteins. also examine contribution selected target pathogenicity Yersinia...
Human cytomegalovirus (HCMV) is the leading cause of congenital viral infection, and developing a prophylactic vaccine high priority to public health. We recently reported replication-defective human with restored pentameric complex glycoprotein H (gH)/gL/pUL128-131 for prevention HCMV infection. While quantity vaccine-induced antibody responses can be measured in neutralization assay, assessing quality such responses, including ability antibodies cross-neutralize field strains HCMV, remains...
A conditionally replication-defective human cytomegalovirus (CMV) vaccine (V160) derived from AD169 and genetically engineered to express CMV pentameric complex (gH/gL/pUL128/pUL130/pUL131) was developed evaluated for phase 1 safety immunogenicity in CMV-seronegative CMV-seropositive adults.Subjects received 3 doses of V160 or placebo on day 1, month 6. Four dose levels, formulated with without aluminum phosphate adjuvant, were evaluated. Injection-site systemic adverse events (AEs) viral...