A5101779611- MicroRNA in disease regulation
- Lung Cancer Treatments and Mutations
- Gastrointestinal Tumor Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Circular RNAs in diseases
- Gastric Cancer Management and Outcomes
- Gastrointestinal disorders and treatments
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- Radiomics and Machine Learning in Medical Imaging
- Ferroptosis and cancer prognosis
- CAR-T cell therapy research
- Hematopoietic Stem Cell Transplantation
- Cytomegalovirus and herpesvirus research
- T-cell and B-cell Immunology
- Nutrition and Health in Aging
- Immunotherapy and Immune Responses
- Drug Transport and Resistance Mechanisms
- Glycosylation and Glycoproteins Research
- Cancer Mechanisms and Therapy
- RNA modifications and cancer
- HER2/EGFR in Cancer Research
Xiamen University
2011-2025
First Affiliated Hospital Zhejiang University
2020-2025
Dalian Medical University
2024
Chinese Academy of Sciences
2022-2024
Changhai Hospital
2024
Second Military Medical University
2024
People's Hospital of Yangzhong
2011-2024
Zhejiang University
2019-2024
West China Hospital of Sichuan University
2009-2023
Sichuan University
2009-2023
Abstract microRNAs are endogenous small noncoding RNAs that regulate gene expression negatively at posttranscriptional level. This latest addition to the complex regulatory circuitry revolutionizes our way understanding physiological and pathological processes in human body. Here we investigated possible role of development multidrug resistance (MDR) gastric cancer cells. microRNA profiling revealed a limited set with altered multidrug‐ resistant cell line SGC7901/VCR compared its parental...
Abstract Immunogenic cell death (ICD) converts dying cancer cells into a therapeutic vaccine and stimulates antitumor immune responses. Here we unravel the results of an unbiased screen identifying high-dose (10 µM) crizotinib as ICD-inducing tyrosine kinase inhibitor that has exceptional antineoplastic activity when combined with non-ICD inducing chemotherapeutics like cisplatin. The combination cisplatin induces ICD in non-small lung carcinoma (NSCLC) effectively controls growth distinct...
Studies have been shown that miR-125a plays an important role in carcinogenesis, however, the of hepatocellular carcinoma (HCC) remains elusive.Real time-PCR (qRT-PCR) was performed to test significance HCC. Ectopic expression used influences on proliferation and metastasis HCC cells vitro vivo. Predicted target genes were determined by dual-luciferase reporting, qRT-PCR, western blot (WB) analyses. Then immunohistochemical staining (IHC) detect genes, correlations prognostic values its also...
Background Hypoxia-induced renal tubular cell epithelial–mesenchymal transition (EMT) is an important event leading to fibrosis. MicroRNAs (miRNAs) are small non-coding RNA molecules that bind their mRNA targets, thereby translational repression. The role of miRNA in hypoxia-induced EMT largely unknown. Methodology/Principal Findings profiling was performed for the identification differentially expressed miRNAs HK-2 cells under normal and low oxygen, results were then verified by...
Significance Congenital human cytomegalovirus (HCMV) infection is an important cause of newborn disability, and developing a vaccine against congenital HCMV top priority. However, despite decades efforts, remains elusive. Previous vaccines lacked antigen called pentameric glycoprotein H (gH) complex, essential for the virus to infect epithelial/endothelial cells, these induced poor neutralizing antibodies. To support unique concept featuring gH we established 45 mAbs from rabbit immunized...
// Qiong Wu 1,* , Zhiping Yang Lin Xia Yongzhan Nie 1 Kaichun Yongquan Shi and Daiming Fan State Key Laboratory of Cancer Biology Xijing Hospital Digestive Diseases, Hospital, Fourth Military Medical University, Xi’an, China * These authors contributed equally to this work Correspondence: Fan, email: Shi, Keywords : MiR-129-5p, Gastric cancer, Drug resistance, ABC transporters Received April 28, 2014 Accepted October 18, Published Abstract Recent studies have reported that...
Abstract Objectives Although targeted therapy has revolutionized the treatment of gastrointestinal stromal tumours (GIST), it is almost never curative in GIST, and resistance commonly develops. One potential strategy to combine with immunotherapy. Materials methods We first studied Programmed cell death 1 ligand (PD‐L1) expression tumour‐infiltrating T cells (TILs) GIST. IFN‐γ was used induce upregulation PD‐L1 GIST‐882 cells, a well‐known GIST line. CD8+ T‐cell apoptosis determined by flow...
Hypoxia is an important microenvironmental factor in the development of renal fibrosis; however, underlying mechanisms are not well elucidated. Here we show that hypoxia induces Bmi1 mRNA and protein expression human tubular epithelial cells. We further demonstrate might be directly regulated by hypoxia-inducible factor-1a (HIF-1a) under low oxygen. Moreover, chromatin immunoprecipitation reporter gene assay studies reveal cooperative transactivation HIF-1α Twist. Enforced...
Triple-negative breast cancer (TNBC) is well known for its strong invasiveness, rapid recurrence and poor prognosis. Immunotherapy, including chimeric antigen receptor-modified T (CAR-T) cells, has emerged as a promising tool to treat TNBC. The identification of specific target tumor the design an effective CAR are among many challenges CAR-T therapy.We reported that epidermal growth factor receptor (EGFR) highly expressed in TNBC consequently designed optimal third generation targeting...
Abstract EGFR-targeted chimeric antigen receptor (CAR) T cells are potent and specific in suppressing the growth of triple-negative breast cancer (TNBC) vitro vivo. However, this study, a subset mice soon acquired resistance, which limits potential use EGFR CAR cells. We aimed to find way overcome observed resistance. Transcriptomic analysis results revealed that T-cell treatment induced set immunosuppressive genes, presumably through IFNγ signaling, T-cell–resistant TNBC tumors. The...
Abstract Estrogen and estrogen receptor alpha (ERα)-induced gene transcription is tightly associated with ERα-positive breast carcinogenesis. ERα-occupied enhancers, particularly super-enhancers, have been suggested to play a vital role in regulating such transcriptional events. However, the landscape of super-enhancers (ERSEs) as well key ERα-induced target genes ERSEs remain be fully characterized. Here, we defined cancer cell lines, demonstrated that bromodomain protein BRD4 master...
To investigate the incidence of incidental gastrointestinal stromal tumor (GIST) and its etiopathogenesis.From January 1, 2000 to December 31, 2007, 13804 cases epithelial malignant (EMT) 521 pancreatic adenocarcinoma (PAC) were successfully treated with surgery at Department General Surgery Thoracic Surgery, West China Hospital, Sichuan University, China. The clinical pathologic data 311 primary GIST, including 257 GIST 54 analyzed.Of patients, had accounting for 17.4%. Of these tumors, 27...
Glioblastoma (GBM) is the deadliest brain malignancy without effective treatments. Here, we reported that epidermal growth factor receptor-targeted chimeric antigen receptor T cells (EGFR CAR-T) were in suppressing of GBM vitro and xenografts derived from cell lines patients mice. However, mice soon acquired resistance to EGFR CAR-T treatment, limiting its potential use clinic. To find ways improve efficacy cells, performed genomics transcriptomics analysis for incubated with found a large...
Background: Complete tumor removal is critical for achieving a good prognosis in patients but remains challenging surgeons. Near-infrared fluorescence-guided surgery (NIRFGS) enables surgeons to accurately localize tumors real time and facilitates accurate resection. Indocyanine green (ICG) has been approved by the U.S. Food Drug Administration National Medical Products many years. Although application of ICG progressed variety surgeries, there are inherent limitations ICG, including poor...
Herein, we firstly reported a series of biphenyl compounds bearing hydroxamic acid moiety as PD-L1/class I HDACs dual inhibitors. Among them, compound 14 displayed the strongest inhibitory activity in vitro against HDAC2 and HDAC3 with IC50 values 27.98 nM 14.47 nM, had an value 88.10 for PD-1/PD-L1 interaction. Importantly, could upregulate expression PD-L1 CXCL10 low-expression cancer cell line (MCF-7), highlighting potential to enhance efficacy by recruiting T-cell infiltration into TME...