A5060479152- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Viral gastroenteritis research and epidemiology
- Antimicrobial Resistance in Staphylococcus
- Trace Elements in Health
- Genomics and Phylogenetic Studies
- Vibrio bacteria research studies
- RNA modifications and cancer
- Antibiotic Resistance in Bacteria
- Gut microbiota and health
- Mycobacterium research and diagnosis
- Cancer Research and Treatments
- Salmonella and Campylobacter epidemiology
- Microbial Metabolism and Applications
- Bacterial biofilms and quorum sensing
- Enzyme-mediated dye degradation
- RNA Research and Splicing
- Endoplasmic Reticulum Stress and Disease
- Yersinia bacterium, plague, ectoparasites research
- Molecular Biology Techniques and Applications
- Enterobacteriaceae and Cronobacter Research
- Hemoglobin structure and function
- Streptococcal Infections and Treatments
UNSW Sydney
2016-2024
University of Edinburgh
2008-2020
Roslin Institute
2008-2020
The University of Melbourne
2015-2018
Peter Doherty Institute
2015-2018
Wellcome Centre for Cell Biology
2014-2018
The University of Queensland
2005-2010
ABSTRACT Staphylococcus aureus is a prominent global nosocomial and community-acquired bacterial pathogen. A strong restriction barrier presents major hurdle for the introduction of recombinant DNA into clinical isolates S. . Here, we describe construction characterization IMXXB series Escherichia coli strains that mimic type I adenine methylation profiles clonal complexes 1, 8, 30, ST93. The enable direct, high-efficiency transformation streamlined genetic manipulation lineages. IMPORTANCE...
Highlights•Transcriptome-wide map of Hfq binding reveals targeting rules•Many sRNAs identified in pathogenicity islands•Pathogenicity-associated anti-sRNAs antagonize encoded core genome•Anti-sRNAs alter cell metabolism as part niche colonizationSummaryIn bacteria, is a RNA chaperone that catalyzes the interaction mRNAs with regulatory small RNAs (sRNAs). To determine vivo sequence requirements for interactions, and to study riboregulation bacterial pathogen, was UV crosslinked...
A new variant of Streptococcus pyogenes serotype M1 (designated 'M1UK') has been reported in the United Kingdom, linked with seasonal scarlet fever surges, marked increase invasive infections, and exhibiting enhanced expression superantigen SpeA. The progenitor S. 'M1global' M1UK clones can be differentiated by 27 SNPs 4 indels, yet mechanism for speA upregulation is unknown. Here we investigate previously unappreciated expansion Australia, now isolated from majority serious infections...
Abstract RNA sequencing studies have identified hundreds of non‐coding s in bacteria, including regulatory small ( sRNA ). However, our understanding function has lagged behind their identification due to a lack tools for the high‐throughput analysis – interactions bacteria. Here we demonstrate that vivo mRNA duplexes can be recovered using UV ‐crosslinking, ligation and hybrids CLASH Many recruit endoribonuclease, RN ase E, facilitate processing s. We were able recover base‐paired...
Enterohaemorrhagic Escherichia coli O157 : H7 is a bacterial pathogen that can cause haemorrhagic colitis and haemolytic uremic syndrome. In the primary reservoir host, cattle, terminal rectum principal site of E. colonization. this study, bovine rectal epithelial cells were used to examine role flagella in adherence. Binding fliC(H7) mutant strain epithelium was significantly reduced as binding flagellated wild-type following incubation with H7-specific antibodies. Complementation restored...
The major human pathogen Mycobacterium tuberculosis can survive in the host organism for decades without causing symptoms. A large cohort of Toxin–Antitoxin (TA) modules contribute to this persistence. Of these, 48 TA belong vapBC (virulence associated protein) gene family. VapC toxins are PIN domain endonucleases that, enterobacteria, inhibit translation by site-specific cleavage initiator tRNA. In contrast, VapC20 M. inhibits universally conserved Sarcin-Ricin loop (SRL) 23S rRNA. Here we...
By shaping gene expression profiles, small RNAs (sRNAs) enable bacteria to efficiently adapt changes in their environment. To better understand how Escherichia coli acclimatizes nutrient availability, we performed UV cross-linking, ligation and sequencing of hybrids (CLASH) uncover Hfq-associated RNA-RNA interactions at specific growth stages. We demonstrate that Hfq CLASH robustly captures bona fide interactions. identified hundreds novel sRNA base-pairing interactions, including many...
ABSTRACT Salmonella enterica serovar Typhimurium possesses a multi-copper-ion oxidase (multicopper oxidase), CueO (also known as CuiD), periplasmic enzyme to be required for resistance copper ions. from S . was expressed recombinant protein in Escherichia coli , and the purified exhibited high cuprous activity. We have characterized an cueO mutant confirmed that it is more sensitive Using murine model of infection, observed significantly attenuated, indicated by reduced recovery bacteria...
A class of anti-virulence compounds, the salicylidene acylhydrazides, has been widely reported to block function type three secretion system several Gram-negative pathogens by a previously unknown mechanism. In this work we provide first identification bacterial proteins that are targeted group compounds. We evidence their mode action is likely result from synergistic effect arising perturbation conserved proteins. also examine contribution selected target pathogenicity Yersinia...
ABSTRACT Escherichia coli is the primary cause of urinary tract infection (UTI) in developed world. The major factors associated with virulence uropathogenic E. (UPEC) are fimbrial adhesins, which mediate specific attachment to host receptors and trigger innate responses. Another group adhesins represented by autotransporter (AT) subgroup proteins. In this study, we identified a new AT-encoding gene, termed upaH , present 6.5-kb unannotated intergenic region genome prototypic UPEC strain...
Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterial pathogen responsible for significant human morbidity and mortality. Post-transcriptional regulation by small RNAs (sRNAs) has emerged as an important mechanism controlling virulence. However, the functionality of majority sRNAs during infection unknown. To address this, we performed UV cross-linking, ligation, sequencing hybrids (CLASH) in MRSA to identify sRNA-RNA interactions under conditions that mimic host environment....
Abstract Treatment of methicillin-resistant Staphylococcus aureus infections is dependent on the efficacy last-line antibiotics including vancomycin. failure commonly linked to isolates with intermediate vancomycin resistance (termed VISA). These have accumulated point mutations that collectively reduce sensitivity, often by thickening cell wall. Changes in regulatory small RNA expression been correlated antibiotic stress VISA however functions most regulators unknown. Here we capture...
Recent work has highlighted a number of compounds that target bacterial virulence by affecting gene regulation. In this work, we show small-molecule inhibitors affect the expression type III secretion system (T3SS) Escherichia coli O157:H7 in liquid culture and when bacterium is attached to bovine epithelial cells. Inhibition T3SS resulted reduction capacity bacteria form attaching effacing lesions. Our results there marked variation abilities four structurally related inhibit panel...
Lytic or lysogenic infections by bacteriophages drive the evolution of enteric bacteria. Enterohemorrhagic Escherichia coli (EHEC) have recently emerged as a significant zoonotic infection humans with main serotypes carried ruminants. Typical EHEC strains are defined expression type III secretion (T3S) system, production Shiga toxins (Stx) and association specific clinical symptoms. The genes for Stx present on lambdoid integrated into E. genome. Phage (PT) 21/28 is most prevalent strain...
Summary Type III secretion (T3S) plays a pivotal role in the colonization of ruminant hosts by Enterohemorrhagic Escherichia coli (EHEC). The T3S system translocates effector proteins into host cells to promote bacterial attachment and persistence. repertoire variation prophage regions underpins differences pathogenesis epidemiology EHEC strains. In this study, we have used collection deletions cryptic prophages O157 O‐islands screen for novel regulators T3S. Using approach identified family...
Summary This study has identified horizontally acquired genomic regions of enterohaemorrhagic Escherichia coli O157:H7 that regulate expression the type III secretion (T3S) system encoded by locus enterocyte effacement (LEE). Deletion O‐island 51, a 14.93 kb cryptic prophage (CP‐933C), resulted in reduction LEE and T3S. The deletion also had reduced capacity to attach epithelial cells significantly E. O157 excretion levels from sheep. Further characterization 51 novel positive regulator LEE,...
ABSTRACT The EspF protein is secreted by the type III secretion system of enteropathogenic and enterohemorrhagic Escherichia coli (EPEC EHEC, respectively). sequences differ between EHEC O157:H7, O26:H11, EPEC O127:H6 in terms number SH3-binding polyproline-rich repeats specific residues these regions, as well amino domain involved cellular localization. O127 important for inhibition phagocytosis also limits translocation through antigen-sampling cells (M cells). has been shown to have...
Enterohemorrhagic Escherichia coli is a significant human pathogen that causes disease ranging from hemorrhagic colitis to hemolytic uremic syndrome. The latter can lead potentially fatal renal failure and caused by the release of Shiga toxins are encoded within lambdoid bacteriophages. late transcript phage regulated antitermination PR' promoter during lytic induction phage. During lysogeny, prematurely terminated at tR' immediately downstream PR', generating short RNA byproduct regulation....