A5082840407- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- Biochemical and Structural Characterization
- Bacterial Identification and Susceptibility Testing
- Toxin Mechanisms and Immunotoxins
- Protease and Inhibitor Mechanisms
- Infective Endocarditis Diagnosis and Management
- Glutathione Transferases and Polymorphisms
- Phytoplasmas and Hemiptera pathogens
- Genomics, phytochemicals, and oxidative stress
- Antimicrobial Resistance in Staphylococcus
- Peptidase Inhibition and Analysis
- Advanced Electron Microscopy Techniques and Applications
- Signaling Pathways in Disease
- Selenium in Biological Systems
- RNA and protein synthesis mechanisms
- Endometriosis Research and Treatment
- Blood Coagulation and Thrombosis Mechanisms
- Blood properties and coagulation
- Botanical Research and Chemistry
University of Notre Dame
2019-2024
Obafemi Awolowo University
2019
Cellular plasminogen (Pg) receptors (PgRs) are utilized to recruit Pg; stimulate its activation the serine protease, plasmin (Pm); and sterically protect surface Pm from inactivation by host inhibitors. One such PgR is moonlighting enzyme, enolase, some of which leaves cytoplasm resides at cell potentially function as a PgR. Since microbes employ conscription Pg PgRs one virulence mechanism, we explored structural basis ability Streptococcus pyogenes enolase (Sen) in this manner. Employing...
Accelerated activation of the human plasminogen zymogen (hPg) to two-chain active plasmin (hPm) is achieved following conformational changes induced by ligand-binding at lysine-binding sites (LBSs) in four five hPg kringle domains. In this manner, pattern D skin-trophic strains Group A streptococci (GAS), through expression surface plasminogen-binding M-protein (PAM), immobilize hPg, thereby enabling rapid GAS-secreted streptokinase (SK). Consequently, GAS enhances virulence digesting...
Invasive outcomes of Group A
Virulent strains of Streptococcus pyogenes (gram-positive group A [GAS]) recruit host single-chain human plasminogen (hPg) to the cell surface—where in case Pattern D GAS, hPg binds directly cells through a surface receptor, plasminogen-binding streptococcal M-protein (PAM). The coinherited GAS-secreted streptokinase (SK2b) then accelerates cleavage at R561-V562 peptide bond, resulting disulfide-linked two-chain protease, plasmin (hPm). hPm localizes on bacterial surface, assisting...
The proteolytic activity of human plasmin (hPm) is utilized by various cells to provide a surface protease that increases the potential migrate and disseminate. Skin-trophic Pattern D strains Streptococcus pyogenes (GAS), e.g., GAS isolate AP53, contain M-protein (PAM) directly strongly interacts (Kd ~ 1 nM) with host plasminogen (hPg), after which it activated hPm specific coinherited bacterial activator, streptokinase (SK2b), or activators. Another ubiquitous class hPg binding proteins on...
Group A Streptococcus pyogenes (GAS) is a human-specific pathogen that produces many surface factors, including its signature M protein, contribute to pathogenicity. proteins undergo specific membrane localization and anchoring the cell wall via transpeptidase sortase A. Herein, we explored role of function on protein localization, architecture, function, employing, skin-tropic GAS isolate, AP53, which expresses human plasminogen (hPg)-binding (PAM) Protein. We showed PAM anchored in...
Abstract The direct binding of human plasminogen (hPg), via its kringle‐2 domain (K2 hPg ), to streptococcal M‐protein (PAM), largely contributes the pathogenesis Pattern D Group A Streptococcus pyogenes (GAS). However, mechanism complex formation is unknown. In a system consisting Class II PAM from GAS isolate NS88.2 (PAM with one K2 a‐repeat in A‐domain, we employed biophysical techniques analyze /PAM interaction. We show that apo‐PAM coiled‐coil homodimer (M.Wt. ~80 kDa) at 4°C–25°C, and...
Human plasminogen (PLG), the zymogen of fibrinolytic protease, plasmin, is a polymorphic protein with two widely distributed codominant alleles, PLG/Asp
Abstract Bulinus globosus is a freshwater snail that serves as intermediate host for Schistosoma haematobium and known to be drought-tolerant. A previous report on the alteration in status of glutathione S -transferase (GST) B. during aestivation recovery, suggested GST might involved adaptation organism drought. Therefore, present study aims characterize isoforms are displayed snails under drought stress, sampled dry season, those rainy season. Our data show hepatopancreas contained highest...
Trafficking of M-protein (Mprt) from the cytosol Group A Streptococcus pyogenes (GAS) occurs via Sec translocase membrane channels that associate with Sortase (SrtA), an enzyme catalyzes cleavage Mprt at proximal C-terminal [-LPST
Abstract The surface of Streptococcus pyogenes (GAS) is studded with virulence determinants, the most abundant being characteristic M-protein used to serotype various strains bacterium. There are >250 GAS serotypically distinguished by their M-proteins. Major pathogenic mechanisms require that this microorganism hijacks host components for survival, many which involved in hemostasis. One these processes involves binding human plasminogen (hPg) an receptor (PAM). When bound PAM, hPg...
Bulinus globosus is a freshwater snail that serves as intermediate host for Schistosoma haematobium and is known to be drought-tolerant. A previous report on the alteration in status of glutathione S-transferase (GST) B. globosus during aestivation recovery, suggested GST might be involved adaptation organism drought. Therefore, present study aims characterize isoforms are displayed snails under drought stress, sampled dry season, those under the rainy season. Our data show that the...
ABSTRACT Cellular plasminogen (Pg) receptors (PgR) are utilized to recruit Pg, stimulate its activation the serine protease, plasmin (Pm), and sterically protect generated Pm from inactivation by natural host inhibitors. The net result is that cells contain a stable proteolytic surface used for biological mechanisms involved in cell migration. One such PgR moonlighting enzyme, enolase, some of which leaves cytoplasm resides at potentially function as PgR. Since microbes employ conscription...
ABSTRACT Human plasminogen (hPg)-binding M-protein (PAM), a major virulence factor of Pattern D Streptococcus pyogenes (GAS), is the primary receptor responsible for binding and activating hPg. PAM covalently bound to cell wall (CW) through membrane (CM)-resident sortase A (SrtA)-catalyzed cleavage PAM-proximal C-terminal LPST ↓ -GEAA motif present immediately upstream its transmembrane domain (TMD), subsequent transpeptidation CW. These steps expose N-terminus extracellular milieu (EM)...
M-protein (PAM) largely contributes to the pathogenesis of Pattern D Group A Streptococcus pyogenes (GAS). However, mechanism complex formation is unknown. In a system consisting Class II PAM from GAS isolate NS88.2 (PAMNS88.2), with one K2hPg binding a-repeat in its A-domain, we employed biophysical techniques analyze K2hPg/PAMNS88.2 interaction. We show that apo-PAMNS88.2 coiled-coil homodimer (M.Wt. ~80 kDa) at 4°C - 25°C, and monomeric ~40 37°C, demonstrating temperature-dependent...