A5050345871- Antimicrobial Peptides and Activities
- Streptococcal Infections and Treatments
- Probiotics and Fermented Foods
- Biochemical and Structural Characterization
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Microbial Natural Products and Biosynthesis
- Pneumonia and Respiratory Infections
- Protein Hydrolysis and Bioactive Peptides
- Inflammasome and immune disorders
- Oral microbiology and periodontitis research
- Long-Term Effects of COVID-19
- RNA Research and Splicing
- Neonatal and Maternal Infections
- RNA and protein synthesis mechanisms
- Nanopore and Nanochannel Transport Studies
- COVID-19 Clinical Research Studies
University of Notre Dame
2018-2024
Houghton University
2023
Bacteriocins, the ribosomally produced antimicrobial peptides of bacteria, represent an untapped source promising antibiotic alternatives. However, bacteriocins display diverse mechanisms action, a narrow spectrum activity, and inherent challenges in natural product isolation making vitro verification putative difficult. A subset exert their effects through favorable biophysical interactions with bacterial membrane mediated by charge, hydrophobicity, conformation peptide. We have developed...
Group A Streptococcus (GAS, pyogenes ) is a Gram-positive human pathogen that employs several secreted and surface-bound virulence factors to manipulate its environment, allowing it cause variety of disease outcomes. One such factor Streptolysin S (SLS), ribosomally-produced peptide toxin undergoes extensive post-translational modifications. The activity SLS has been studied for over 100 years owing rapid potent ability lyse red blood cells, the shown play major role in GAS vivo . We have...
Invasive outcomes of Group A
The ribosomally produced, circularized bacteriocin enterocin AS-48 produced by Enterococcus sp. exhibits antibacterial activity through membrane disruption. penetrating of has been attributed to a specific alpha-helical region on the circular peptide. Truncated, linearized forms containing these domains have shown preserve limited bactericidal activity. We utilized amino acid sequence active helical domain perform homology-based search similar sequences in other bacterial genomes. identified...
A microfluidic platform is used to generate phase diagrams for formation of biomolecular condensates without conventional manual sample preparation methods.
The antimicrobial peptide (AMP) circularized bacteriocin enterocin AS-48 produced by
Abstract Bacteriocins are ribosomally produced antimicrobial peptides that represent an untapped source of promising antibiotic alternatives. However, inherent challenges in isolation and identification natural bacteriocins substantial yield have limited their potential use as viable compounds. In this study, we developed overall pipeline for bacteriocin-derived compound design testing combines sequence-free prediction using a machine-learning algorithm simple biophysical trait filter to...
Abstract Background Staphylococcus aureus ( S. ) is a major human pathogen owing to its arsenal of virulence factors, as well acquisition multi-antibiotic resistance. Here we report the identification Streptolysin S (SLS) like biosynthetic gene cluster in highly virulent community-acquired methicillin resistant (MRSA) isolate, JKD6159. Examination SLS-like JKD6159 shows significant homology and organization SLS-associated (sag) responsible for production hemolysin SLS Group A Streptococcus ....