A5048170064- Genomics and Phylogenetic Studies
- Streptococcal Infections and Treatments
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Antimicrobial Resistance in Staphylococcus
- interferon and immune responses
- Viral Infections and Immunology Research
- Neonatal and Maternal Infections
- Animal Disease Management and Epidemiology
- Bacterial Identification and Susceptibility Testing
- Pharmacogenetics and Drug Metabolism
- Vector-Borne Animal Diseases
- Cancer-related molecular mechanisms research
- Enzyme Production and Characterization
- Steroid Chemistry and Biochemistry
- Bacterial Genetics and Biotechnology
- Infective Endocarditis Diagnosis and Management
- Microbial Community Ecology and Physiology
- Genetic Associations and Epidemiology
- Polyamine Metabolism and Applications
- Axon Guidance and Neuronal Signaling
- RNA Research and Splicing
- Multiple Sclerosis Research Studies
- Rabies epidemiology and control
- Bacterial biofilms and quorum sensing
Hubei University
2020-2025
University of Notre Dame
2014-2025
Chinese Academy of Sciences
2014-2019
Tianjin Institute of Industrial Biotechnology
2014-2019
University of Hong Kong
2018
University of Essex
2017
Institute for Social and Economic Research
2017
Dongyang People's Hospital
2010
ABSTRACT Group A Streptococcus (GAS) is a major human pathogen that causes several invasive diseases including necrotizing fasciitis. The host coagulation cascade initiates fibrin clots to sequester bacteria prevent dissemination into deeper tissues. GAS, especially skin-tropic bacterial strains, utilize specific virulence factors, plasminogen binding M-protein (PAM) and streptokinase (SK), manipulate hemostasis activate cause fibrinolysis clot escape. unresolved question regards the...
Large-scale genome-wide association studies have reported EPHA1 rs11767557 variant to be associated with Alzheimer’s disease (AD) risk in the European population. However, it is still unclear how this functionally contributes underlying pathogenesis. The located approximately 3 kb upstream of gene. We think that may modify expression nearby genes such as and further cause AD risk. Until now, potential between has not been previous studies. Here, we evaluate using multiple large-scale eQTLs...
The steroidal hydroxylases CYP5150AP3 and CYP5150AN1 together with the previously characterized CYP5150AP2 belong to CYP5150A family of P450 enzymes high amino acid sequence identity, but they showed completely different regioselectivities toward 11-deoxycortisol, suggesting regioselectivity diversity CYP5150 family. They are also distinct from known bacterial fungal in substrate specificity regioselectivity. Biocatalytic hydroxylation is one important transformations for functionalization...
Genomic recombination plays an important role in driving adaptive evolution and population differentiation bacteria. However, controversy exists as to the effects of on diversity differentiation, i.e., is frequent enough sweep through at selected gene loci (gene-specific sweeps), or rate low without interfering genome-wide selective sweeps. Observations supporting either view are sparse. Pathogenic bacteria causing infectious diseases promising candidates provide observations recombination....
Abstract A novel marine strain representing efficient degradation ability toward brown algae was isolated, identified, and assigned to Bacillus weihaiensis Alg07. The alga-associated bacteria promote the nutrient cycle perform important functions in ecosystem. de novo sequencing of B. Alg07 genome carried out. Results gene annotation carbohydrate-active enzyme analysis showed that harbored enzymes can completely degrade alginate laminarin, which are specific polysaccharides algae. We also...
In this study, we identified a P450 enzyme (STH10) and an oxidoreductase (POR) from Thanatephorus cucumeris NBRC 6298 by combination of transcriptome sequencing heterologous expression in Pichia pastoris The biotransformation 11-deoxycortisol was performed using whole cells coexpressing sth10 por, the product analysis indicated that STH10 possessed steroidal 19- 11β-hydroxylase activities. This is novel fungal with 19-hydroxylase activity, which different known aromatase cytochrome 19...
Avian influenza A H5N1 virus can cause lethal disease in humans. The trigger severe pneumonia and lead to acute respiratory distress syndrome. Data from clinical, vitro vivo suggest that virus-induced cytokine dysregulation could be a contributory factor the pathogenesis of human disease. However, precise mechanism infection eliciting unique host response are still not well understood. To obtain better understanding molecular events at earliest time points, we used RNA-Seq quantify compare...
Classical swine fever (CSF) is a highly contagious disease of caused by classical virus (CSFV). For decades the has been controlled in China modified live vaccine (C-strain) genotype 1. The emergent 2 strains have become predominant past years that are genetically distant from strain. Here, we aimed to evaluate current infectious status CSF, and for this purpose 24 isolates CSFV were identified different areas during 2016–2018. Phylogenetic analysis NS5B, E2 full genome revealed new...
The first genome sequence of a group A Streptococcus pyogenes serotype M23 (emm23) strain (M23ND), isolated from an invasive human infection, has been completed. this opacity factor-negative (SOF(-)) is composed circular chromosome 1,846,477 bp. Gene profiling showed that contained six phage-encoded and 24 chromosomally inherited well-known virulence factors, as well 11 pseudogenes. bacterium acquired four large prophage elements, ΦM23ND.1 to ΦM23ND.4, harboring genes encoding streptococcal...
The genome of an invasive skin-tropic strain (AP53) serotype M53 group A Streptococcus pyogenes (GAS) is composed a circular chromosome 1,860,554 bp and carries genetic markers for infection at skin locales, viz, emm gene family pattern D FCT type 3. Through genome-scale comparisons AP53 with other GAS genomes, we identified 596 candidate single-nucleotide polymorphisms (SNPs) that reveal potential basis tropism. differed by ∼30 point mutations from noninvasive (Alab49), 4 which are located...
African swine fever virus (ASFV) is a lethal disease agent that causes high mortality in population and devastating loss industries. The development of efficacious vaccines has been hindered by the gap knowledge concerning genetic variation ASFV factors involved host adaptation virus-host interactions. In this study, we performed meta-genetic study aiming to profile landscape identify with signatures positive selection relevance adaptation. Our data reveal level variability shaped both...
The two-component control of virulence (Cov) regulator (R)-sensor (S) (CovRS) regulates the Streptococcus pyogenes (group A [GAS]). Inactivation CovS during infection switches pathogenicity GAS to a more invasive form by regulating transcription diverse genes via CovR. However, manner in which CovRS controls through expression extended gene families has not been fully determined. In current study, CovS-regulated profiles hypervirulent emm23 strain (M23ND/CovS negative [M23ND/CovS(-)]) and...
Phenotypes related to well-being (life satisfaction, positive affect, neuroticism, and depressive symptoms), are genetically highly correlated (| r g | > .75). Multivariate genome-wide analyses ( N obs = 958,149) of these traits, collectively referred as the spectrum, reveals 63 significant independent signals, which 29 were not previously identified. Transcriptome epigenome implicate variation in gene expression at 8 additional loci CpG methylation 6 etiology well-being. We leverage an...
Symmetric genomic rearrangements around replication axes in genomes are commonly observed prokaryotic genomes, including Group A Streptococcus (GAS). However, asymmetric rare. Our previous studies showed that the hypervirulent invasive GAS strain, M23ND, containing an inactivated transcriptional regulator system, covRS, exhibits unique extensive rearrangements, which reconstructed a structure distinct from other genomes. In current investigation, we identified rearrangement events and...
The M protein, a major virulence factor of Group A Streptococcus (GAS), is regulated by the multigene regulator Mga. An unexplained phenomena frequently occurring with in vitro genetic manipulation or culturing M1T1 GAS strains loss protein production. This study was aimed at elucidating basis for majority protein-negative (M-) variants had one C deletion tract 8 cytidines starting base 1,571 M1 mga gene, which designated as c.1571C[8]. led to c.1571C[7] variant that has an open reading...
Abstract African swine fever virus (ASFV) is a lethal disease agent that causes high mortality in population and devastating loss industries. The development of efficacious vaccines has been hindered by the gap knowledge concerning genetic variation ASFV factors involved host adaptation virus-host interactions. In this study, we performed meta-genetic study aiming to profile landscape identify with signatures positive selection relevance adaptation. Our data reveals level variability shaped...
<title>Abstract</title> Background Gene inactivations caused by loss-of-function mutations and regulatory changes insertions/deletions (InDels) are common genetic factors closely related to phenotypic diversity or pathogenic versatility of many bacterial species. However, these were usually ignored the computational approach genome-wide association studies (GWAS). It prevents full understanding contributions variants roles in shaping architecture species diverse phenotypes. Group A...