A5102888333- Natural product bioactivities and synthesis
- Chromatography in Natural Products
- Analytical Chemistry and Chromatography
- Plant biochemistry and biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Phytochemicals and Antioxidant Activities
- Toxin Mechanisms and Immunotoxins
- Pharmacological Effects of Natural Compounds
- Molecular spectroscopy and chirality
- Sirtuins and Resveratrol in Medicine
- Toxoplasma gondii Research Studies
- Phytoestrogen effects and research
- Phytochemistry and Biological Activities
- Plant Pathogenic Bacteria Studies
- Trypanosoma species research and implications
- Food composition and properties
- Microbial Natural Products and Biosynthesis
- Plant-Microbe Interactions and Immunity
- Insect and Arachnid Ecology and Behavior
- Legume Nitrogen Fixing Symbiosis
- Microbial Metabolites in Food Biotechnology
- Research on Leishmaniasis Studies
- Insect Utilization and Effects
- Olfactory and Sensory Function Studies
- Soybean genetics and cultivation
Centre National de la Recherche Scientifique
2012-2021
Université de Reims Champagne-Ardenne
2012-2021
Institut de Chimie
2021
Institut de Chimie Moléculaire de Reims
2012-2020
Université de Toulouse
2012-2018
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
2010-2018
Institut de Chimie des Substances Naturelles
2013-2018
Institut National des Sciences Appliquées de Toulouse
2018
Université Toulouse III - Paul Sabatier
2012-2013
Institut National de la Recherche Agronomique
2012
Abstract Plant resistance to phytopathogenic microorganisms mainly relies on the activation of an innate immune response usually launched after recognition by plant cells microbe-associated molecular patterns. The hormones, salicylic acid (SA), jasmonic acid, and ethylene have emerged as key players in signaling networks involved immunity. Rhamnolipids (RLs) are glycolipids produced bacteria surface motility biofilm development. Here we report that RLs trigger Arabidopsis (Arabidopsis...
Because of their highly complex metabolite profile, the chemical characterization bioactive natural extracts usually requires time-consuming multistep purification procedures to achieve structural elucidation pure individual metabolites. The aim present work was develop a dereplication strategy for identification metabolites directly within mixtures. Exploiting polarity range metabolites, principle rapidly fractionate multigram quantity crude extract by centrifugal partition extraction...
Toxoplasma gondii is a cosmopolitan protozoan parasite which affects approximately 30% of the population worldwide. The drugs currently used against toxoplasmosis are few in number and show several limitations, such as drug intolerance, poor bioavailability, or resistance mechanism developed by parasite. Thus, it important to find new compounds able inhibit invasion proliferation. In this study, 400 open-access Pathogen Box, provided Medicines for Malaria Venture (MMV) foundation, were...
In the present study, resveratrol and various oligomeric derivatives were obtained from a 14 L bioreactor culture of elicited grapevine cell suspensions (Vitis labrusca L.). The crude ethyl acetate stilbene extract medium was fractionated by centrifugal partition chromatography (CPC) using gradient elution method major stilbenes contained in fractions subsequently identified 13C-NMR-based dereplication procedure further 2D NMR analyses including HSQC, HMBC, COSY. Beside δ-viniferin (2),...
All living organisms depend on NADPH production to feed essential biosyntheses and for oxidative stress defense. Protozoan parasites such as the sleeping sickness pathogen Trypanosoma brucei adapt different host environments, carbon sources, stresses during their infectious life cycle. The procyclic stage develops in midgut of tsetse insect vector, where they rely proline source, although prefer glucose when grown rich media. Here, we investigate flexible source-dependent use synthesis...
Plant innate immunity is activated upon perception of invasion pattern molecules by plant cell-surface immune receptors. Several bacteria the genera Pseudomonas and Burkholderia produce rhamnolipids (RLs) from l-rhamnose (R)-3-hydroxyalkanoate precursors (HAAs). RL HAA secretion required to modulate bacterial surface motility, biofilm development, thus successful colonization hosts. Here, we show that lipidic secretome opportunistic pathogen aeruginosa, mainly comprising RLs HAAs, stimulates...
Coffee silverskin (CS) is the thin epidermis covering and protecting coffee bean it represents main by-product of roasting process. CS has recently gained attention due to its high content in bioactive molecules growing interest valuable reutilization waste products. Drawing inspiration from biological function, here potential cosmetic applications was investigated. recovered one largest roasters located Switzerland processed through supercritical CO2 extraction, thereby generating extract....
A computer-aided, 13C NMR-based dereplication method is presented for the chemical profiling of natural extracts without any fractionation. An algorithm was developed in order to compare NMR shifts obtained from a single routine spectrum with set predicted data stored metabolite database. The evaluates quality matching between experimental and by calculating score function returns list metabolites that are most likely be present studied extract. proof principle demonstrated on crude alkaloid...
Wood residues produced from forestry activities represent an interesting source of biologically active, high value-added secondary metabolites. In this study, 30 extracts 10 barks deciduous and coniferous tree species were investigated for their potential dermo-cosmetic use. The obtained <i>Fagus sylvatica, Quercus robur, Alnus glutinosa, Prunus avium, Acer pseudoplatanus, Fraxinus excelsior, Populus robusta, Larix decidua, Picea abies</i>, <i>Populus tremula</i> after three successive...
Common spruce (Picea abies L.) is a fast-growing coniferous tree, widely used in several countries for the production of sawn wood, timber and pulp. During this industrial exploitation, large quantities barks are generated as waste materials. The aim study was bio-guided investigation effective recovery methanol-soluble metabolites common bark development new dermo-cosmetic agents. active methanol extract initially fractionated by Centrifugal Partition Chromatography (CPC) using triphasic...
In the glucose-free environment that is midgut of tsetse fly vector, procyclic form Trypanosoma brucei primarily uses proline to feed its central carbon and energy metabolism. these conditions, parasite needs produce glucose 6-phosphate (G6P) through gluconeogenesis from metabolism non-glycolytic source(s). We showed here two phosphoenolpyruvate-producing enzymes, PEP carboxykinase (PEPCK) pyruvate phosphate dikinase (PPDK) have a redundant function for essential proline. Indeed,...
Soyasaponins are phytochemicals of major interest for health. Their identification and quantification remain difficult owing to the large number structural isomers in soybeans lack stable standards. In this study, a rapid method using high performance liquid chromatography (HPLC) UV detector (205 nm) was developed identify quantify soyasaponins belonging group B compare them with isoflavones different soy materials. 2,3-Dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP)-conjugated were...
Although resveratrol (3,5,4′-trihydroxy-stilbene) is one of the most studied natural product because its biological properties such as antioxidant or anticancer, low water solubility and stability (influenced by pH, light increased temperature), well propensity to oligomerize, limit bioavailability applications in nutraceutic, cosmetics pharmaceutical industries. Besides, production at multigram scale through vine cell culture limited tedious extraction pure from bioconversion medium due...
Trypanosoma brucei is a parasitic protist that undergoes complex life cycle during transmission from its mammalian host (bloodstream forms) to the midgut of insect vector (procyclic form). In both forms, most glycolytic steps take place within specialized peroxisomes, called glycosomes. Here, we studied metabolic adaptations in procyclic trypanosome mutants affected their maintenance glycosomal redox balance. T. can theoretically use three strategies maintain NAD(+)/NADH balance as follows:...
Resveratrol and related oligostilbenes are defense molecules produced by grapevine in response to stresses including various elicitors or signal molecules. Together with their prominent role planta, these compounds have been the center of much attention recent decades due pharmacological properties. The cost-effective production resveratrol derivatives such as viniferins more structurally complex stilbene oligomers remains a challenging task. In this study, chemical diversity stilbenes Vitis...
Abstract Introduction Alphitonia neocaledonica (Rhamnaceae) is an endemic tree of New Caledonia. Although three flavonoids have been identified in the leaves, secondary metabolite profile fruits has never investigated. Objective Phytochemical investigation A. and evaluation their anti‐oxidant, anti‐tyrosinase cytotoxic activities. Methods A hydromethanolic extract was fractionated by liquid–liquid extraction to obtain ethyl acetate n ‐butanolic fractions. The ethyl‐acetate‐soluble part...