A5046439509- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Plant Parasitism and Resistance
- Carbohydrate Chemistry and Synthesis
- Plant Pathogens and Fungal Diseases
- Transgenic Plants and Applications
- Plant biochemistry and biosynthesis
- Microbial bioremediation and biosurfactants
- Phytochemical compounds biological activities
- Plant tissue culture and regeneration
- African Botany and Ecology Studies
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Soybean genetics and cultivation
- Fern and Epiphyte Biology
- Fungal Plant Pathogen Control
- Plant Virus Research Studies
- Glycosylation and Glycoproteins Research
- Biofuel production and bioconversion
- Pesticide and Herbicide Environmental Studies
- Plant Stress Responses and Tolerance
- Potato Plant Research
- Genomics, phytochemicals, and oxidative stress
- Polysaccharides and Plant Cell Walls
Université de Reims Champagne-Ardenne
2015-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021-2024
Résistance Induite et Bioprotection des Plantes
2014-2024
Centre National de la Recherche Scientifique
2017-2023
CNR de la Résistance aux Antibiotiques
2014-2020
Comité Champagne
2015-2016
University of Liège
2013
Institut de Chimie Moléculaire de Reims
2012
Zero to Three
2004
Institut de Biologie Moléculaire des Plantes
1999-2002
A fatty acid triggers immune responses Plants and animals respond to the microbial communities around them, whether in antagonistic or mutualistic ways. Some of these interactions are mediated by lipopolysaccharide—a large, complex, irregular molecule on surface most Gram-negative bacteria. Studying small mustard plant Arabidopsis , Kutschera et al. identified a 3-hydroxydecanoyl chain as structural element sensed plant's lectin receptor kinase. Indeed, synthetic 3-hydroxydecanoic alone was...
ABSTRACT Rhamnolipids produced by the bacteria Pseudomonas aeruginosa are known as very efficient biosurfactant molecules. They used for a wide range of industrial applications, especially in food, cosmetics and pharmaceutical formulations well bioremediation pollutants. In this paper, role rhamnolipids novel molecules triggering defence responses protection against fungus Botrytis cinerea grapevine is presented. The effect was assessed using cell suspension cultures vitro‐plantlets. Ca 2+...
Summary The role of flagellin perception in the context plant beneficial bacteria still remains unclear. Here, we characterized sensing system flg22– FLAGELLIN SENSING 2 ( FLS 2) grapevine, and analyzed interaction with endophytic growth‐promoting rhizobacterium PGPR ) B urkholderia phytofirmans . functionality grapevine receptor, Vv 2, was demonstrated by complementation assays A rabidopsis thaliana fls2 mutant, which restored flg22‐induced H O production growth inhibition. Using synthetic...
Non-self-recognition of microorganisms partly relies on the perception microbe-associated molecular patterns (MAMPs) and leads to activation an innate immune response. Bacillus subtilis produces three main families cyclic lipopeptides (LPs), namely surfactins, iturins fengycins. Although LPs are involved in induced systemic resistance (ISR) activation, little is known about defence responses by these molecules their involvement local fungi. Here, we showed that purified surfactin,...
Plant growth-promoting rhizobacteria (PGPR) are beneficial microorganisms that colonize the rhizosphere of many plant species and confer effects, such as an increase in growth. PGPR also well known inducers systemic resistance to pathogens plants. However, molecular mechanisms involved locally after direct perception these bacteria by cells still remain largely unknown. Burkholderia phytofirmans strain PsJN is endophytic colonizes grapevine protects against grey mould disease caused Botrytis...
Abstract Plant resistance to phytopathogenic microorganisms mainly relies on the activation of an innate immune response usually launched after recognition by plant cells microbe-associated molecular patterns. The hormones, salicylic acid (SA), jasmonic acid, and ethylene have emerged as key players in signaling networks involved immunity. Rhamnolipids (RLs) are glycolipids produced bacteria surface motility biofilm development. Here we report that RLs trigger Arabidopsis (Arabidopsis...
We have analyzed the spatial and temporal expression of defense responses induced in attached tobacco leaves treated with a Phytophthora megasperma glycoprotein that was previously shown to be an efficient elicitor hypersensitive reaction. The infiltrated tissue (zone 1), surrounding 2), which is 5 mm width, at distance >2 cm from zone 2 3) were separately. Cell death occurred only 1 completed by 14 h. Defense gene zones striking differential patterns, but not 3. There rapid (1 4 h)...
Summary The Arabidopsis RPS4 gene belongs to the Toll/interleukin‐1 receptor/nucleotide‐binding site/leucine‐rich repeat (TIR‐NB‐LRR) class of plant resistance ( R ) genes. It confers Pseudomonas syringae carrying avirulence avrRps4 . Transient expression genomic driven by 35S promoter in tobacco leaves induces an AvrRps4‐independent hypersensitive response (HR). same phenotype is seen after a full‐length cDNA. This indicates that alternative splicing not involved this HR. extent HR...
Abstract H2O2 from the oxidative burst, cell death, and defense responses such as production of phenylalanine ammonia lyase (PAL), salicylic acid (SA), scopoletin were analyzed in cultured tobacco (Nicotiana tabacum) cells treated with three proteinaceous elicitors: two elicitins (α-megaspermin β-megaspermin) one glycoprotein. These proteins have been isolated Phytophthora megasperma H20 previously shown to be equally efficient inducing a hypersensitive response (HR) upon infiltration into...
Some plant-associated bacteria such as Bacillus sp. can protect their host from pathogen ingress and this biocontrol activity correlates with potential to form multiple antibiotics upon in vitro growth. However, our knowledge on antibiotic production by soil bacilli evolving roots natural conditions is still limited. In work, antibiome imaging first revealed that the lipopeptide surfactin main bacterial ingredient produced planta within hours of interaction root tissues. We further...
Rhamnolipids (RLs) are amphiphilic molecules naturally produced by some bacteria with a large range of biological activities. Although studies report their potential interest in plant protection, evaluation effects and efficiency on annual crops worldwide agronomic is lacking. The main objective this work was to investigate elicitor protective activities rapeseed crop species while evaluating physiological effects. Here we that RLs from Pseudomonas aeruginosa secretome trigger an effective...
Sphingolipids are emerging as second messengers in programmed cell death and plant defense mechanisms. However, their role is far from being understood, especially against necrotrophic pathogens. Sphingolipidomics responses during pathogenic infection were evaluated the mutant of long-chain base phosphate (LCB-P) lyase, encoded by dihydrosphingosine-1-phosphate lyase1 (AtDPL1) gene regulating base/LCB-P homeostasis. Atdpl1 mutants exhibit tolerance to fungus Botrytis cinerea but...
Bacteria from the Pantoea genus are regularly found to interact with a variety of plants, as pathogens or beneficial bacteria, depending on strain and host plant. Despite increasing interest in this bacterial genus, mechanisms by which it interacts plants remain be deciphered. This review addresses current knowledge about various types interactions metabolic traits spp. protecting against pathogens, direct antagonism stimulation plant innate immunity. The biocontrol capacity species mainly...
Expression of tobacco class I (CAT1) and II (CAT2) catalases was analyzed in leaves reacting hypersensitively to mosaic virus (TMV) or a fungal glycoprotein elicitor. In TMV-infected plants, Cat1 transcript levels declined rapidly while Cat2 transcripts accumulated strongly. The spatial temporal changes catalase transcripts, proteins, activity during the hypersensitive reaction (HR) were further investigated infiltrated with Two functionally different zones discriminated: tissue which cells...
Plant innate immunity is activated upon perception of invasion pattern molecules by plant cell-surface immune receptors. Several bacteria the genera Pseudomonas and Burkholderia produce rhamnolipids (RLs) from l-rhamnose (R)-3-hydroxyalkanoate precursors (HAAs). RL HAA secretion required to modulate bacterial surface motility, biofilm development, thus successful colonization hosts. Here, we show that lipidic secretome opportunistic pathogen aeruginosa, mainly comprising RLs HAAs, stimulates...
Natural rhamnolipids are potential biocontrol agents for plant protection against bacterial and fungal diseases. In this work, we synthetized new synthetic mono-rhamnolipids (smRLs) consisting in a rhamnose connected to simple acyl chain differing by the nature of link length lipid tail. We then investigated effects these ether, ester, carbamate or succinate smRL derivatives on Botrytis cinerea development, symptoms spreading tomato leaves immune responses plants. Our results demonstrate...
Abstract Stimulation of plant innate immunity by natural and synthetic elicitors is a promising alternative to conventional pesticides for more sustainable agriculture. Sugar-based bolaamphiphiles are known their biocompatibility, biodegradability low toxicity. In this work, we show that Synthetic Rhamnolipid Bolaforms (SRBs) have been synthesized green chemistry trigger Arabidopsis immunity. Using structure-function analysis, demonstrate SRBs, depending on the acyl chain length,...