A5079996044- Trypanosoma species research and implications
- Biochemical and Molecular Research
- Research on Leishmaniasis Studies
- Antifungal resistance and susceptibility
- Lysosomal Storage Disorders Research
- Fungal and yeast genetics research
- Plant-Microbe Interactions and Immunity
- Microbial Metabolites in Food Biotechnology
- ATP Synthase and ATPases Research
- Calcium signaling and nucleotide metabolism
- Insect-Plant Interactions and Control
- Fungal Infections and Studies
Laboratoire de Microbiologie Fondamentale et Pathogénicité
2021
Centre National de la Recherche Scientifique
2012-2020
Université de Bordeaux
2013-2020
Institut Polytechnique de Bordeaux
2018-2020
Centre de Résonance Magnétique des Systèmes Biologiques
2012-2018
Université de Tours
2010-2011
Metabolomics coupled with heavy-atom isotope-labelled glucose has been used to probe the metabolic pathways active in cultured bloodstream form trypomastigotes of Trypanosoma brucei, a parasite responsible for human African trypanosomiasis. Glucose enters many branches metabolism beyond glycolysis, which widely held be sole route metabolism. Whilst pyruvate is major end-product catabolism, its transamination product, alanine, also produced significant quantities. The oxidative branch pentose...
Background The bloodstream forms of Trypanosoma brucei, the causative agent sleeping sickness, rely solely on glycolysis for ATP production. It is generally accepted that pyruvate major end-product excreted from glucose metabolism by proliferative long-slender parasite, with virtually no production succinate and acetate, main end-products all other trypanosomatid adaptative forms, including procyclic insect form T. brucei. Methodology/Principal Findings A comparative NMR analysis showed...
The Trypanosoma brucei procyclic form resides within the digestive tract of its insect vector, where it exploits amino acids as carbon sources. Threonine is acid most rapidly consumed by this parasite, however role poorly understood. Here, we show that trypanosomes grown in rich medium only use glucose and threonine for lipid biosynthesis, with threonine's contribution being ∼ 2.5 times higher than glucose. A combination reverse genetics NMR analysis excreted end-products from metabolism,...
In the glucose-free environment that is midgut of tsetse fly vector, procyclic form Trypanosoma brucei primarily uses proline to feed its central carbon and energy metabolism. these conditions, parasite needs produce glucose 6-phosphate (G6P) through gluconeogenesis from metabolism non-glycolytic source(s). We showed here two phosphoenolpyruvate-producing enzymes, PEP carboxykinase (PEPCK) pyruvate phosphate dikinase (PPDK) have a redundant function for essential proline. Indeed,...
Insect stage trypanosomes use an "acetate shuttle" to transfer mitochondrial acetyl-CoA the cytosol for essential fatty acid biosynthesis. The acetate sources are acetate:succinate CoA-transferase (ASCT) and unknown enzymatic activity. We have identified a gene encoding thioesterase (ACH) activity, which is shown be second source. First, RNAi-mediated repression of ASCT in ACH null background abolishes production from glucose, as opposed both single mutants. Second, incorporation...
De novo biosynthesis of lipids is essential for Trypanosoma brucei, a protist responsible the sleeping sickness. Here, we demonstrate that ketogenic carbon sources, threonine, acetate and glucose, are precursors both fatty acid sterol synthesis, while leucine only contributes to production in tsetse fly midgut stage parasite. Degradation these sources into was investigated using combination reverse genetics analysis radio-labelled incorporation lipids. For instance, (i) deletion gene...
Candida guilliermondii is an opportunistic emerging fungal agent of candidiasis often associated with oncology patients. This yeast also remains interesting biotechnological model for the industrial production value-added metabolites. The recent whole-genome sequencing C. ATCC 6260 reference strain provides resource elucidating new molecular events supporting pathogenicity, antifungal resistance and exploring potential metabolic engineering. In present study, we designed efficient...