A5063303446- Advanced Fluorescence Microscopy Techniques
- Breast Cancer Treatment Studies
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Thyroid Cancer Diagnosis and Treatment
- Cervical Cancer and HPV Research
- Breast Lesions and Carcinomas
- Ovarian cancer diagnosis and treatment
- Biomarkers in Disease Mechanisms
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Lymphoma Diagnosis and Treatment
- Photodynamic Therapy Research Studies
- Photoacoustic and Ultrasonic Imaging
- Sarcoma Diagnosis and Treatment
- Vascular Tumors and Angiosarcomas
- Radiology practices and education
- Renal and related cancers
- Bioinformatics and Genomic Networks
- PARP inhibition in cancer therapy
- HER2/EGFR in Cancer Research
- Kruppel-like factors research
- Colorectal Cancer Screening and Detection
- melanin and skin pigmentation
- Neurofibromatosis and Schwannoma Cases
D’Or Institute for Research and Education
2022-2024
Hospital São Paulo
2023
Universidade Estadual de Campinas (UNICAMP)
2014-2022
Hospital de Clínicas da Unicamp
2016
Second-harmonic generation microscopy represents an important tool to evaluate extracellular matrix collagen structure, which undergoes changes during cancer progression. Thus, it is potentially relevant assess breast development. We propose the use of second-harmonic images tumor stroma selected on hematoxylin and eosin–stained slides prognostic value fibers analyses in peri intratumoral areas patients diagnosed with invasive ductal carcinoma. Quantitative parameters were performed using...
To evaluate the diagnostic and prognostic value of immunohistochemical expression WT1, p53 p16 in low- (LGSOCs) high-grade serous ovarian carcinomas (HGSOCs).HGSOC had a significantly higher proportion advanced stage disease, CA125 levels, post-surgery residual disease recurrence or progression. WT1 was expressed 71.4% LGSOCs 57.1% HGSOCs (p = 0.32). Focal and/or complete absence with negative found 90.5% LGSOCs, contrast to 88.1% diffuse positive (<0.001). The IHC p53/p16 index...
Abstract One of the promising tools to evaluate collagen in extracellular matrix is second-harmonic generation microscopy (SHG). This approach may shed light on biological behavior cancers and their taxonomy, but has not yet been applied characterize fibers cases diagnosed as invasive breast carcinoma (BC) histological special types (IBC-ST). Tissue sections from 99 patients with IBC-ST 21 no type (IBC-NST) were submitted evaluation parameters by SHG. microarray was performed...
The objective of this study is to evaluate the relationship between discoidin domain receptor 2 (DDR2) and miR-182 expression with response platinum-based chemotherapy survival in women high-grade serous ovarian cancer (HGSOC). We evaluated 78 HGSOC stages I-IV, diagnosed 1996 2013, followed up until 2016. DDR2 was assessed using immunohistochemistry on tissue microarray slides. microRNAs were by qRT-PCR. high 11 (14.1%) women. PFS significantly lower FIGO stage I/II – versus III/IV,...
Abstract Extracellular matrix (ECM) represents more than a mere intercellular cement. It is physiologically active in cell communication, adhesion and proliferation. Collagen the most abundant protein, making up to 90% of ECM, 30% total protein weight humans. Second-harmonic generation (SHG) microscopy an important tool study collagen organization ECM freshly unfixed tissues paraffin-embedded tissue samples. This manuscript aims review some applications SHG Oncologic Pathology, mainly...
Abstract Thyroid cancer is one of the most common types worldwide. It a spectrum different diseases, ranging from very indolent to lethal tumors. Differentiated Carcinoma (DTC), thyroid malignancy, has often an excellent prognosis, but some patients develop metastatic Radioiodine-Refractory disease (RAIR) that cannot be controlled locally. In this setting, and for with Medullary (MTC) Anaplastic (ATC), systemic treatment non-selective Multikinase Inhibitors (MKIs) employed improve survival...
<h3>Objective</h3> The purpose of this study was to compare the immunohistochemical expression BRCA1, Ki67, and β-catenin in women with low-grade (LGSOC) high-grade serous ovarian carcinomas (HGSOC) their relationship clinicopathological features, response platinum-based chemotherapy, survival. <h3>Methods</h3> For study, 21 LGSOC 85 HGSOC stage I IV cases, diagnosed treated from 1996 2013 followed-up until December 2016, were included. assessed using tissue microarray-based...
Introduction: In breast cancer (BC), desmoplastic reaction, assembled primarily by fibroblasts, is associated with unfavorable prognosis, but the reason of this fact remains still unclear. context, nonlinear optics microscopy, including Fluorescence Lifetime Imaging Microscopy (FLIM), has provided advancement in cellular metabolism research. paper, our purpose to differentiate BC cells or without contact reaction. Formalin fixed, paraffin embedded samples were used at different points...
Abstract The extracellular matrix plays an important role in cellular balance, and collagen fibers are its most component. Over the last few years, second harmonic generation (SHG) microscopy has been used for analysis of several types gynaecological cancers, such as breast ovarian cancer. value parameters obtained with this technique to gain insights on physiopathology prognostic evaluation cancer advocated. Herein, we have characterized squamous cell carcinoma (VSCC) preneoplastic lesions...
Introduction: In breast cancer (BC), desmoplastic reaction, assembled primarily by fibroblasts, is associated with unfavorable prognosis, but the reason of this fact remains still unclear. context, nonlinear optics microscopy, including Fluorescence Lifetime Imaging Microscopy (FLIM), has provided advancement in cellular metabolism research. paper, our purpose to differentiate BC cells or without contact reaction. Formalin fixed, paraffin embedded samples were used at different points...
Breast cancer outcomes among patients who use safety-net hospitals in the highly populated Harris County, Texas and Southeast Brazil are poor. It is unknown whether treatment delay contributes to these outcomes.We conducted a retrospective cohort analysis of with non-metastatic breast diagnosed between January 1, 2009 December 31, 2011 at Health Unicamp's Women's Hospital, Barretos Brazilian National Institute Cancer, Brazil. We used Cox proportional hazards regression evaluate association...
In melanoma, collagen has been described as a potential new prognostic biomarker.In this way, our purpose was to evaluate the quantity, uniformity and organization of peri-and intratumoral collagen, correlate these parameters with clinicopathological features.For purpose, total 22 specimens cutaneous melanoma were submitted second-harmonic generation microscopy image evaluation.Our results suggest that fiber parameters, mainly organization, are candidates determine higher precision border...
Introduction Overall survival of women with high‐grade serous ovarian cancer (HGSOC) is highly related platinum resistance. Parameters collagen can be used as potential new prognostic biomarkers, since their relation tumor growth, invasion and metastasis has been demonstrated. These parameters are (1) quantity which the amount fibers deposited, (2) uniformity refers to how closely deposited side by (3) organization arranged. Aim To evaluate association intratumoral fiber resistance in HGSOC...
Abstract Background The coronavirus disease 2019 pandemic prompted changes in medical practice, with a reduction cytopathology volumes and relative increase the malignancy rate during lockdown initial postlockdown period. To date, no study has evaluated impact of these on volume rapid on‐site evaluation (ROSE) or frequency cases according to Bethesda System for Reporting Thyroid Cytopathology (TBSRTC) categories after vaccination. Methods Ultrasound‐guided thyroid fine‐needle aspiration...
Abstract Introduction: High-grade serous ovarian carcinoma (HGSOC) is a heterogeneous disease with high mortality. Initially most women respond to platinum chemotherapy, but rapidly many become resistant the drug and progress relapse death. Better knowledge of pathways responsible for mechanisms invasion metastasis in HGSOC may help identification prognostic biomarkers development new target therapies. The epithelial-mesenchymal transition (EMT) an important cellular process related...