A5049338658- Nicotinic Acetylcholine Receptors Study
- Autoimmune Bullous Skin Diseases
- Urticaria and Related Conditions
- Osteoarthritis Treatment and Mechanisms
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Receptor Mechanisms and Signaling
- Ion channel regulation and function
- Inflammatory mediators and NSAID effects
- Immune Response and Inflammation
- Insect and Pesticide Research
- Hair Growth and Disorders
- Cell Adhesion Molecules Research
- Knee injuries and reconstruction techniques
- Synthesis and Biological Evaluation
- COVID-19 Clinical Research Studies
- Chemokine receptors and signaling
- Monoclonal and Polyclonal Antibodies Research
- Skin and Cellular Biology Research
- Immune Cell Function and Interaction
- Blood disorders and treatments
- Neuroblastoma Research and Treatments
- Retinoids in leukemia and cellular processes
- Immunotherapy and Immune Responses
- Toxin Mechanisms and Immunotoxins
- Cholinesterase and Neurodegenerative Diseases
Institut Pasteur in Ho Chi Minh City
2023
University of Pennsylvania
2015-2022
Roseman University of Health Sciences
2019
Hershey (United States)
2015
University of Pittsburgh
2015
American Society of Plastic Surgeons
2015
Children's Hospital of Philadelphia
2007-2013
Rainbow Babies & Children's Hospital
2013
University of California, Davis
1998-2004
University of California Davis Medical Center
2000-2001
Several ganglionic nicotinic acetylcholine receptor (nAChR) types are abundantly expressed in nonneuronal locations, but their functions remain unknown. We found that keratinocyte α7 nAChR controls homeostasis and terminal differentiation of epidermal keratinocytes required for formation the skin barrier. The effects functional inactivation on cell cycle progression, differentiation, apoptosis were studied monolayers treated with α-bungarotoxin or antisense oligonucleotides Acra7 homozygous...
Because pemphigus vulgaris (PV) IgGs adsorbed on the rDsg3-Ig-His baculoprotein induced blisters in neonatal mice, it was proposed that anti-desmoglein 3 (Dsg 3) autoantibody causes PV. However, we found absorbs autoantibodies to different antigens, including a non-Dsg keratinocyte protein of 130 kDa. This prompted our search for novel targets PV autoimmunity. The IgG eluted from 75-kDa band both stained epidermis pemphigus-like pattern and acantholysis monolayers. Screening λgt11 cDNA...
A non-neuronal cholinergic system that includes neuronal-like nicotinic acetylcholine receptors (nAChRs) has recently been described in epithelial cells line the skin and upper respiratory tract. Since use of nicotine-containing products is associated with morbidity digestive tract, since nicotine may alter cellular functions directly via nAChRs, we sought to identify characterize a gingival esophageal epithelia. mRNA transcripts for alpha3, alpha5, alpha7, beta2 nAChR subunits, choline...
To determine whether nondesmoglein (non-Dsg) autoantibodies are pathogenic and they recognize keratinocyte cholinergic receptors that control cell adhesion because antikeratinocyte autoimmunity in patients with pemphigus vulgaris is not limited to the development of Dsg.To non-DSg pathogenic, we sought induce genetically engineered neonatal mice lacking Dsg 3 using IgGs did cross-react 1. involves receptors, latter were separated from membranes human keratinocytes, tagged covalent label...
Smoking is associated with aberrant cutaneous tissue remodeling, such as precocious skin aging and impaired wound healing. The mechanism not fully understood. Dermal fibroblasts (DF) are the primary cellular component of dermis may provide a target for pathobiologic effects tobacco products. purpose this study was to characterize nicotine (Nic) on growth remodeling function DF. We hypothesized that Nic DF result from its binding specific nicotinic acetylcholine receptors (nAChRs) expressed...
Pemphigus vulgaris (PV) is a life-threatening autoimmune disease of skin adhesion associated with IgG autoantibodies against keratinocytes (KC). Treatment PV systemic corticosteroids life-saving, but the mechanism therapeutic action has not been fully understood. We have developed an animal model that demonstrates methylprednisolone (MP) can block IgG-induced acantholysis, decreasing extent keratinocyte detachment in epidermis 3-5-day-old nude mice from 77.5 +/- 0.6 to 24.1 1.5% (p < 0.05)....
Abstract Background Poly(ADP‐ribose) polymerase (PARP) signals DNA damage and facilitates repair. PARP inhibitors are being evaluated in cancers with defective repair mechanisms or combination cytotoxic therapy radiation. We the inhibitor, olaparib, chemotherapy using vitro vivo pediatric solid tumor models. Procedure The IC 50 of olaparib alone agents was determined 10 cell lines. Synergy assessed index Chou‐Talalay. Olaparib topotecan/cyclophosphamide xenograft models Ewing sarcoma (RD‐ES)...
<h3>Background</h3> Pemphigus vulgaris (PV) is an autoimmune, IgG autoantibody–mediated disease of skin and mucosa leading to progressive blistering nonhealingerosions. Patients develop autoantibodies adhesion molecules mediating intercellular keratinocyte cholinergic receptors regulatingcell adhesion. <h3>Observations</h3> To determine whether a agonist can abolish PV IgG–induced acantholysis, litter mates neonatal athymic nude mice were injected withPV together with carbachol (0.04 µg/g...
The programmed cell death of the stratified squamous epithelial cells comprising human epidermis culminates in abrupt transition viable granular keratinocytes (KC) into dead corneocytes sloughed by skin. cell-corneocyte is associated with a loss volume and dry weight but mechanism for biological significance this form keratinocyte apoptosis remain obscure. We show that terminally differentiated KC extrude intercellular spaces living cytoplasmic buds containing randomly congregated components...
Objective CD14 is a monocyte/macrophage pattern-recognition receptor that modulates innate inflammatory signaling. Soluble levels in knee OA synovial fluids are associated with symptoms and progression of disease. Here we investigate the role this development using murine joint injury model Methods 10-week-old Male C57BL/6 (WT) CD14-deficient (CD14-/-) mice underwent destabilization medial meniscus (DMM) surgery to induce OA. Joint histopathology was used examine cartilage damage, microCT...
Self or foreign cellular proteins provide peptides for presentation by major histocompatibility complex (MHC) class I molecules on the surface of antigen presenting cells (APC). Surprisingly, several studies have shown that T-cells can recognize APC transfected with genes were not present in appropriate translational context. To understand basis this phenomenon, DNA constructs encoding OVA257-264 (SL8) peptide, but varying translation initiation codons. We report that, addition to ATG, 6...