A5053724669- Reproductive Biology and Fertility
- Renal and related cancers
- Pluripotent Stem Cells Research
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- Prenatal Screening and Diagnostics
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Gene expression and cancer classification
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive Health and Technologies
- Ovarian function and disorders
- Endometriosis Research and Treatment
- Uterine Myomas and Treatments
- Omental and Epiploic Conditions
- Gynecological conditions and treatments
- Neonatal Respiratory Health Research
- Lymphoma Diagnosis and Treatment
- Forensic and Genetic Research
- Ovarian cancer diagnosis and treatment
- Genomic variations and chromosomal abnormalities
- Appendicitis Diagnosis and Management
- Mercury impact and mitigation studies
- Hernia repair and management
- Reproductive System and Pregnancy
Akita University Hospital
2024
Akita University
2018-2024
Juntendo University Shizuoka Hospital
2024
Akita Red Cross Hospital
2022
Sendai Kousei Hospital
2020
Abstract During human embryonic development, early cleavage-stage embryos are more susceptible to errors. Studies have shown that many problems occur during the first mitosis, such as direct cleavage, chromosome segregation errors, and multinucleation. However, mechanisms whereby these errors mitosis in remain unknown. To clarify this aspect, present study, we image discarded living two-pronuclear stage zygotes using fluorescent labeling confocal microscopy without microinjection of DNA or...
This prospective study evaluated the accuracy of non-invasive preimplantation genetic testing for aneuploidy (niPGT-A) using cell-free DNA in spent culture medium, as well that (PGT-A) trophectoderm (TE) biopsy after culturing beyond implantation. Twenty frozen blastocysts donated by 12 patients who underwent IVF at our institution were investigated. Of these, 10 on day 5 and 6. Spent medium TE cells collected from each blastocyst thawing, embryos cultured vitro up to days. The outgrowths...
Recent studies reported morphokinetic indices for optimal selection of embryos in assisted reproductive technology (ART). The morphokinetics blastocyst stage include the collapse and re-expansion rates after thawing. However, evaluation methods using these have not been established, mainly because underlying molecular mechanisms remain unclarified. In this study, we focused on relationship between observation behaviour number cells constituting blastocyst. We evaluated 38 surplus human...
Uterine adenomyosis is a benign disease, common among women in their 40 and 50 s, characterised by ectopic endometrial tissue the uterine myometrial layer. Adenomyosis causes infertility has negative effect on outcomes of vitro fertilisation (IVF)/intracytoplasmic sperm injection (ICSI) embryo transfer (ET) cycles. It also been reported to have different characteristics depending adenomyotic lesion localisation. The its localisation IVF/ICSI-ET unclear. This study aimed investigate whether...
Studies have shown that some electrolytes, including Na + and K , play important roles in embryonic development. However, these studies evaluated electrolytes by using inhibitors or knockout mice, with no mention on the changes intracellular electrolyte concentrations during embryogenesis. In this study, we used indicators CoroNa Green AM ION Potassium Green-2 to directly visualise of respectively, at each developmental stage mouse embryos. We observed morula, blastocyst, hatching stages....
Abstract STUDY QUESTION Is there a relation between specific Na+/K+ ATPase isoform expression and localization in human blastocysts the developmental behavior of embryo? SUMMARY ANSWER α1, β1 β3 are main isoforms expressed no association was found level their respective mRNAs rate blastocyst expansion. WHAT IS KNOWN ALREADY In mouse embryos, α1 basolateral membrane trophectoderm (TE) cells believed to be involved blastocoel formation (cavitation). DESIGN, SIZE, DURATION A total 20 surplus...
Abstract Purpose This study investigated the consistency between results of preimplantation genetic testing for aneuploidy performed on trophectoderm (TE) cells and remaining blastocyst cells. Methods TE biopsy was 29 surplus cryopreserved human blastocysts. Biopsy samples blastocysts were processed using VeriSeq PGS kit, chromosomal statuses compared by next‐generation sequencing. Results Discordance observed in status 11 out biopsied Concordance 12 classified as euploid 7 17 aneuploid....
<title>Abstract</title> During human embryonic development, early cleavage-stage embryos are more susceptible to errors. Studies have shown that many problems occur during the first mitosis, such as direct cleavage, chromosome segregation errors, and multinucleation. However, mechanisms whereby these errors mitosis in remain unknown. To clarify this aspect, present study, we imaged discarded living two-pronuclear stage zygotes using fluorescent labeling confocal microscopy without...
Abstract Objective: Pancreatic cancer is one of the highly malignant tumors. Its diagnosis requires invasive ultrasound endoscopic biopsy or retrograde cholangiopancreatography (ERCP). However, these methods are limited by false-positive results and complications. Liquid biopsy, which uses blood to detect KRAS mutations in cancer, can be used overcome hurdles. only about 30% cases detected as a diagnostic marker using plasma from patients with stage I-II pancreatic its clinical application...
Abstract Study question How multinucleation and fragmentation occur in the first mitosis human embryos? Summary answer The mitotic spindles had heterogeneity, shapes movements of were strongly associated with fragmentation. What is known already Studies have shown that many problems during mitosis, such as multinucleation. However, cell biologic mechanisms whereby these errors embryos remain unknown. To clarify this aspect, it necessary to analysis dynamics DNA cytoskeleton using live...
<title>Abstract</title> During human embryonic development, early cleavage divisions are prone to errors, with abnormal division, non-segregation of chromosomes, and multinucleation often observed during the first mitosis. Some studies suggest that nuclear status at 2 cell stage does not affect blastocyst progression or implantation; however, chromosomal aneuploidy is linked 4-cell stage. We fluorescently labelled DNA microtubules in 21 blastomeres 2-cell embryos used confocal microscopy...
Research questionHow does the cost-related oocyte cryopreservation (OoC) vary by facility in Japan, and what data is provided on websites about OoC procedures?DesignWebsite survey. The of all 621 facilities that provide assistive reproductive technology registered Japan were surveyed 2021. Data included rates explicit statements regarding provision for only medical reasons (medical group) or non-medical (non-medical group). Based whether not perform clearly stated cost their websites, we...
Abstract Dynamic morphological changes in the chromosomes and cytoskeleton occur mammals including humans, during early embryonic development, abnormalities such as chromosomal aneuploidy when development does not proceed normally. In previous reports, behavior of DNA mammalian embryos has conventionally been visualized observed by injecting target molecule mRNA, with a fluorescent substance-expressing gene incorporated, into embryos. However, genetic information human embryo to induce...
Abstract Purpose Dynamic morphological changes in the chromosome and cytoskeleton occur mammals humans during early embryonic development, abnormalities such as chromosomal aneuploidy when development does not proceed normally. Visualization of intracellular organelles allows elucidation mammalian embryos. The behavior DNA embryos has conventionally been observed by injecting target molecule mRNAs, incorporating a fluorescent substance‐expressing gene, into In this study, we visualized...
Peritoneal pregnancy is relatively rare and accounts for approximately 1% of all ectopic pregnancies. Accurately diagnosing peritoneal before surgery difficult; thus, gynecologists may encounter the disease accidentally.Many studies have reported that preoperatively diagnosed as tubal pregnancy.
A 74-year-old woman presented with left lateral abdominal pain. Abdominal echography revealed hydronephrosis and a pelvic mass. The patient underwent adnexal resection of suspected ovarian tumor was diagnosed follicular lymphoma (FL) clinical stage IIIA, grade 2. treated rituximab-combined chemotherapy achieved complete remission. most common histological types are diffuse large B-cell Burkitt lymphoma, FL being an extremely rare variant. We herein report case as hydronephrosis.
Abstract Time-lapse incubators have become increasingly popular in assisted reproductive technology, allowing for the observation of developmental process, which may be useful selection human embryos suitable transplantation. Dynamic morphological changes chromosomes and cytoskeleton occur during early embryonic development, including humans, abnormalities such as chromosomal aneuploidy when development does not proceed normally. Chromosome cytoskeletal dynamics are difficult to observe with...
Research question: What are the costs of oocyte cryopreservation (OoC) for fertility preservation website statement in Japan?Design: Website survey. The websites all 621 facilities that provide Assistive Reproductive Technology registered with Japanese Society Obstetrics and Gynecology were surveyed 2021. data collected from rates explicit statements they OoC only medical reasons (medical group) or non-medical (non-medical group). For perform OoC, we compared annual storage cost between...