A5049357499- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Antibiotic Use and Resistance
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial biofilms and quorum sensing
- Nosocomial Infections in ICU
- Enterobacteriaceae and Cronobacter Research
- Mycobacterium research and diagnosis
- Urinary Tract Infections Management
- Pneumonia and Respiratory Infections
- Infection Control in Healthcare
- Infective Endocarditis Diagnosis and Management
- Vibrio bacteria research studies
- Escherichia coli research studies
- Tuberculosis Research and Epidemiology
- Salmonella and Campylobacter epidemiology
- Streptococcal Infections and Treatments
- Medical Device Sterilization and Disinfection
- RNA and protein synthesis mechanisms
- Infectious Diseases and Tuberculosis
- HIV Research and Treatment
- Surgical site infection prevention
- Reproductive tract infections research
- Syphilis Diagnosis and Treatment
Walter Sisulu University
2024
University of Cape Town
2024
Frere Hospital
2024
National Institute for Medical Research
2019-2023
Ralph H. Johnson VA Medical Center
2006-2021
General Department of Preventive Medicine
2021
Medical University of South Carolina
1991-2020
Musgrove Park Hospital
2018
Charleston Area Medical Center
1981-2006
Rutgers, The State University of New Jersey
1997-2002
We have selected mutations in genes encoding components of the signaling pathway for alpha interferon (IFN-alpha) by using a specially constructed cell line. The upstream region IFN-regulated human gene 6-16 was fused to Escherichia coli guanine phosphoribosyltransferase (gpt) and transfected into hypoxanthine-guanine phosphoribosyltransferase-negative cells. These cells express gpt only presence IFN-alpha. They grow medium containing hypoxanthine, aminopterin, thymidine plus IFN are killed...
OBJECTIVE. Healthcare-acquired infections (HAIs) cause substantial patient morbidity and mortality. Items in the environment harbor microorganisms that may contribute to HAIs. Reduction surface bioburden be an effective strategy reduce The inherent biocidal properties of copper surfaces offer a theoretical advantage conventional cleaning, as effect is continuous rather than episodic. We sought determine whether placement alloy-surfaced objects intensive care unit (ICU) reduced risk HAI....
2fTGH is a human cell line containing the selectable marker guanine phosphoribosyltransferase regulated by alpha interferon (IFN-alpha). Two IFN-alpha-unresponsive mutants were isolated previously at low frequency (ca. 10(-8)) selecting mutagenized cells in selective medium 6-thioguanine and IFN-alpha. By using five rounds of mutagenesis, can be an appreciably higher frequency, greater than 3 x 10(-7). Five new have been isolated, all are recessive, as two we described previously. The seven...
Current antibiotic therapies for Clostridium difficile-associated diarrhea have limitations, including progression to severe disease, recurrent C. diarrhea, and selection nosocomial pathogens. Tolevamer, a soluble, high-molecular weight, anionic polymer that binds difficile toxins A B is unique nonantibiotic treatment option.In this 3-arm, multicenter, randomized, double-blind, active-controlled, parallel-design phase II study, patients with mild moderately were randomized receive 3 g of...
The contribution of environmental surface contamination with pathogenic organisms to the development health care-associated infections (HAI) has not been well defined. microbial burden (MB) associated commonly touched surfaces in intensive care units (ICUs) was determined by sampling six objects 16 rooms ICUs three hospitals over 43 months. At month 23, copper-alloy surfaces, inherent antimicrobial properties, were installed onto monitored 8 rooms, and effect that this application had on...
Methicillin-resistant staphylococci are disseminated all over the world and frequent causes of health care- community-associated infections. strains typically carry acquired mecA gene that encodes a low-affinity penicillin-binding protein (PBP), designated PBP2a or
Abstract Antimicrobial resistance results in increased morbidity, mortality, and costs of health care. Prevention the emergence dissemination resistant microorganisms will reduce these adverse effects their attendant costs. Appropriate antimicrobial stewardship that includes optimal selection, dose, duration treatment, as well control antibiotic use, prevent or slow among microorganisms. A comprehensively applied infection program interdict strains
Previously we described human cell line 2fTGH, in which expression of guanine phosphoribosyltransferase is tightly controlled by the upstream region interferon (IFN)-stimulated gene 6-16. After mutagenesis 2fTGH and selection with 6-thioguanine IFN-alpha, isolated 11.1, a recessive mutant that does not respond to IFN-alpha. We now describe U2, second mutant, selected similarly, complements 11.1. U2 had no response IFN-alpha or IFN-beta, its IFN-gamma was partially defective. Although many...
Tefibazumab (Aurexis), a humanized monoclonal antibody that binds to the surface-expressed adhesion protein clumping factor A, is under development as adjunctive therapy for serious Staphylococcus aureus infections. Sixty patients with documented S. bacteremia (SAB) were randomized and received either tefibazumab at 20 mg/kg of body weight single infusion or placebo in addition an antibiotic(s). The primary objective study was determining safety pharmacokinetics. An additional assess...
Methicillin-resistant Staphylococcus aureus (MRSA) is a major pathogen in hospitals. Current antimicrobial regimens for eradicating colonizing strains are not well defined and often complicated by the emergence of resistance. The combination novobiocin plus rifampin vitro vivo was found to prevent resistant populations initially susceptible MRSA, particularly resistance rifampin. We therefore studied, randomized, double-blind, multicenter comparative trial, versus...
From the clinical viewpoint, Enterobacter cloacae and aerogenes are two most important species of genus Enterobacter. E. lacks lysine arginine decarboxylases, whereas has these enzymes. currently accounts for 40%–12% all cases gram-negative bacteremia although it is not a cause at some hospitals. This study showed that been endemic medical center hospital least five years caused 4.5% in 1978. In May 1977 July 1979 outbreaks due to occurred burn ward hospital. Most infected patients had...
<ns4:p><ns4:italic>Staphylococcus aureus </ns4:italic>of the many staphylococcal species is most common cause of both skin and soft tissue infection severe infections including <ns4:italic>Staphylococcus aureus</ns4:italic> bacteremia (SAB). Many antibiotics are active against staphylococci, yet over last 40 years antibiotic resistance, particularly resistance to beta-lactam antibiotics, has plagued antimicrobial therapy. The term “methicillin resistance” a historic now refers ability in...
We have selected mutations in genes encoding components of the signaling pathway for alpha interferon (IFN-alpha) by using a specially constructed cell line. The upstream region IFN-regulated human gene 6-16 was fused to Escherichia coli guanine phosphoribosyltransferase (gpt) and transfected into hypoxanthine-guanine phosphoribosyltransferase-negative cells. These cells express gpt only presence IFN-alpha. They grow medium containing hypoxanthine, aminopterin, thymidine plus IFN are killed...
Abstract The reported prevalence of nasal carriage methicillin-resistant Staphylococcus au reus (MRSA) by hospital personnel averages 2.5%. From August 1985 to September 1987, 155 patients at our Veterans' Affairs Medical Center (VAMC) were colonized or infected with MRSA. In December 1986, only two (2.1%) 94 healthcare workers identified as carriers. Prompted a sharp increase in the number MRSA early contact tracing 450 employees, whom 36 (8%) Thirty-five percent surgical residents (7 20)...
Abstract Background Optimal OPAT dosing of vancomycin (VAN) is challenging in obese patients. Multiple Bayesian pharmacokinetics (PK) models to aid AUC estimation exist, including several obesity-specific models. PK population the absence drug serum levels (a priori) are useful initiate therapy, but once obtained, model performance based upon individual patient posteriori). This study first evaluation optimal for patients receiving OPAT. Methods Analysis included all who received VAN...
Studies were designed to evaluate the effectiveness of pulsed modulated UV light waveforms for killing bacteria. Exposure five strains bacteria information encoded in decreased colony population from a confluent lawn less than 20 colonies. However, approximately 2,000 colonies survived treatment with same intensity and time exposure lacking information.
A new scheme for identification of coagulase-negative staphylococci was applied to 138 consecutive urinary isolates staphylococci. The most common species were Staphylococcus epidermidis (53%), S. hominis (12%), and haemolyticus (10%). saprophyticus comprised only 5%. disk method antibiotic susceptibility all grouped together disclosed resistance commonly penicillin (35%), tetracycline (33%), methicillin (27%), sulfonamide (24%). This pattern also seen specifically with epidermidis. Further...
Journal Article Anti-Staphylococcal Activity of Teicoplanin, Vancomycin, and Other Antimicrobial Agents: The Significance Methicillin Resistance Get access Victor E. Del Bene, Bene Department Medicine, Division Infectious Diseases, Medical University South Carolina, Veterans Administration Center, Charleston, Carolina Please address requests for reprints to Dr. 171 Ashley Avenue, 29425. Search other works by this author on: Oxford Academic PubMed Google Scholar Joseph F. John, Jr., Jr. James...