Romana Iftikhar

ORCID: 0000-0001-7964-3778
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Research Areas
  • Plant Virus Research Studies
  • Plant and Fungal Interactions Research
  • Insect-Plant Interactions and Control
  • Plant Disease Resistance and Genetics
  • Insect Resistance and Genetics
  • Plant-Microbe Interactions and Immunity
  • Legume Nitrogen Fixing Symbiosis
  • Species Distribution and Climate Change
  • Lepidoptera: Biology and Taxonomy
  • Plant and animal studies
  • Photosynthetic Processes and Mechanisms
  • Crop Yield and Soil Fertility
  • Environmental DNA in Biodiversity Studies
  • Transgenic Plants and Applications
  • Microbial Community Ecology and Physiology
  • Animal Virus Infections Studies
  • Dengue and Mosquito Control Research
  • bioluminescence and chemiluminescence research
  • Plant Genetic and Mutation Studies
  • Nematode management and characterization studies
  • Plant Pathogenic Bacteria Studies
  • Genetic and Environmental Crop Studies
  • Plant Pathogens and Resistance
  • Plant pathogens and resistance mechanisms
  • Genomics and Phylogenetic Studies

University of Agriculture Faisalabad
2023-2025

University of Faisalabad
2024

Washington State University
2013-2023

Government College Women University Faisalabad
2017-2020

National Institute for Biotechnology and Genetic Engineering
2013-2016

Pakistan Institute of Engineering and Applied Sciences
2014

Although thrips are globally important crop pests and vectors of viral disease, species identifications difficult because their small size inconspicuous morphological differences. Sequence variation in the mitochondrial COI-5' (DNA barcode) region has proven effective for identification many groups insect pests. We analyzed barcode sequence among 471 from various plant hosts north-central Pakistan. The Barcode Index Number (BIN) system assigned these sequences to 55 BINs, while Automatic Gap...

10.1371/journal.pone.0146014 article EN cc-by PLoS ONE 2016-01-07

The Oleaceae family, encompassing key genera such as Fraxinus (ash trees), Olea (olives), Jasminum (jasmine), Syringa (lilac), and Forsythia, plays a crucial ecological economic role. Despite their importance, the evolutionary dynamics immune system adaptations of NLR (Nucleotide binding leucine-rich repeats) gene family remain largely unexplored. This study employs high-throughput comparative genomics to investigate evolution across family. genus is widely distributed both New Old Worlds,...

10.1186/s12870-025-06233-2 article EN cc-by-nc-nd BMC Plant Biology 2025-02-26

Although Pakistan has rich biodiversity, many groups are poorly known, particularly insects. To address this gap, we employed DNA barcoding to survey its insect diversity. Specimens obtained through diverse collecting methods at 1,858 sites across from 2010-2019 were examined for sequence variation in the 658 bp barcode region of cytochrome c oxidase 1 (COI) gene. Sequences nearly 49,000 specimens assigned 6,590 Barcode Index Numbers (BINs), a proxy species, and most (88%) also possessed...

10.7717/peerj.13267 article EN cc-by PeerJ 2022-04-25

Abstract Dahlia mosaic virus (DMV), an aphid‐transmitted caulimovirus in the family Caulimoviridae , is important pathogen of dahlia ( variabilis ). Development rapid, and sensitive diagnostic tools essential surveillance management DMV. A loop‐mediated isothermal amplification (LAMP) assay was developed for DMV detection. The LAMP targets reverse transcriptase region genome optimal at a temperature 60°C run time 60 min, detected through fluorescence detection by agarose gel electrophoresis....

10.1111/aab.12574 article EN Annals of Applied Biology 2020-01-20

Abstract Onion thrips (Thrips tabaci Lindeman, Thysanoptera: Thripidae) causes severe damage to many horticultural and agronomic crops worldwide. It also acts as a vector of several plant viruses. T. is key pest Allium cepa in the United States. However, there limited information available on genetic variation within between populations States its evolutionary parameters. In current study, 83 specimens were collected from A. 15 different locations comprising four states A total 92 mtCOI gene...

10.1093/jee/toad039 article EN Journal of Economic Entomology 2023-04-13

Whole genome duplication plays a significant role in plant evolution by providing raw materials that can be modified natural or artificial selection. Nucleotide binding site leucine rich repeat receptor (NLRs) like other gene families are clusters of genes created and their size reflects the number duplicated genes. NLR encode immune receptors facilitate identification effector compounds produced pathogen as part triggered immunity (ETI). The accurate characterization substantially...

10.1016/j.stress.2023.100254 article EN cc-by-nc-nd Plant Stress 2023-10-18

Onion (Allium cepa L.) is an important vegetable crop in Pakistan. According to the Food and Agricultural Organization (FAO), Pakistan world's fifth largest onion producer. The area production 127.8 thousand hectares 1.7 million tons, respectively, with a yield of 13.8 tons per hectare during 2012. agro-ecological diversity country enables almost year round. Iris yellow spot virus (IYSV; family Bunyaviridae, genus Tospovirus), transmitted principally by Thrips tabaci, economically viral...

10.1094/pdis-05-13-0502-pdn article EN Plant Disease 2013-06-18

Iris yellow spot, caused by spot orthotospovirus (IYSV) (Genus: Orthotospovirus , Family: Tospoviridae ), is an important disease of Allium spp. The complete N gene sequences 142 IYSV isolates curated sequence data from GenBank were used to determine the genetic diversity and evolutionary pattern. In silico restriction fragment length polymorphism (RFLP) analysis, codon-based maximum likelihood studies, differentiation flow within populations genotypes investigated. Bayesian phylogenetic...

10.3389/fmicb.2021.633710 article EN cc-by Frontiers in Microbiology 2021-09-20

Dahlia common mosaic virus (DCMV) was previously reported to infect only dahlias ( variabilis). However, DCMV identified for the first time in association with Silphium spp. The complete genome of cloned and sequenced from naturally occurring symptomatic silphium plants grown Kansas. two isolates showed over 98% sequence identity those dahlia D. variabilis endogenous pararetroviral (DvEPRS) also detected all 19 samples tested this study, suggesting that DvEPRS may exist as an integrated...

10.1094/phytofr-12-23-0156-sc article EN cc-by-nc-nd PhytoFrontiers™ 2024-03-22

Within the family Fabaceae , genus Glycine is composed of two subgenera annuals (2n=40) and perennials. This life strategy transition may have differentially affected evolution various gene families. Its cultivated species G. max has high level susceptibility to major pathogens including viruses, bacteria fungi. Understanding nucleotide-binding domain leucine-rich repeat ( NLR ) genes in soybean paramount importance due their central role plant immunity potential improving disease resistance...

10.3389/fpls.2024.1383135 article EN cc-by Frontiers in Plant Science 2024-07-09

Whole genome duplication plays a significant role in plant evolution by providing raw materials that can be modified natural or artificial selection. Nucleotide binding site leucine rich repeat receptor (NLRs) like other gene families are clusters of genes created and their size reflects the number duplicated genes. The accurate identification characterization NLR substantially contributes to repertoire resistance improves production. ancestors Fabaceae family have underwent whole (WGD)...

10.2139/ssrn.4510816 preprint EN 2023-01-01

Fall Armyworm, Spodoptera frugiperda, the most notorious insectpest of maize, has led to major crop losses globally. The experiment was conducted study impact three maize varieties on S. frugiperda populations and their assessment. results revealed that variety YH-1898 had highest germination rate, larval infestation, pupal density, number tassels, total grain yield, (84.06±2.14%), (15.41±0.61 larvae/plant), (12.44±0.93 tassels/plant) (9324.71±266.63 kg/acre) standard week-03 week-23,...

10.34016/pjbt.2023.20.02.852 article EN cc-by Pakistan Journal of Biotechnology 2023-12-24
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