A5016309244- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
- DNA Repair Mechanisms
- Lung Cancer Treatments and Mutations
- Esophageal Cancer Research and Treatment
- Genetic factors in colorectal cancer
- Cancer-related Molecular Pathways
- PARP inhibition in cancer therapy
- interferon and immune responses
- Ferroptosis and cancer prognosis
- Immune Cell Function and Interaction
- Advanced Breast Cancer Therapies
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Tea Polyphenols and Effects
- Caveolin-1 and cellular processes
- Pancreatic and Hepatic Oncology Research
- Single-cell and spatial transcriptomics
- Peptidase Inhibition and Analysis
- Esophageal and GI Pathology
- Galectins and Cancer Biology
- Fungal Infections and Studies
- Cancer-related cognitive impairment studies
Shenzhen Second People's Hospital
2022-2024
Shenzhen University Health Science Center
2022-2024
Shenzhen Institutes of Advanced Technology
2024
Chinese Academy of Sciences
2024
Guangzhou Medical University
2023
University of Hong Kong
2018-2022
Chinese University of Hong Kong
2021
Granzyme A (GZMA) is a potential prognostic target for various cancer types. However, its therapeutic significance in breast with immune infiltration remains controversial. We analyzed GZMA expression and value cell infiltration.Data was obtained from patients registered at The Cancer Genome Atlas. correlation performed between patient's clinicopathological features such as age, pathologic stage, metastasis overall survival (OS), disease-specific (DSS), progress free interval (PFI)....
Fanconi anemia patients with germline genetic defects in FANCD2 are highly susceptible to cancers. Esophageal squamous cell carcinoma (ESCC) is a deadly cancer. Little known about the function of ESCC. For detailed molecular and mechanistic insights on functional role ESCC, vivo vitro assays RNA sequencing approaches were used. Utilizing Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) technology, knockout models established examine impact mouse for tumor growth metastasis...
Esophageal squamous cell carcinoma (ESCC) occurs with highest frequency in China over 90% mortality, highlighting the need for early detection and improved treatment strategies. We aimed to identify ESCC cancer predisposition gene(s). Our study included 4,517 individuals. The discovery phase using whole‐exome sequencing (WES) 186 familial patients from high‐risk China. Targeted gene validation of 598 genes 3,289 Henan 1,228 moderate‐risk Hong Kong Chinese. A WES approach identified BRCA2...
Abstract Despite pronounced associations of major histocompatibility complex (MHC) regions with nasopharyngeal carcinoma (NPC), causal variants underlying NPC pathogenesis remain elusive. Our large-scale comprehensive MHC region deep sequencing study 5689 Hong Kong Chinese identifies eight independent NPC-associated signals and provides mechanistic insight for disrupted transcription factor binding, altering target gene transcription. Two novel protective variants, rs2517664 ( T = 4.6%, P...
Background:The Sirtuin (SIRT) family consists of seven evolutionary conserved NAD-dependent deacetylases that play important roles in various cancers, including breast cancer (BC).SIRTs expression has been reported to have prognostic value BC, but these studies used limited sample size and yielded inconsistent conclusions.This study evaluated the association SIRT3 other SIRT members with survival neoadjuvant chemotherapy outcomes.Methods: BC patients' data was obtained from TCGA-BRCA,...
Introduction Cancer-associated fibroblasts (CAFs) are a diverse group of cells that significantly impact the tumor microenvironment and therapeutic responses in breast cancer (BC). Despite their importance, comprehensive profile CAFs BC remains to be fully elucidated. Methods To address this gap, we utilized single-cell RNA sequencing (scRNA-seq) delineate CAF landscape within 14 normal-tumor paired samples. We further corroborated our findings by analyzing several public datasets, thereby...
The patients with dual oesophageal squamous cell carcinoma (ESCC) and hypopharyngeal cancer (HPC) have poor prognosis; their underlying genetic pathogenesis is unclear. We hypothesise that development of synchronous ESCC/HPC depends on multicentricity or independent origin, rather than multifocality due to local lateral spreading.Multiple region whole-exome sequencing (M-WES) clonality analysis were used assess clonal relationship spatial inter- intra-tumour heterogeneity (ITH) in 62 tumour...
Cyclic-dependent kinase (CDK) 4/6 kinases, as the critical drivers of cell cycle, are involved in tumor progression various malignancies. Pharmacologic inhibitors CDK4/6 have shown significant clinical prospects treating hormone receptor-positive and human epidermal growth factor receptor-negative (HR + /HER2-) breast cancer (BC) patients. However, acquired resistance to (CDK4/6i), a common issue, has developed rapidly. It is great significance that identification novel therapeutic targets...
Tripartite motif-containing genes (TRIMs), with a ubiquitin ligase's function, play critical roles in antitumor immunity by activating tumor-specific immune responses and stimulating tumor proliferation, thus affecting patient outcomes. However, the expression pattern prognostic values of TRIMs breast cancer (BC) are not well clarified. In this study, several datasets software were integrated to perform comprehensive analysis investigate their prognosis BC. We found that TRIM59/46...
Tripartite motif-containing protein (TRIM) family members play crucial roles in carcinogenesis and chemotherapy resistance. In this study, we aimed to determine whether TRIM58 expression is related patient responses neoadjuvant therapy (NAT) their survival outcome.Immunohistochemistry was performed on female breast cancer samples from biopsies before NAT Shenzhen Second People's Hospital. Univariate multivariate logistic regression tests were used analyze the association between pathological...
Unbiased whole-exome sequencing approaches in familial esophageal squamous cell carcinoma (ESCC) initially prioritized RAD50 as a candidate cancer predisposition gene. The combined study with 3289 Henan individuals from Northern China identified two pathogenic protein truncation variants, p.Q672X and recurrent p.K722fs variant at the zinc hook domain significantly conferring increased ESCC risk. Effects of ~10-fold higher risk were observed, when compared to East Asians gnomAD database....
Esophageal squamous cell carcinoma (ESCC) is a deadly (SCC) of the esophagus. Development SCCs associated with deregulation lineage program and/or keratinocyte terminal differentiation by genomic and genetic aberrations; thus, these processes must be tightly controlled to maintain normal development. Zinc finger protein 750 (<em>ZNF750</em>) gene involved in frequently mutated putatively silenced ESCC, which implicates its function as potential differentiation‑related suppressor ESCC. The...
Abstract Introduction: Esophageal squamous cell carcinoma (ESCC) has an especially high incidence in Northern China, where there is evidence for a significant familial association. We performed targeted next-generation sequencing (NGS) analysis on ESCC germline samples compared to non-cancer controls from the same high-risk region and compiled list of candidate cancer predisposition genes. Interestingly, genes related Fanconi Anemia (FA) - BRCA pathway are enriched list. Among these FA-BRCA...
Abstract Purpose: The genetic basis of esophageal squamous cell carcinoma (ESCC) still remains unclear. Several genome-wide association studies identified some common variants associated with small effects. Here we aimed to identify rare deleterious in cancer predisposition genes, which may contribute the susceptibility ESCC a large effect, using family history-positive (FH+) samples from Mainland China Henan high-risk region by targeted sequencing. Methods: A two-phase study was conducted,...
Abstract Esophageal squamous cell carcinoma (ESCC), a histological subtype of esophageal cancer, is highly prevalent malignancy in Southeast Asia with poor prognosis and high mortality rate. From previous Next-Generation Sequencing (NGS) studies that sampled familial ESCC cases from Henan, hotspot China, rare germline variants RAD50 homolog, double strand break repair protein (RAD50) were identified predicted to be deleterious by silico models. We hypothesized patients carrying...
Abstract Introduction: Wild-type (WT) Polθ, encoded by POLQ gene, is a specialized DNA polymerase which helps to protect cells against genomic instability allowing double-strand break (DSB) repairs through the alternative end-joining (AltEJ) pathway. In our previous targeted gene sequencing studies, we identified high frequency of germline variants associated with esophageal squamous cell carcinoma (ESCC) in an endemic high-risk region China. Here this study, aim determine functional impact...
Introduction: Esophageal squamous cell carcinoma (ESCC) has a remarkably high incidence in Northern China. Our next-generation sequencing analysis on familial ESCC samples suggests that members of the Fanconi Anemia (FA) pathway play important roles development. anemia complementation group D2 (FANCD2) as key player FA multiple cellular functions. It is well-known for its role DNA damage repair through pathway. Patients with germline FANCD2 mutations are prone to tumor formation. Identifying...
Abstract Introduction: Esophageal squamous cell carcinoma (ESCC) has a remarkably high incidence in Northern China. Our next-generation sequencing analysis on familial ESCC samples suggests that members of the Fanconi Anemia (FA) pathway play important roles development. anemia complementation group D2 (FANCD2) as key player FA multiple cellular functions. It is well-known for its role DNA damage repair through pathway. Patients with germline FANCD2 mutations are prone to tumor formation....
Esophageal cancer occurs with extraordinarily high incidence and dismal survival in high-risk regions of Northern China, highlighting the unmet need for early detection to improve patient survival. Combining enrichment genetic component strategy unbiased whole-exome sequencing (WES) approaches, we performed next-generation (NGS) analysis 186 familial esophageal squamous cell carcinoma (ESCC) cases involving two generations ≥2 family members diagnosed ESCC, including proband. We identified...