A5021704846- Chemokine receptors and signaling
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- DNA Repair Mechanisms
- Glycosylation and Glycoproteins Research
- Enzyme Structure and Function
- Herpesvirus Infections and Treatments
- T-cell and B-cell Immunology
- Receptor Mechanisms and Signaling
- HIV/AIDS drug development and treatment
- Cytomegalovirus and herpesvirus research
- Fungal and yeast genetics research
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Biofuel production and bioconversion
- Genomics and Chromatin Dynamics
- Carcinogens and Genotoxicity Assessment
- vaccines and immunoinformatics approaches
- Bacterial Genetics and Biotechnology
- ATP Synthase and ATPases Research
- Simulation Techniques and Applications
- South Asian Cinema and Culture
- CRISPR and Genetic Engineering
- Genetics and Neurodevelopmental Disorders
- Fermentation and Sensory Analysis
Scripps Research Institute
2006-2021
International AIDS Vaccine Initiative
2017-2021
University of California, San Diego
2014-2020
University of Montana
2015
University of Southern California
2007-2008
University of Colorado Health
2008
Washington University in St. Louis
2003
Molecular “go” signals reveal their secrets Chemokines are proteins that direct how cells move within the body. For instance, chemokines help immune locate invading pathogens and ensure position themselves correctly a developing organ. Cells detect through G protein–coupled receptors on surface; however, molecular details of these interact remain unclear (see Perspective by Standfuss). Qin et al. solved crystal structure chemokine receptor CXCR4 bound to viral vMIP-II. Burg domain CX3CL1....
Significance The chemokine receptor axis plays a critical role in numerous physiological and pathological processes, yet the structural basis of interaction with chemokines is poorly understood. Although community agrees on existence two distinct epitopes for recognition receptors by chemokines, conflicting evidence from mutagenesis studies suggested several possibilities receptor:chemokine complex stoichiometry. We use combination computational, functional, biophysical approaches to show...
Chemokines and their receptors are orchestrators of cell migration in humans. Because dysregulation the receptor-chemokine system leads to inflammation cancer, both chemokines highly sought therapeutic targets. Yet one barriers for targeting is limited understanding structural principles behind recognition selectivity. The existing structures do not include CXC subfamily complexes lack information about receptor distal N-termini, despite importance latter signaling, regulation, bias. Here,...
ABSTRACT Soluble, recombinant native-like envelope glycoprotein (Env) trimers of various human immunodeficiency virus type 1 (HIV-1) genotypes are being developed for structural studies and as vaccine candidates aimed at the induction broadly neutralizing antibodies (bNAbs). The prototypic design is designated SOSIP.664, but many HIV-1 env genes do not yield fully efficiently. One such gene CZA97.012 from a neutralization-resistant (tier 2) clade C virus. As appropriately purified, SOSIP.664...
Abstract Methylglyoxal is associated with a broad spectrum of biological effects, including cytostatic and cytotoxic activities. It detoxified by the glyoxylase system or its reduction to lactaldehyde methylglyoxal reductase. We show that reductase (NADPH‐dependent) encoded GRE2 ( YOL151w ). this activity gene partially purifying enzyme identifying MALDI–TOF proteins in candidate bands on SDS–PAGE gels whose relative intensities correlated specific through three purification steps. The were...
The HIV-1 envelope (Env) is a glycoprotein consisting of trimer heterodimers containing gp120 and gp41 subunits that mediates virus entry major target broadly neutralizing antibodies (bnAbs) developed during infection in some individuals. engagement the to host CD4 protein triggers conformational changes allow its binding co-receptors necessary for establish infection. Native-like Env immunogens representing distinct clades have been proposed improve immunogenicity. In present study, we...
Evaluating the structure-function relationship of viral envelope (Env) evolution and development broadly cross-neutralizing antibodies (bnAbs) in natural infection can inform rational immunogen design. In present study, we examined magnitude specificity autologous neutralizing induced rabbits by a novel HIV-1 clade C Env protein (1PGE-THIVC) vis-à-vis those developed an elite neutralizer from whom env sequence was obtained that used to prepare soluble protein. The 1PGE-THIVC trimer displayed...
Methanothermobacter thermautotrophicus minichromosomal maintenance protein (mtMCM) is a 75 kDa that self-assembles into double hexamer structure. The formed by the N-terminal region of mtMCM has highly charged (overwhelmingly net positive) inner channel. Here we investigate effects point mutations some these residues on biological activities mtMCM. Although all mutants were similar to wild type in folding and complex assembly, found impaired helicase activity. study DNA binding ATPase...
Abstract Chemokines and their receptors are orchestrators of cell migration in humans. Because dysregulation the receptor-chemokine system leads to inflammation cancer, both chemokines highly sought therapeutic targets. Yet one barriers for targeting is limited understanding structural principles behind recognition selectivity. The existing structures do not include CXC subfamily complexes lack information about receptor distal N-termini, despite importance latter signaling, regulation,...
Abstract Evaluating the structure-function relationship of viral envelope (Env) evolution and development broadly cross-neutralizing antibodies (bnAbs) in natural infection can inform rational immunogen design. In present study, we examined magnitude specificity autologous neutralizing induced rabbits by a novel HIV-1 clade C Env protein (1PGE-THIVC) vis-à-vis those developed an elite neutralizer from whom env sequence was obtained that used to prepare soluble protein. The thermostable...
Abstract Chemokines are small signaling proteins that control cell migration through the formation of extracellular concentration gradients. Binding chemokines to chemokine receptors activates intracellular pathways, leading chemotaxis leukocytes during an inflammatory response. and their thereby a crucial part immune system regulate number other physiological functions including development angiogenesis. Most belong large family G-protein coupled ubiquitous in human cells constitute ~40%...
Most chemokine receptors are G Protein‐Coupled Receptors (GPCRs), and best known for their role in controlling cell migration the context of immune system function. They also implicated many diseases particularly inflammatory as well cancer HIV, making them important therapeutic targets. Recent structurally biophysical studies have revealed that recognition interface between chemokines is very large characterized by more complex epitopes than previously believed. In this presentation, our...