A5060458756- Cellular Mechanics and Interactions
- Cell Image Analysis Techniques
- Developmental Biology and Gene Regulation
- Business, Innovation, and Economy
- Sustainable Agricultural Systems Analysis
- 3D Printing in Biomedical Research
- Muscle Physiology and Disorders
- Neurobiology and Insect Physiology Research
- Microtubule and mitosis dynamics
- Bioinformatics and Genomic Networks
- Regional Development and Innovation
- Neurogenetic and Muscular Disorders Research
- Environmental and Cultural Studies in Latin America and Beyond
- Banana Cultivation and Research
- Neurosurgical Procedures and Complications
- Hippo pathway signaling and YAP/TAZ
- Environmental and Ecological Studies
- Agricultural Innovations and Practices
- Advanced Materials and Mechanics
- Advanced Fluorescence Microscopy Techniques
- Vascular Malformations Diagnosis and Treatment
- Medical Imaging and Analysis
- Water Resource Management and Quality
- Plant and soil sciences
- Single-cell and spatial transcriptomics
Instituto de Biomedicina de Sevilla
2015-2024
Universidad de Sevilla
2015-2024
Hospital Universitario Virgen del Rocío
2015-2024
Biomedical Research Networking Center on Neurodegenerative Diseases
2013-2024
Instituto de Salud Carlos III
2024
Instituto Nacional de Investigaciones Agropecuarias
2014-2023
McMaster University
2023
European Space Agency
2022
Advanced Imaging Research (United States)
2022
Statistical Research (United States)
2022
As animals develop, tissue bending contributes to shape the organs into complex three-dimensional structures. However, architecture and packing of curved epithelia remains largely unknown. Here we show by means mathematical modelling that cells in bent can undergo intercalations along apico-basal axis. This phenomenon forces have different neighbours their basal apical surfaces. a consequence, epithelial adopt novel term "scutoid". The detailed analysis diverse tissues confirms generation...
Ataxia telangiectasia is caused by mutations in ATM and represents a paradigm for cancer predisposition neurodegenerative syndromes linked to deficiencies the DNA-damage response. The role of as key regulator signalling following DNA double-strand breaks (DSBs) has been dissected extraordinary detail, but impact this process on DSB repair still remains controversial. Here we develop novel genetic molecular tools modify structure ends demonstrate that indeed required efficient accurate...
Live imaging has revolutionized the analysis of developmental biology over last few years. The ability to track in real time dynamic processes that occur at tissue and cellular levels gives a much clearer view development, allows greater temporal resolution, than is possible with fixed tissue. Drosophila imaginal discs are particularly important model many aspects but their small size location inside larva pupa prevented live techniques from extensively being used study. Here, we introduce...
Tissue morphogenesis is intimately linked to the changes in shape and organisation of individual cells. In curved epithelia, cells can intercalate along their own apicobasal axes, adopting a named 'scutoid' that allows energy minimization tissue. Although several geometric biophysical factors have been associated with this 3D reorganisation, dynamic underlying scutoid formation epithelial packing remain poorly understood. Here, we use live imaging sea star embryo coupled deep learning-based...
A patient with raised intracranial pressure secondary to a dural arteriovenous malformation (AVM) of the posterior fossa is presented. Direct shunting arterial blood into transverse sigmoid sinus caused considerable increase sagittal (SSP) and elevation (ICP). Both ICP SSP returned normal values following obliteration AVM by selective embolization.
Abstract The human Alzheimer’s disease (AD) brain accumulates angiogenic markers but paradoxically, the cerebral microvasculature is reduced around Aß plaques. Here we demonstrate that angiogenesis started near plaques in both AD mouse models and samples. However, endothelial cells express molecular signature of non-productive (NPA) accumulate, plaques, a tip cell marker IB4 reactive vascular anomalies with NOTCH activity. Notably, NPA induction by loss presenilin, whose mutations cause...
ABSTRACT Aims To identify potential image biomarkers of neuromuscular disease by analysing morphological and network‐derived features in skeletal muscle biopsies from a murine model amyotrophic lateral sclerosis (ALS), the SOD1 G93A mouse wild‐type (WT) controls at distinct stages progression. Methods Using NDICIA computational framework, we quantitatively evaluated histological differences between WT mice. The process involved selection subset revealing these differences. A discriminative...
Duchenne muscle dystrophy (DMD) is a genetic disorder characterized by progressive skeletal weakness. Dystrophin deficiency induces instability of the sarcolemma during contraction that leads to necrosis and replacement fibro-adipose tissue. Several therapies have been developed counteract fibrotic process. We report effects nintedanib, tyrosine kinase inhibitor, in mdx murine model DMD. Nintedanib reduced proliferation migration human fibroblasts vitro decreased expression genes such as...
Abstract BiaPy is an open-source library and application that streamlines the use of common deep learning approaches for bioimage analysis. Designed to simplify technical complexities, it offers intuitive interface, zero-code notebooks, Docker integration, catering both users developers. While focused on workflows 2D 3D image data, enhances performance with multi-GPU capabilities, memory optimization, scalability large datasets. Although does not encompass all aspects analysis, such as...
Several previous clinical or preclinical studies using computerized texture analysis of MR Images have demonstrated much more discrimination than visual image by the radiologist. In muscular dystrophy, a discriminating power has been already with various methods magnetic resonance images (MRI-TA). Unfortunately, scale gap exists between spatial resolutions histological and making direct correlation impossible. Furthermore, effect modifications on grey level each pixel is complex cannot be...
Decades of research have not yet fully explained the mechanisms epithelial self-organization and 3D packing. Single-cell analysis large libraries is crucial for understanding assembly function whole tissues. Combining imaging with advanced deep-learning segmentation methods essential enabling this high-content analysis. We introduce CartoCell, a deep-learning-based pipeline that uses small datasets to generate accurate labels hundreds cysts. Our method detects realistic morphology cells...
To assess the significance of sclerotic-appearing cricoid and arytenoid cartilage with computed tomography (CT) in patients laryngeal carcinoma.CT scans obtained 75 carcinoma were prospectively studied; CT studies 50 without retrospectively reviewed.Twenty-four (32%) had sclerotic cartilage. Evaluation pathologic specimens 12 these 24 showed 11 cases two Tumor infiltration was demonstrated six but not seven others. In proof, however, tumor adjacent to perichondrium. proportion similar. The...
Abstract Background Most differentiating cells are arrested in G1-phase of the cell cycle and this proliferative quiescence appears important to allow differentiation programmes be executed. An example occurs Drosophila eye imaginal disc, where all synchronized G1 phase prior making a fate choice either initiate first round photoreceptor or re-enter one terminal mitosis. Results We have analysed mechanism temporally regulated order develop an integrated model regulation. find that...
The proneural genes achaete (ac) and scute(sc) are necessary for the formation of external sensory organs(SOs) Drosophila. ac sc expressed in clusters impart their cells with neural potential. For this potential to be realized, SO precursor cell (SOP) arise within a cluster, sufficient protein must accumulate cluster. Here we describe novel gene, charlatan (chn), which encodes zinc finger transcription factor that facilitates accumulation by forming stimulatory loop ac/sc. We find loss...