A5012412964- Wastewater Treatment and Nitrogen Removal
- Microbial Community Ecology and Physiology
- Ammonia Synthesis and Nitrogen Reduction
- Water Treatment and Disinfection
- Constructed Wetlands for Wastewater Treatment
- Water Systems and Optimization
- Urban Stormwater Management Solutions
- Tracheal and airway disorders
- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
- Biosensors and Analytical Detection
- Microbial Fuel Cells and Bioremediation
Northeastern University
2019-2024
Cooperation between comammox and anammox bacteria for nitrogen removal has been recently reported in laboratory-scale systems, including synthetic community constructs; however, there are no reports of full-scale municipal wastewater treatment systems with such cooperation. Here, we report intrinsic extant kinetics as well genome-resolved characterization a integrated fixed film activated sludge (IFAS) system where co-occur appear to drive loss. Intrinsic batch kinetic assays indicated that...
COVID-19 pandemic-related building restrictions heightened drinking water microbiological safety concerns post-reopening due to the unprecedented nature of commercial closures. Starting with phased reopening (i.e., June 2020), we sampled for 6 months from three buildings reduced usage and four occupied residential households. Samples were analyzed using flow cytometry full-length 16S rRNA gene sequencing along comprehensive chemistry characterization. Prolonged closures resulted in 10-fold...
While the co-existence of comammox
Complete ammonia oxidizing bacteria coexist with canonical and nitrite in a wide range of environments. Whether this is due to competitive or cooperative interactions, result niche separation not yet clear. Understanding the factors driving coexistence nitrifiers critical manage nitrification processes occurring engineered natural ecosystems. In study, microcosm-based experiments were used investigate impact nitrogen source loading on population dynamics drinking water biofilter media....
Leveraging comammox
Leveraging comammox Nitrospira and anammox bacteria for shortcut nitrogen removal can drastically lower the carbon footprint of wastewater treatment facilities by decreasing aeration energy, carbon, alkalinity, tank volume requirements while also potentially reducing nitrous oxide emissions. However, their co-occurrence as dominant nitrifying is rarely reported in full-scale treatment. As a result, there poor understanding how operational parameters, particular dissolved oxygen, impact...
Abstract Building closures related to the coronavirus disease (COVID-19) pandemic resulted in increased water stagnation commercial building plumbing systems that heightened concerns microbiological safety of drinking post re-opening. The exact impact extended periods reduced demand on quality is currently unknown due unprecedented nature widespread closures. We analyzed 420 tap samples over a period six months, starting month phased reopening (i.e., June 2020), from sites at three buildings...
Wastewater surveillance is a proven method for tracking community spread and prevalence of some infectious viral diseases. A primary concentration step often used to enrich particles from wastewater prior subsequent quantification and/or sequencing. Here, we present simple procedure concentrating viruses using bacterial biofilm protein nanofibers known as curli fibers. Through genetic engineering, produced fibers functionalized with single-domain antibodies (also nanobodies) specific the...
ABSTRACT The discovery of the complete ammonia oxidizing (comammox) bacteria overturns traditional two-organism nitrification paradigm which largely underpins design and operation nitrogen removal during wastewater treatment. Quantifying abundance, diversity, activity comammox in treatment systems is important for ensuring a clear understanding biotransformations responsible removal. To this end, we conducted yearlong survey 14 full-scale including mainstream conventional simultaneous...
Abstract Studies have found Ca . Nitrospira nitrosa-like bacteria to be the principal or sole comammox in nitrogen removal systems for wastewater treatment. In contrast, multiple populations of strict ammonia and nitrite oxidizers co-exist similar systems. This apparent lack diversity is surprising could impact feasibility leveraging removal. We used full-length 16S rRNA gene sequencing genome-resolved metagenomics compare population-level (i.e., species) with that nitrifiers full-scale...
Abstract While the co-existence of comammox bacteria with canonical nitrifiers is well documented in diverse ecosystems, there still a dearth knowledge about mechanisms underpinning their interactions. Understanding these interaction important as they may play critical role governing nitrogen biotransformation natural and engineered ecosystems. In this study, we tested ability two environmentally relevant factors (nitrogen source availability) to shape interactions between strict ammonia...
ABSTRACT Cooperation between comammox and anammox bacteria for nitrogen removal has been recently reported in laboratory-scale systems including synthetic community construct; however, there are no reports of full-scale municipal wastewater treatment with such cooperation. Here, we report intrinsic extant kinetics as well genome-resolved characterization a integrated fixed film activated sludge (IFAS) system where co-occur appear to drive loss. Intrinsic batch kinetic assays indicated that...
Abstract Complete ammonia oxidizing bacteria coexist with canonical and nitrite in a wide range of environments. Whether this is due to competitive or cooperative interactions, result niche separation not yet clear. Understanding the factors driving coexistence nitrifiers critical manage nitrification processes occurring engineered natural ecosystems. In study, microcosm-based experiments were used investigate impact nitrogen source loading on population dynamics drinking water biofilter...