A5049049507- Ruminant Nutrition and Digestive Physiology
- Gut microbiota and health
- Microbial Community Ecology and Physiology
- Genetic and phenotypic traits in livestock
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Animal Disease Management and Epidemiology
- School Health and Nursing Education
- Ammonia Synthesis and Nitrogen Reduction
- Reproductive Physiology in Livestock
- Animal Nutrition and Physiology
- Wastewater Treatment and Nitrogen Removal
- Clostridium difficile and Clostridium perfringens research
- Vector-Borne Animal Diseases
- Animal Behavior and Welfare Studies
- Wildlife Ecology and Conservation
- T-cell and B-cell Immunology
- Animal Virus Infections Studies
- Mobile Learning in Education
- Liver Disease Diagnosis and Treatment
- Plant Virus Research Studies
- Species Distribution and Climate Change
- Public Health Policies and Education
- Scientific Computing and Data Management
- Antibiotic Resistance in Bacteria
National Animal Disease Center
2022-2024
Washington University in St. Louis
2024
University of Nebraska–Lincoln
2009-2023
United States Department of Agriculture
2022-2023
Mayo Clinic in Florida
2023
WinnMed
2023
Agricultural Research Service
2022-2023
Iowa State University
2022
Northeastern University
2019-2022
Michigan Technological University
2019
The rumen microbial community in dairy cows plays a critical role efficient milk production. However, there is lack of data comparing the composition bacterial main breeds. This study utilizes 16S rRNA gene sequencing to describe Holstein and Jersey fed same diet by sampling microbiota via cannula (Holstein cows) or esophageal tubing (both cows). After collection sample tubing, particles attached strainer were added ensure representative both liquid solid fraction contents. Alpha diversity...
Rumen microbes play a greater role in host energy acquisition than that of gut-associated monogastric animals. Although genome-enabled advancements are providing access to the vast diversity uncultivated microbes, our understanding variables shaping rumen microbial communities is its infancy. Viruses have been shown impact populations through myriad processes, including cell lysis and reprogramming metabolism. However, little known about processes distribution viruses or how may modulate...
ABSTRACT A multicenter clinical study was conducted to evaluate the performance characteristics of Abbott RealTi m e CT/NG assay, a multiplex real-time PCR for simultaneous detection Chlamydia trachomatis and Neisseria gonorrhoeae . The specimens were collected from total 3,832 male female subjects at 16 geographically diverse sites. Specimens included urine samples, urethral swabs, endocervical self-collected clinician-collected vaginal swabs. tested with automated Aptima Combo 2 assay...
To measure critical order check override rates in VA Puget Sound Health Care System's computerized practitioner entry (CPOE) system and to compare 2006 results a similar 2001 study.Analysis of ordering data gathered by post-hoc logging program. Use Pearson's chi-square contingency table test comparing from this study the earlier study.Factors measured were total number orders, frequency types, overrides type comparisons these with previous results.A 37,040 orders generated 908 (2.5%) checks....
Enrichment of adherent-invasive Escherichia coli (AIEC) has been consistently detected in subsets inflammatory bowel disease (IBD) patients. Although some AIEC strains cause colitis animal models, these studies did not systematically compare with non-AIEC strains, and causal links between are still disputed. Specifically, it remains unclear whether shows enhanced pathogenicity compared to that commensal E. found the same ecological microhabitat if vitro phenotypes used classify as...
Dysfunction of Paneth and goblet cells in the intestine contributes to inflammatory bowel disease (IBD) colitis-associated colorectal cancer (CAC). Here, we report a role for NAD+-dependent histone deacetylase SIRT1 control anti-bacterial defense. Mice with an intestinal specific Sirt1 deficiency (Sirt1int−/−) have more consequent rearrangement gut microbiota. From mechanistic point view, effects on mouse cell maturation are mediated by SIRT1-dependent changes acetylation status SPDEF,...
Abstract Ruminants are critical to global food security as they transform lignocellulosic biomass into high-quality protein products. The rumen microbes ferment feed provide necessary energy and nutrients for the ruminant host. However, we still lack insight metabolic processes encoded by most microbial populations. In this study, implemented metagenomic binning approaches recover 2,809 genomes from cattle, sheep, moose, deer, bison. By clustering based on average nucleotide identity,...
Abstract RNAs stored in the metaphase II-arrested oocyte play important roles successful embryonic development. Their abundance is defined by transcriptional activity during growth and selective degradation of transcripts LH-induced maturation. Our previous studies demonstrated that mRNA increased mature ovulated oocytes collected from obese humans mice therefore may contribute to reduced developmental competence associated with metabolic dysfunction. In current study mouse models...
Spray-dried porcine plasma (SDPP) has been considered as an alternative for in-feed antibiotics to improve pig growth performance; however, the effect of SDPP on gut microbiota is unknown. The objective this study was evaluate effects feeding fecal microbial communities nursery pigs. Ninety-six weaned pigs were assigned 16 pens, which allotted two dietary treatments, including control or + (5% and 2.5% inclusion in phase 1 2, respectively) diet. Fecal samples collected at d 0, 7, 14, 21, 28....
<title>Abstract</title> Weaning in conventional pig production is a stressful event that involves abrupt dietary and environmental changes, the post-weaning period associated with increased incidence of disease antibiotic use. As result, there growing demand for non-antibiotic practices to enhance health during this phase production. Current systems wean piglets at relatively young age, it unclear if age weaning impacts shifts intestinal immune populations, particularly intraepithelial T...
Recent studies have demonstrated RAMP, a complete starter feed, to beneficial effects for animal performance. However, how RAMP may elicit such responses is unknown. To understand if adaptation results in changes the rumen bacterial community that can potentially affect performance, we investigated dynamics of composition corn-adapted and RAMP-adapted cattle.During gradual acclimation communities, compared corn using 16S rRNA gene amplicon sequencing. Significant shifts populations across...
Until Euro-American colonization, Indigenous people used fire to modify eco-cultural systems, developing robust Traditional Ecological Knowledge (TEK). Since 1980, wildfire activity has increased due fire-suppression and climate change. In 2017, in Waterton Lakes National Park, AB, the Kenow burned 19,303 ha, exhibiting extreme behavior. It affected forests Eskerine Complex, a native-grass prairie treated with prescribed burns since 2006 reduce aspen (Populus tremuloides) encroachment linked...
Drinking water contains phylogenetic diverse groups of bacteria, archaea, and eukarya that affect the esthetic quality water, infrastructure, public health. Taxonomic, metabolic, ecological inferences drinking microbiome depend on accuracy, quality, completeness genomes are reconstructed through application genome-resolved metagenomics.
Complete ammonia oxidizing bacteria coexist with canonical and nitrite in a wide range of environments. Whether this is due to competitive or cooperative interactions, result niche separation not yet clear. Understanding the factors driving coexistence nitrifiers critical manage nitrification processes occurring engineered natural ecosystems. In study, microcosm-based experiments were used investigate impact nitrogen source loading on population dynamics drinking water biofilter media....
The objectives of this study were to evaluate the effect dried distillers grains with solubles (DDGS) on ruminal biohydrogenation and duodenal flow fatty acids, effects microbial community using Roche 454 pyro-sequencing. Three crossbred steers (average BW 780 ± 137 kg) fitted cannulae used in a 3-diet, 6-period crossover design. Animals housed individual free stalls fed twice daily at 0700 1900 h. Diets (DM basis) 1) CONTROL, 19.5% corn bran, 20% sorghum silage, 60% brome hay, 0.5% trace...
Salmonella enterica subspecies serovar Heidelberg (Salmonella Heidelberg) has caused several multistate foodborne outbreaks in the United States, largely associated with consumption of poultry. However, a 2015-2017 multidrug-resistant (MDR) outbreak was linked to contact dairy beef calves. Traceback investigations revealed calves infected strains exhibited symptoms disease frequently followed by death from septicemia. To investigate virulence characteristics as pathogen bovine, two variants...
Departments of Medical Technology, Medicine and Health Science, Loma Linda University, Linda, CA 92350
Male chickens grow faster and are more efficient in their feed conversion than females. Due to growth the male birds reach broiler weights about 7 10 days sooner These two factors important growers since represents approximately 70% of production costs. Implantation or injection sex hormones female various farm animals has given varied results. Dinusson et al. (1950) found that treatment heifers with testosterone propionate appeared stimulate but effect was short duraton. Woehling (1951) no...
Evaluating the capabilities of text generation models requires approaches that can capture both syntactic and semantic complexities inherent in language. Traditional evaluation methods often rely on human intervention or static benchmarks, which fail to provide necessary scalability depth needed for rigorous assessment. The novel concept token-based prompt manipulation offers an automated, scalable alternative systematically probes model behavior through controlled token-level variations....
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