A5068507295- Nerve injury and regeneration
- Wound Healing and Treatments
- Advanced Drug Delivery Systems
- Tendon Structure and Treatment
- Nerve Injury and Rehabilitation
- Burn Injury Management and Outcomes
- Electrospun Nanofibers in Biomedical Applications
- Bone fractures and treatments
- Drug Solubulity and Delivery Systems
- Pediatric Pain Management Techniques
- Erythropoietin and Anemia Treatment
- Extracellular vesicles in disease
- Pesticide and Herbicide Environmental Studies
- RNA Interference and Gene Delivery
- Orthopedic Surgery and Rehabilitation
- Insect and Pesticide Research
- Pesticide Exposure and Toxicity
- Antibiotics Pharmacokinetics and Efficacy
- Reconstructive Surgery and Microvascular Techniques
- Muscle activation and electromyography studies
- Dermatologic Treatments and Research
- Cerebral Palsy and Movement Disorders
- Spinal Cord Injury Research
- Family and Patient Care in Intensive Care Units
- Pregnancy and preeclampsia studies
University of Arizona
2023-2025
Center for Orthopaedics
2020-2022
Pennsylvania State University
2019-2022
Penn State Milton S. Hershey Medical Center
2020-2022
University Orthopedics Center
2022
University of Alabama at Birmingham
2019-2021
Nanaji Deshmukh Veterinary Science University
2021
Rajasthan University of Veterinary and Animal Sciences
2021
North Denmark Region
2020
Karnataka Veterinary Animal and Fisheries Sciences University
2014-2019
Abstract Following acute sciatic nerve crush injury (SNCI), inflammation and the improper phagocytic clearance of dying Schwann cells (SCs) has effects on remodeling that lead to morbidity incomplete functional recovery. Therapeutic strategies like use erythropoietin (EPO) for peripheral trauma may serve bring immune cell phagocytotic under control support debris clearance. We evaluated EPO’s effect SNCI found EPO treatment increased myelination index (SFI) bolstered anti-apoptosis...
RNA-binding proteins like human antigen R (HuR) are key regulators in post-transcriptional control of gene expression several pathophysiological conditions. Diabetes adversely affects monocyte/macrophage biology and function. It is not known whether diabetic milieu cellular/exosome-HuR its implications on cardiac inflammation fibrosis. Here, we evaluate vitro vivo effects macrophage cellular/exosome-HuR, alterations intercellular cross talk with fibroblasts, impact remodeling. Human failing...
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Gentamicin induced renal complications are well known in humans and animals. Medicinal properties of Withania somnifera (L.) Dunal, Solanaceae, recognized to improve functions. However, the pharmacological function W. is not completely understood. We sought unravel medicinal therapeutic on gentamicin-induced nephrotoxicity wistar rats. Twenty-four adult male rats evenly divided into four groups evaluate vivo nephroprotective nephrocurative gentamicin nephrotoxic Experimental design as...
Silver nanoparticles (AgNPs) are proved to be potential wound healing materials for normal and burn related wounds due their antibacterial antifungal properties. Although, the properties of AgNPs through green synthesis were less reported. In present study, synthesized incorporated in Pluronic F127 gels as a delivery system evaluate potentiality. Interestingly, activity 0.1% w/w was enhanced considerable extent which could corroborated by increased amount hydroxylproline content (2.39 ± 0.28...
After peripheral nerve trauma, insufficient clearance of phagocytic debris significantly hinders regeneration. Without sufficient myelin clearance, Schwann cells (SCs) undergo increased apoptosis, impairing functional recovery. There is no treatment for crush injury (PNCI). Erythropoietin (EPO) an FDA-approved drug anemia, which may help in the PNCI by transdifferentiating resident SCs into repair (rSCs) and enhancing phagocytosis to facilitate removal cellular debris. For first time, we...
Background: Delayed bone healing is common in orthopaedic clinical care. Agents that alter cell function to enhance would change treatment paradigms. 4-aminopyridine (4-AP) a U.S. Food and Drug Administration (FDA)-approved drug shown improve walking patients with chronic neurological disorders. We recently showed 4-AP’s positive effects the setting of nerve, wound, even combined multi-tissue limb injury. Here, we directly investigated 4-AP on fracture healing, where differentiation...
Abstract Background Traumatic peripheral nerve injury (TPNI) is a major medical problem with no universally accepted pharmacologic treatment. We hypothesized that encapsulation of pro-angiogenic erythropoietin (EPO) in amphiphilic PLGA-PEG block copolymers could serve as local controlled-release drug delivery system to enhance neurovascular regeneration after injury. Methods In this study, we synthesized an EPO-PLGA-PEG copolymer formulation. characterized its physiochemical and release...
Abstract Objective Antibiotics (ABX) are widely used for life-threatening infections and also routine surgical operations. Compelling evidence suggests that ABX-induced alterations of gut microbiota composition, termed dysbiosis, linked with diverse disease states including neurological neurodegenerative conditions. To combat the consequences probiotics (PBX) used. dysbiosis is reported to impair function after spinal cord injury. Traumatic peripheral nerve injury (TPNI) results in profound...
The 6-mercaptopurine (6-MP) is an effective immunosuppressant and anti-cancer drug. However, the usage of 6-MP limited due to its well-known side effects, such as myelotoxicity hepato-renal toxicity. To curtail potential toxic we have used chitosan a natural biodegradable biocompatible polysaccharide synthesize 6-Mercaptopurine-Chitosan Nanoparticles (6-MP-CNPs).The 6-MP-CNPssize, morphology, physicochemical interactions, thermal stability were characterized using Dynamic Light Scattering...
Abstract Peripheral nerve transection is associated with permanent functional deficit even after advanced microsurgical repair. While it difficult to investigate the reasons of poor outcomes repairs in humans, we developed a novel pre-clinical method that allows reliable evaluation regeneration, neural angiogenesis, muscle atrophy, and recovery. Adult male C57BL/6 mice were randomly assigned four different types sciatic transection: Simple Transection ( ST ), & Glue TG Stepwise Sutures...
Traumatic peripheral nerve injury (TPNI) represents a major medical problem that results in loss of motor and sensory function, severe cases, limb paralysis amputation. To date, there are no effective treatments beyond surgery selective cases. In repurposing studies, we found daily systemic administration the FDA-approved drug 4-aminopyridine (4-AP) enhanced functional recovery after acute injury. This study was aimed at constructing novel local delivery system 4-AP using thermogelling...
Abstract Severe thermal skin burns are complicated by inflammation and apoptosis, which delays wound healing contributes to significant morbidity. Diverse treatments demonstrate limited success in mitigating these processes accelerate healing. Agents that alter cell behavior improve would treatment paradigms. We repurposed 4-aminopyridine (4-AP), a drug approved the US FDA for multiple sclerosis, treat severe mice (10-week-old C57BL/6 J male weighing 25 ± 3 g). found 4-AP, early stages of...
This protocol describes isolation methods, culturing conditions, and characterization of human primary cells with high yield viability using rapid enzymatic dissociation skin. Primary keratinocytes, fibroblasts, Schwann are all harvested from the newborn foreskin, which is available following standard care procedures. The removed skin disinfected, subcutaneous fat muscle a scalpel. method consists mechanical separation epidermal dermal layers, followed by additional digestion to obtain...
Glibenclamide (GLB) is a second-generation sulfonylurea oral hypoglycemic agent used in the treatment of noninsulin dependent diabetes mellitus.The use this drug limited due to its poor dissolution and pharmacokinetic profile.In order nullify these drawbacks, GLB thin films were prepared evaluated study influence preparatory conditions on physicochemical properties by using Scanning Electron Microscopy (SEM), Fourier Transform Infra-red Spectroscopy (FTIR) Differential Calorimetric (DSC).The...
<title>Abstract</title> Severe thermal skin burns are complicated by inflammation and apoptosis, which delays wound healing contributes to significant morbidity. Diverse treatments demonstrate limited success with mitigating these processes accelerate healing. Agents that alter cell behavior improve would treatment paradigms. We repurposed 4-aminopyridine (4-AP), a drug approved the US FDA for multiple sclerosis, treat severe burns. found 4-AP, in early stages of burn healing, significantly...
This protocol describes isolation methods, culturing conditions, and characterization of human primary cells with high yield viability using rapid enzymatic dissociation skin. Primary keratinocytes, fibroblasts, Schwann are all harvested from the newborn foreskin, which is available following standard care procedures. The removed skin disinfected, subcutaneous fat muscle a scalpel. method consists mechanical separation epidermal dermal layers, followed by additional digestion to obtain...
The discovery of ways to enhance skin wound healing is great importance due the frequency lesions. We discovered that 4-aminopyridine (4-AP), a potassium channel blocker approved by FDA for improving walking ability in multiple sclerosis, greatly enhances healing. Benefits included faster closure, restoration normal-appearing architecture, and reinnervation. Hair follicle neogenesis within healed wounds was increased, both histologically analysis K15 K17 expression. 4-AP increased levels...
Abstract Background Erythropoietin (EPO) promotes myelination and functional recovery in rodent peripheral nerve injury (PNI). While EPO receptors ( EpoR ) are present Schwann cells, the role of PNI is unknown because lack antagonists or cell‐specific knockout animals. Methods Using Cre‐loxP system, we developed a myelin protein zero (Mpz) promoter‐driven mouse model cell MpzCre‐EpoR flox/flox , Mpz‐EpoR‐KO ). control mice were assigned to sciatic crush followed by treatment. Results...
Peripheral nerve crush injury (PNCI) models are commonly used to study damage and the potential beneficial effects of novel therapeutic strategies. Current PNCI rely on inter-device operator precision limit variation with applied pressure. Although inability accurately quantify pressure may result in reduced reproducibility between animals studies, there is very limited information standardization quantification PNCI. To address this deficit, we constructed a device comprised an Arduino UNO...