A5101492728- Immune cells in cancer
- Immune Response and Inflammation
- Inflammatory Bowel Disease
- Cell Adhesion Molecules Research
- Liver Diseases and Immunity
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- Cancer, Hypoxia, and Metabolism
- Aquaculture Nutrition and Growth
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Caveolin-1 and cellular processes
- Pancreatic function and diabetes
- Neuroinflammation and Neurodegeneration Mechanisms
- Reproductive Health and Contraception
- Edible Oils Quality and Analysis
- Meat and Animal Product Quality
- Receptor Mechanisms and Signaling
- Algal biology and biofuel production
- Helicobacter pylori-related gastroenterology studies
- Glycosylation and Glycoproteins Research
- Histone Deacetylase Inhibitors Research
- Fatty Acid Research and Health
- Chemokine receptors and signaling
- Parkinson's Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
National Institute of Technology Durgapur
2014-2024
Pfizer (India)
2023-2024
Gauhati Medical College and Hospital
2015
Cleveland Clinic Lerner College of Medicine
2010-2012
Case Western Reserve University
2010-2012
Cleveland Clinic
2004-2008
University of South Alabama
2000
Abstract Our laboratory is interested in understanding the regulation of NADPH oxidase activity human monocyte/macrophages. Protein kinase C (PKC) reported to be involved regulating phosphorylation components neutrophils; however, regulatory roles specific isoforms PKC phosphorylating particular have not been determined. In this study calphostin C, an inhibitor for both novel (including PKCδ, -ε, -θ, and -η) conventional PKCα -β), inhibited translocation p47phox, essential component monocyte...
Monocyte chemoattractant protein-1 (MCP-1)-induced monocyte chemotaxis is a major event in inflammatory disease. Our prior studies have demonstrated that MCP-1-dependent requires release of arachidonic acid (AA) by activated cytosolic phospholipase A(2) (cPLA(2)). Here we investigated the involvement AA metabolites chemotaxis. Neither cyclooxygenase nor lipoxygenase pathways were required, whereas pharmacologic inhibitors both cytochrome-P450 (CYP) and soluble epoxide hydrolase (sEH) blocked...
Rationale: The alternative activation of monocytes by interleukin (IL)-13 and IL-4 is a significant component the inflammatory response. consequences in diseases remain to be determined. Objective: In this report, we explored how integrins, receptors important for monocyte migration sites, regulate IL-13–mediated activation. We focused on analysis 2 proteins, which are upregulated during development atherosclerosis, an oxidative enzyme 15-lipoxygenase (15-LO) scavenger receptor CD36. Methods...
Monoamine oxidase A (MAO-A) is a mitochondrial flavoenzyme implicated in the pathogenesis of atherosclerosis and inflammation also many neurological disorders. MAO-A has been reported as potential therapeutic target prostate cancer. However, regulatory mechanisms controlling cytokine-induced expression immune or cancer cells remain to be identified. Here, we show that co-induced with 15-lipoxygenase (15-LO) interleukin 13 (IL-13)-activated primary human monocytes A549 non-small cell lung...
Abstract Phosphorylation of the reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase components p67phox and p47phox accompanies assembly activation this enzyme complex. We have previously reported that human monocytes with opsonized zymosan (ZOP), a potent stimulator NADPH activity, results in phosphorylation p47phox. In study, we investigated regulation phosphorylation. Although protein kinase C (PKC)α has been shown to regulate found inhibition PKCα had no effect on Our...
IL-13 is a Th2 cytokine that promotes alternative activation (M2 polarization) in primary human monocytes. Our studies have characterized the functional receptor complex and downstream signaling events response to stimulation alternatively activated monocytes/macrophages. In this report, we present evidence induces of Src family tyrosine kinase, which required for induction M2 gene expression, including 15-lipoxygenase (15-LO). data show kinase activity regulates IL-13-induced p38 MAPK...
ABSTRACT Zymosan, a mimic of fungal pathogens, and its opsonized form (ZOP) are potent stimulators monocyte NADPH oxidase, resulting in the production O2.–, which is critical for host defense against bacterial pathogens efficient immune responses; however, uncontrolled O2.– may contribute to chronic inflammation tissue injury. Our laboratory has focused on characterizing signal transduction pathways that regulate oxidase activity primary human monocytes. In this study, we examined...
Monocytes/macrophages are versatile cells centrally involved in host defense and immunity. Th1 cytokines induce a classical activation program monocytes/macrophages leading to proinflammatory M1 macrophage phenotype while Th2 IL-4 IL-13 promote monocyte differentiation into an alternatively activated, anti-inflammatory M2 phenotype. Although monoamine oxidase A (MAO-A) is primarily known for its action the nervous system, several recent studies have identified MAO-A as signature marker of...
The T-type Ca2+ channel is an important determinant of electrical activity and influx in rat human pancreatic beta-cells. We have identified sequenced a cDNA encoding alpha1-subunit derived from INS-1, the insulin-secreting cell line. sequence indicates protein composed 2,288 amino acids that shares 96.3% identity to alpha1G, neuronal subunit. transmembrane domains are highly conserved, but isoform contains three distinct regions 10 single acid substitutions other regions. Sequencing genomic...
Previously we demonstrated that human glioblastoma cell lines induce apoptosis in peripheral blood T cells through partial involvement of secreted gangliosides. Here show GBM-derived gangliosides the TNF receptor and activation caspase cascade. Culturing lymphocytes with GBM line derived (10-20μg/ml) increased ROS production as early 18 hrs indicated by uptake dye H2DCFDA while western blotting mitochondrial damage evident cleavage Bid to t-Bid release cytochrome-c into cytosol. Within 48-72...
IL-13 induces profound expression of 15-lipoxygenase (15-LO) in primary human monocytes. Our studies have defined the functional IL-13R complex, association Jaks with receptor components, and tyrosine phosphorylation several Stat molecules response to IL-13. Furthermore, we identified both p38MAPK protein kinase Cδ as critical regulators 15-LO expression. In this study, report an ERK1/2-dependent signaling cascade that regulates IL-13-mediated gene We show rapid phosphorylation/activation...
To study the acceptance level of PPIUCD among women attending GMCH for delivery between January 2011 to December 2014 in relation age, parity and mode their complaints during follow up visit.STUDY DESIGN: Retrospective study.METHOD: In this data admitted labour room postpartum OPD same period were analyzed.RESULTS: Acceptance showed an increasing trend, was more multipara clients undergoing caesarean section.32% acceptors age group 26-30 years.The less than 50% total four years period.The...
MCP-1-induced monocyte chemotaxis is a crucial event in inflammation and atherogenesis. Identifying the important signal transduction pathways that control can unravel potential targets for preventive therapies inflammatory disease conditions. Previous studies have shown focal adhesion kinase Pyk2 plays critical role motility. In this study, we investigated MCP-1-mediated activation of (particularly by phosphorylation Tyr402) primary human peripheral blood monocytes. We showed MCP-1 induces...
Abstract The objective of the study was to assess optimum frying condition fish considering multiple perspectives (retention nutritional quality indices [NQI], reduction preparation time, and improvement health benefit) satisfy consumer‐preferred sensory attributes by controlling most impactful process variables (temperature, oil amount). multi‐criteria decision‐making (MCDM) approach is appropriate handle numerous conflicting criteria objectives. First, an artificial neural network (ANN)...
Among different sources that contribute in the global oxidative stress, vast majority of cellular reactive oxygen species (ROS) originate from mitochondrial compartments.Recently, monoamine oxidases (MAOs) are identified as a prominent source ROS.Monoamine localized outer membrane mitochondria and exist two isoforms, MAO-A MAO-B.MAOs flavoenzymes responsible for deamination biogenic amines during this process, H 2 O aldehydes generated intermediate products.The role oxidase cardiovascular...
Inflammation is a condition through which the body responds to infection or tissue injury. It typically characterized by expression of plethora genes involved in inflammation, that are regulated transcription factors, transcriptional co‑regulators, and chromatin remodeling events. Differential mitotically heritable patterns gene without changes DNA sequence essentially controlled epigenetic regulation. Epigenetic mechanisms, such as histone modifications methylation have profound effect on...