A5101800717- Microbial Metabolic Engineering and Bioproduction
- Microbial Community Ecology and Physiology
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Amino Acid Enzymes and Metabolism
- Polyamine Metabolism and Applications
- Enzyme Structure and Function
- Microbial metabolism and enzyme function
- Legume Nitrogen Fixing Symbiosis
- Algal biology and biofuel production
- Cancer Research and Treatments
- Wastewater Treatment and Nitrogen Removal
- Methane Hydrates and Related Phenomena
- Anaerobic Digestion and Biogas Production
- Plant tissue culture and regeneration
- Protein Hydrolysis and Bioactive Peptides
- Plant nutrient uptake and metabolism
- Electrochemical sensors and biosensors
- Porphyrin Metabolism and Disorders
- Transgenic Plants and Applications
- Protease and Inhibitor Mechanisms
- Education and Work Dynamics
- Protist diversity and phylogeny
- Higher Education and Teaching Methods
Shenzhen Maternity and Child Healthcare Hospital
2024
Guangxi University
2007-2019
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2019
Guangxi Normal University for Nationalities
2019
Guangxi University for Nationalities
2009-2018
Abstract Background Fumarase catalyzes the reversible hydration of fumarate to L -malate and is a key enzyme in tricarboxylic acid (TCA) cycle amino metabolism. also used for industrial production from substrate fumarate. Thermostable high-activity fumarases organisms that inhabit extreme environments may have great potential industry, biotechnology, basic research. The marine environment highly complex considered one main reservoirs microbial diversity on planet. However, most...
A novel serine protease inhibitor (serpin) gene designated as Spi1C was cloned via the sequenced-based screening of a metagenomic library from uncultured marine microorganisms. The had an open reading frame 642 base pairs, and encoded 214-amino acid polypeptide with predicted molecular mass about 28.7 kDa. deduced amino sequence comparison phylogenetic analysis indicated that some partial proteinase I4 serpins were closely related. Functional characterization demonstrated recombinant protein...
Butyl glucoside synthesis using bioenzymatic methods at high temperatures has gained increasing interest. Protein engineering directed evolution of a metagenome-derived β-glucosidase Bgl1D was performed to identify enzymes with improved activity and thermostability. An interesting mutant Bgl1D187 protein containing five amino acid substitutions (S28T, Y37H, D44E, R91G, L115N), showed catalytic efficiency (kcat/Km 561.72 mM-1 s-1) toward ρ-nitrophenyl-β-d-glucopyranoside (ρNPG) that increased...
L-lysine decarboxylase (LDC, EC 4.1.1.18) is a key enzyme in the decarboxylation of to 1,5-pentanediamine and efficiently contributes significance biosynthetic capability. Metagenomic technology shortcut approach used obtain new genes from uncultured microorganisms. In this study, subtropical soil metagenomic library was constructed, putative LDC gene named ldc1E isolated by function-based screening strategy through indication pH change decarboxylation. Amino acid sequence comparison...
Abstract The lipase production of Burkholderia sp. GXU56 was influenced by carbon and nitrogen sources, inorganic salts, initial pH the medium cultivation temperature. maximum 580.52 U/mL reached 5 times level basic in optimum at 8.0, 32 °C, 200 rpm 40–48 h. purified 53.6 fold to homogeneity molecular weight 35 KDa on SDS‐PAGE. temperature were 8.0 40 respectively, it stable range 7–8.5 temperatures below 45 °C. activity strongly inhibited Zn 2+ , Cu Co Fe 3+ ions SDS, while stimulated Li +...
Increase in L-serine production is of interest for industry. Here, we describe a metabolic engineering approach to increase the methylotrophic bacterium. mxaF, gene encoding large subunit methanol dehydrogenase, was cloned from Methylobacterium sp. MB200 through transposon mutagenesis. Deletion mxaF prevented strain grow on methanol, suggesting that involved metabolism. Overexpression resulted fivefold dehydrogenase activity compared wild-type. Resting cell assays showed recombinant...
3-Deoxy-d-arabino-heptulosonate-7-phosphate synthase (DAHPS) is a key rate-limiting enzyme in aromatic amino acid anabolism. A new Iβ-type DAHPS gene (aro1A) was identified metagenomic library from subtropical marine mangrove sediment. The encoded polypeptide composed of 272 acids and had maximum similarity 52.4% to known at the level. Multiple sequence alignment, homologous modeling, molecular docking showed that Aro1A typical (β/α)8 barrel-shaped catalytic structural domain DAHPS. motifs...
Anisic acid, the precursor of a variety food flavors and industrial raw materials, can be bioconversed from anethole which extracted star anise fruits. WGB31 strain with anisic acid molar production rate 10.25% was isolated identified as Burkholderia sp. Three significant influential factors, namely, glucose concentration, initial pH value, medium volume were selected their effects evaluated by Box-Behnken Design (BBD). Regression analysis performed to determine response surface methodology...