A5064547978- Chronic Lymphocytic Leukemia Research
- Fungal and yeast genetics research
- Biofuel production and bioconversion
- Multiple Myeloma Research and Treatments
- Microbial Metabolic Engineering and Bioproduction
- Cancer Genomics and Diagnostics
- Acute Myeloid Leukemia Research
- Lymphoma Diagnosis and Treatment
- Polyamine Metabolism and Applications
- Enzyme Catalysis and Immobilization
- Enzyme-mediated dye degradation
- Fungal Biology and Applications
- Amino Acid Enzymes and Metabolism
- Colorectal Cancer Treatments and Studies
- Genomics and Chromatin Dynamics
- Genetic factors in colorectal cancer
- Fermentation and Sensory Analysis
- Immunodeficiency and Autoimmune Disorders
- Yeasts and Rust Fungi Studies
- Biochemical and biochemical processes
- Monoclonal and Polyclonal Antibodies Research
- PI3K/AKT/mTOR signaling in cancer
- Protein Degradation and Inhibitors
- Biopolymer Synthesis and Applications
- Gene expression and cancer classification
Centro de Investigación del Cáncer
2004-2017
Universidad de Salamanca
1997-2014
Consejo Superior de Investigaciones Científicas
1998-2014
A multistep model has been proposed of disease progression starting in monoclonal gammopathy undetermined significance continuing through multiple myeloma, sometimes with an intermediate entity called smoldering and ending extramedullary disease. To gain further insights into the role transcriptome deregulation transition from a normal plasma cell to clonal cell, indolent malignant we performed gene expression profiling 20 patients significance, 33 high-risk myeloma 41 myeloma. The analysis...
Metastatic dissemination is the most frequent cause of death sporadic colorectal cancer (sCRC) patients. Genomic abnormalities which are potentially characteristic such advanced stages disease complex and so far, they have been poorly described only partially understood. We evaluated molecular heterogeneity sCRC tumors based on simultaneous assessment overall GEP both coding mRNA non-coding RNA genes in primary tumor samples from 23 consecutive patients their paired liver metastases. Liver...
Background Patients with chronic lymphocytic leukemia and 13q deletion as their only FISH abnormality could have a different outcome depending on the number of cells displaying this aberration. Thus, cases high 13q- (13q-H) had both shorter overall survival time to first therapy. The goal study was analyze genetic profile 13q-H patients. Design Methods: A total 102 samples were studied, 32 which served validation cohort five healthy donors. Results Chronic patients higher percentages (>80%)...
The DAO1 gene of Trigonopsis variabilis encoding a D-amino acid oxidase (EC 1.4.3.3) was isolated from genomic clones selected for their specific hybridization to synthetic oligodeoxyribonucleotide probes based on regions the enzyme that have been conserved through evolution. nucleotide sequence predicts protein with similarities human, pig, rabbit, mouse and Fusarium solani oxidases. open reading frame T. interrupted by an intron. Dao1p displays two regions, one in N-terminal section--the...
Multiple myeloma (MM) remains incurable despite the introduction of novel agents, and a relapsing course is observed in most patients. Although development genomic technologies has greatly improved our understanding MM pathogenesis, mechanisms underlying relapse have been less thoroughly investigated. In this study, an integrative analysis DNA copy number, methylation gene expression was conducted matched diagnosis samples from Overall, acquisition abnormalities at much more frequent than...
TET2 is involved in a variety of hematopoietic malignancies, mainly myeloid malignancies. Most mutations have been identified disorders, but some also recently described mature lymphoid neoplasms. In contrast to the large amount data about , are available for gene expression. Moreover, role chronic lymphocytic leukemia (CLL) unknown. This study analyzes both expression and 48 CLL patients. was analyzed by exon arrays quantitative real-time polymerase chain reaction (qRT-PCR). Next-generation...
// María Laura Gutiérrez 1 , Luis Antonio Corchete 2 Eugenia Sarasquete del Mar Abad 3 Oscar Bengoechea Encarna Fermiñán 4 Fernanda Anduaga 5 Sofía Carmen Manuel Iglesias Esteban Angoso Jose Alcazar Jacinto García 5, * Alberto Orfao 1, Muñoz-Bellvís and José Sayagués Cytometry Service-NUCLEUS, Cancer Research Center, IBMCC-CSIC/USAL, Department of Medicine, University Salamanca, Institute Biomedical Networking Centre Consortium-CIBER-CIBERONC, Spain Center Service Hematology, Hospital...
ABSTRACT Transcription of the repressible acid phosphatase gene ( KIPHO5 ) in Kluyveromyces lactis is strongly regulated response to level inorganic phosphate (P i present growth medium. We have begun a study promoter region this order identify sequences involved control expression and design new expression-secretion systems K. . Deletion analysis directed mutagenesis revealed two major identical upstream activating (UAS) CACGTG at positions −430 (UAS1) −192 (UAS2) relative ATG initiation...
Cellulose pulp bleaching is one of the main biotechnological applications fungal laccases due to their capacity degrade lignin from unbleached pulp. For this application, not only a low cost enzyme production, but also higher concentrations than those obtained by its natural producers are needed. Heterologous expression laccase in yeasts an option for producing these enzymes at industrial scale. In work heterologous cglcc1 gene, responsible production basidiomicetous fungus Coriolopsis...
We report here the construction of six deletion mutants and analysis their basic phenotype. Deletion cassettes containing KanMX4 marker module long flanking regions homologous to target locus were constructed for each open reading-frames (ORFs YDL088c, YDL087c, YDL086w, YDL085w, YDL084w YDL082w) located on chromosome IV. Sporulation tetrad heterozygous deletant strains revealed that, in FY1679 genetic background, ORFs YDL087c are essential genes vegetative growth whereas YDL085w YDL082w...
Summary: A secreted phosphate-repressible acid phosphatase from Kluyveromyces lactis has been purified and the N-terminal region an internal peptide have sequenced. Using synthetic oligodeoxyribonucleotides based on sequenced regions, genomic sequence, KIPHO5 , encoding protein isolated. The deduced protein, named KIPho5p, consists of 469 amino acids a molecular mass 52 520 Da (in agreement with data obtained after treatment endoglycosidase H). enzyme shows size heterogeneity, apparent in...
Abstract A genomic clone encoding the Paracoccidioides brasiliensis orotidine monophosphate decarboxylase gene ( PbrURA3 ) was isolated by screening a subgenomic plasmid DNA library of this fungus, using PCR amplification product as probe. Sequence analysis revealed that contains an open reading frame 855 bp with single intron (162 bp), and encodes putative 285 amino acids polypeptide estimated molecular weight 31.1 kDa isoelectric point 6.5. The deduced acid sequence predicted 73.4%...
The DAO1 gene of Trigonopsis variabilis encoding a D-amino acid oxidase (EC 1.4.3.3) was isolated from genomic clones selected for their specific hybridization to synthetic oligodeoxyribonucleotide probes based on regions the enzyme that have been conserved through evolution. nucleotide sequence predicts protein with similarities human, pig, rabbit, mouse and Fusarium solani oxidases. open reading frame T. interrupted by an intron. Dao1p displays two regions, one in N-terminal section—the...