A5068001497- Adipose Tissue and Metabolism
- Angiogenesis and VEGF in Cancer
- Fatty Acid Research and Health
- Cancer, Lipids, and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Retinoids in leukemia and cellular processes
- Antioxidant Activity and Oxidative Stress
- Cancer, Hypoxia, and Metabolism
- HIV-related health complications and treatments
- RNA Interference and Gene Delivery
- Cardiovascular Disease and Adiposity
- Epigenetics and DNA Methylation
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Diet, Metabolism, and Disease
- Regulation of Appetite and Obesity
- Extracellular vesicles in disease
- HIV Research and Treatment
- Lysosomal Storage Disorders Research
- Eicosanoids and Hypertension Pharmacology
- Diet and metabolism studies
- Peroxisome Proliferator-Activated Receptors
- Nitric Oxide and Endothelin Effects
- Endoplasmic Reticulum Stress and Disease
- Atherosclerosis and Cardiovascular Diseases
- MicroRNA in disease regulation
Jagiellonian University
2014-2024
University of British Columbia
2015
University of Vienna
2004
University of Southern Denmark
2004
University of Regensburg
2004
University Hospital Regensburg
2004
MicroRNAs (miRNAs) are a family of small, noncoding RNAs that repress gene expression at the post-transcriptional level. Over 700 miRNAs have been identified in human genome, which 20% to 30% regulate protein-coding genes. Functional vitro studies shown critical for endothelial cell and function. were found atherosclerosis, cardiac hypertrophy, arterial hypertension, coronary artery disease, diabetes, inflammatory diseases. We review current knowledge about role cells with emphasis on...
High fat diet impairs nitric oxide (NO) bioavailability, and induces insulin resistance. The link between NO availability the metabolic adaptation to a high is not well characterized. purpose of this study was investigate effect on metabolism in mice with decreased (eNOS-/-) increased (DDAH overexpressed) bioavailability. eNOS-/- (n = 16), DDAH 24), WT 19) were fed (HFD) for 13 weeks. Body weight, biochemical parameters, adipokines monitored. matrigel vivo model CD31 immunostaining used...
Caloric restriction and n-3 polyunsaturated fatty acid (PUFA) supplementation protect from some of the metabolic complications. The aim this study was to assess influence a low calorie diet with or without PUFA on glucose dependent insulinotropic polypeptide (GIP) output insulin sensitivity markers in obese subjects.Obese, non-diabetic subjects (BMI 30-40 kg/m(2)) aged 25-65 yr. were put (1200-1500 kcal/day) supplemented either 1.8 g/day (DHA/EPA, 5:1) (n = 24) placebo capsules for three...
Glucagon-like peptide 1 receptor agonists (GLP-1RAs) are relatively new pharmacological agents used to normalize glucose level in type 2 diabetes. Recently, GLP-1RAs have been approved for the treatment of obesity reduce body weight non-diabetic patients. The extra-pancre-atic effects GLP-1RAs, as well their molecular mechanism action, still poorly understood. Thus this study was aimed verify hypothesis that action involves mitochondria and administration can improve mitochondrial functions....
Nutrient excess enhances glucose-dependent insulinotropic polypeptide (GIP) secretion, which may in turn contribute to the development of liver steatosis. We hypothesized that elevated GIP levels obesity affect markers injury through microRNAs. The study involved 128 subjects (body mass index (BMI) 25-40). Fasting and postprandial GIP, glucose, insulin, lipids, as well fasting alanine aminotransferase (ALT), γ-glutamyltransferase (GGT), cytokeratin-18, fibroblast growth factor (FGF)-19,...
Abstract Background Epigenetics can contribute to lipid disorders in obesity. The DNA methylation pattern be the cause or consequence of high blood lipids. aim study was investigate profile peripheral leukocytes associated with elevated LDL-cholesterol level overweight and obese individuals. Methods To identify differentially methylated genes, genome-wide microarray analysis performed individuals (LDL-CH, ≥ 3.4 mmol/L) versus control LDL-CH, < mmol/L. Biochemical tests such as serum...
Abstract. Objectives : Angiogenesis, the process of formation blood vessels, is essential for many physiological as well pathological processes. It has been shown that human adipose tissue contains a population non‐characterized cells, called stromal‐vascular fraction (SVF) which are able to differentiate into several lineages. The aim this study was determine conditions promoting differentiation progenitors towards endothelial show SVF cells cooperate with differentiated endothelium in...
Recently, the number of new psychoactive substances has significantly increased. Despite systematic introduction prohibition in trade medicinal products which mimic effects illegal drugs, problem concerning this group drugs is still important although knowledge about mechanism action those types scarce. This study aimed to follow neurotoxic effect N-benzylpiperazine (BZP), central nervous system psychostimulant, using human cancer LN-18 cell model. The statistically significant elevation LDH...
Obesity and its complications lead to vascular injury, atherosclerosis, diabetes pathological angiogenesis. One of the models study obesity entanglements is New Zealand Obese mice model. Aim this was check effect high fat diet on changes in biochemical parameters as well process angiogenesis NZO mice. were fed with standard (ST) or (HF) for seven weeks. Body weight serum monitored. The PECAM1 positive vessel-like structures immunostaining, gene expression matrigel penetrating cells by...
To evaluate the effect of insulin resistance in obesity on expression whole blood mRNA and miRNA affecting bone homeostasis as well to estimate influence oral glucose load (OGTT) serum osteocalcin concentration obese individuals with without resistance.Cross-sectional study.Carboxylated (cOC), undercarboxylated (ucOC) total were measured by ELISA subjects (n = 41) (control group) during OGTT. Analysis gene (microarray) miRNAs (real-time PCR) was performed venous (representating samples)...
Abstract Monocyte chemoattractant protein‐1‐induced protein 1 (MCPIP1) has a multidomain structure, which assures its pleiotropic activity. The physiological functions of this include repression inflammatory processes and the prevention immune disorders. influence MCPIP1 on cell cycle cancer cells not been sufficiently elucidated. A previous study by our group reported that overexpression affects viability, inhibits activation phosphoinositide‐3 kinase/mammalian target rapamycin signalling...
Heterozygous familial hypercholesterolemia (heFH) is an autosomal dominant lipid metabolism disorder. Its prevalence 1:250–1:300 people in the population. Patients with heFH have up to 13-fold increased risk of premature coronary artery disease (CAD). If left untreated, men and women typically develop early CAD before ages 55 60, respectively. There evidence that calcification (CAC) aortic valve (AoVC) are more prevalent FH patients than general It documented CAC AoVC predictors...
The expression of apoptosis genes in a commercial pre-designed low-density array from Applied Biosystems was evaluated two human brain cancer cell models, LN-18 and Daoy (HTB-186™) comparison to the reference primary endothelial cells under basic conditions. Analysis gene lines compared normal control revealed features reflecting anti-apoptotic inflammatory characteristics former. There an overall downregulation apoptosis-stimulating both lines, along with upregulation certain inhibitors. A...