A5108007367- Reproductive Biology and Fertility
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Kruppel-like factors research
- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- RNA Interference and Gene Delivery
- Autophagy in Disease and Therapy
- Ovarian cancer diagnosis and treatment
- Pregnancy and preeclampsia studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Chromatin Dynamics
- Animal Genetics and Reproduction
- Computational Drug Discovery Methods
- Phytoestrogen effects and research
- Cancer-related molecular mechanisms research
- Gastrointestinal Tumor Research and Treatment
- Renal and related cancers
- Digestive system and related health
- Autoimmune and Inflammatory Disorders Research
- COVID-19 Clinical Research Studies
- Inflammasome and immune disorders
- RNA regulation and disease
- Metastasis and carcinoma case studies
- Natural Compounds in Disease Treatment
Shanghai Jiao Tong University
2017-2023
Renji Hospital
2022
Hubei Cancer Hospital
2021
International Peace Maternity & Child Health Hospital
2018-2019
Ningxia Medical University
2019
Xiamen University
2015
The etiology and pathogenesis of pre-eclampsia (PE) is unclear, there no ideal early clinical biomarker for prediction PE. competing endogenous RNA (ceRNA) hypothesis a new approach to uncover the molecular pathology first aim this study was perform messenger RNA, long non-coding circular (circRNA) expression profiling human normal severe (SPE) placentas. circRNA, which has stable structure, more suitable than other types RNA. Therefore, second our select some differentially expressed...
Abstract Background Autophagy is required for oogenesis and plays a critical role in response to aging caused by oxidative stress. However, there have been no reports on regulation of cytoprotective autophagy female germline stem cells (FGSCs) Results We found that Spermidine (SPD) significantly increased protein expression markers microtubule-associated 1 light chain 3 beta-II (MAP1LC3B-II/LC3B-II) sequestosome-1/p62 (SQSTM1/p62), evoked autophagic flux FGSCs. Moreover, SPD the number...
Abstract The male’s ability to reproduce is completely dependent on Sertoli cells. However, the mechanisms governing functional integrity of cells have remained largely unexplored. Here, we demonstrate that deletion Shp2 in results infertility mice. In knockout mice (SCSKO), a normal population was observed, but blood-testis barrier (BTB) not formed. ablation initiated untimely and excessive differentiation spermatogonial stem (SSCs) by disturbing expression paracrine factors. As...
MicroRNAs (miRs) are functionally important in spermatogenesis, which is the self‐renewal or differentiation of spermatogonial stem cells (SSCs). Here, we report a novel role for miR‐10b regulating mouse SSCs. We showed that was highly expressed SSCs vitro and enhanced SSC proliferation. Knockdown significantly increased apoptosis compared with controls. Kruppel‐like factor 4 found to be target gene enhancement These findings further our understanding provide basis diagnosis, treatment,...
Several studies have recently revealed the regulatory mechanisms underlying female germline stem cell (FGSC) differentiation, proliferation, and apoptosis, but other biological processes such as autophagy its mechanism in FGSCs are largely unclear. The use of small chemical compounds may be a good approach to further investigate process FGSC development. In this study, we used ZCL-082, derivative benzoxaboroles, treat FGSCs. Using counting kit-8 (CCK8) 5-ethynyl-2′-deoxyuridine (EdU) assays,...
Postnatal female germline stem cells (FGSCs) are a type of cell with self-renewal ability and the capacity differentiation toward oocyte. The proliferation, differentiation, apoptosis FGSCs have been researched in recent years, but autophagy has not explored. This study investigated effects small-molecule compound 89 (C89) on underlying molecular mechanism vitro. Cytometry, Cell Counting Kit-8 (CCK8), 5-ethynyl-2’-deoxyuridine (EdU) assay showed that number, viability, proliferation were...
During male meiosis, the Y chromosome can form perfect pairing with X chromosome. However, it is unclear whether mammalian Female germline stem cells (FGSCs) without a transdifferentiate into functional haploid spermatid-like (SLCs).We found that spermatogenesis was restarted by transplanting FGSCs Kitw/wv mutant testes. Complete meiosis and formation of SLCs induced in vitro testicular mice, cytokines retinoic acid. Healthy offspring were produced sperm derived from vivo...
This study aimed to clarify the regulation and mechanism of meiotic initiation in FGSC development.FGSCs were induced differentiate into meiosis differentiation medium. RNA sequencing was performed analysis difference transcription level. High-through chromosome conformation capture (Hi-C) changes three-dimensional chromatin structure. Chromosome further confirmed a spatial loop. ChIP-qPCR dual luciferase reporter used test interaction between Stimulated by retinoic acid gene 8 (STRA8)...
This data descriptor is an extended version of the methodologies which have been described in a related paper [1]; its purpose to disseminate raw and analyzed produced this experiment. For more insight please see research article, "competing endogenous RNA expression profiling pre-eclampsia identifies hsa_circ_0036877 as potential novel blood biomarker for early pre-eclampsia" (Xiaopeng Hu Junping Ao, 2018) [1]. Using microarray analysis, we investigated competing (ceRNA) profiles placentas...
Abstract We previously identified the mouse dynein axonemal intermediate chain 2 ( Dnaic2 ) gene. This gene expresses a component of complex that functions in cilia or flagella. found overexpression results female subfertility and male infertility. In this study, we generated knockdown KD mice potential regulatory mechanisms involved function. For phenotype analysis, body weight was lighter size smaller than wild‐type mice. A total 45% these were infertile due to sperm abnormalities males,...
The key structure of the interface between spike protein Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) and human angiotensin-converting enzyme (hACE2) acts as an essential switch for cell entry by virus drugs targets. However, this is largely unknown. Here, we tested three peptides receptor binding domain (RBD) found that peptide 391–465 aa major hACE2-interacting sites in SARS-CoV-2 RBD. We then identified amino acid residues (403R, 449Y, 454R) were critical interaction RBD...
The xenoestrogens nonylphenols (NPs), which are materials used in the plastic polymer industry, considered endocrine disruptors a wide range of organisms. Studies have shown that human health problems, such as infertility and reproductive toxicology, linked with NPs. However, mechanism by NPs interfere male reproduction is not fully elucidated. Here, we found 4-NP can result impairment reduce androgen receptor (AR) protein levels rat sertoli cells vitro vivo . Moreover, performed RNA...