A5101881391- Immune cells in cancer
- Phagocytosis and Immune Regulation
- Eicosanoids and Hypertension Pharmacology
- Chemokine receptors and signaling
- Liver Disease Diagnosis and Treatment
- Hepatocellular Carcinoma Treatment and Prognosis
- Mitochondrial Function and Pathology
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Organ Transplantation Techniques and Outcomes
- Endoplasmic Reticulum Stress and Disease
- Cancer Mechanisms and Therapy
- Cancer Immunotherapy and Biomarkers
- Drug Transport and Resistance Mechanisms
- Glycosylation and Glycoproteins Research
- Animal testing and alternatives
- Nanoplatforms for cancer theranostics
- Ferroptosis and cancer prognosis
- Liver Disease and Transplantation
- Cancer Research and Treatments
- Kruppel-like factors research
- Neuroinflammation and Neurodegeneration Mechanisms
- Protein Hydrolysis and Bioactive Peptides
- Biopolymer Synthesis and Applications
- Liver physiology and pathology
First Affiliated Hospital Zhejiang University
2019-2024
Hangzhou First People's Hospital
2021-2023
Zhejiang University
2020-2023
Westlake University
2023
National Health and Family Planning Commission
2021
HCC is a malignant disease. Compared with tyrosine kinase inhibitors (the classical therapy), immune checkpoint are more effective in the treatment of HCC, despite their limited efficacy. Among these restricted factors, exhaustion tumor-infiltrated lymphocytes, especially CD8 + T cells, core event. We aimed to determine key factors contributing T-cell infiltration and investigate underlying mechanisms.
Abstract Objectives The discrepancy between supply and demand of organ has led to an increased utilization steatotic liver for transplantation (LT). Hepatic steatosis, however, is a major risk factor graft failure due susceptibility ischaemia‐reperfusion (I/R) injury during transplantation. Materials methods To assess the plasticity phenotype immune cells within microenvironment at single‐cell level, RNA‐sequencing (scRNA‐Seq) was carried out on 23 675 from transplanted rat livers....
Ferroptosis, which is driven by iron-dependent lipid peroxidation, plays an essential role in liver ischemia-reperfusion injury (IRI) during transplantation (LT). Gp78, E3 ligase, has been implicated metabolism and inflammation. However, its IRI ferroptosis remains unknown. Here, hepatocyte-specific gp78 knockout (HKO) or overexpressed (OE) mice were generated to examine the effect of on IRI, a multi-omics approach (transcriptomics, proteomics, metabolomics) was performed explore potential...
Abstract Hepatic steatosis significantly elevates the vulnerability of graft to ischemia-reperfusion (I/R) injury during liver transplantation (LT). We investigated protective role insulin-induced gene 2 (Insig2) in steatotic liver’s I/R and underlying mechanisms. Employing mouse model with Insig2 knock-out or hepatocyte-specific overexpression high-fat diets induce steatosis, we subjected these mice hepatic injury. The primary hepatocytes isolated from were used vitro hypoxia/reoxygenation...
Cytokeratin 19-positive (CK19+) hepatocellular carcinoma (HCC) is an aggressive subtype characterized by early recurrence and chemotherapy tolerance. However, there no specific therapeutic option for CK19+ HCC. The correlation between tumor expression status of CK19 were studied in 206 patients undergoing liver transplantation CK19-/+ HCC cells isolated to screen effective antitumor drugs. effects regorafenib evaluated patient-derived xenograft (PDX) models from 10 patients. mechanism on was...
The efficacy of immune checkpoint inhibitors in the treatment hepatocellular carcinoma (HCC) remains limited, highlighting need for further investigation into mechanisms underlying resistance. Accumulating evidence indicates that tumor-associated macrophages (TAM) within tumor microenvironment demonstrate a key role evasion and This study explored TAMs HCC microenvironment. Our findings reveal expressing CX3C motif chemokine receptor 1 (CX3CR1) induced T-cell exhaustion through IL27...
Patient-derived xenograft (PDX) models provide a promising preclinical platform for hepatocellular carcinoma (HCC). However, the molecular features associated with successful engraftment of PDX have not been revealed.HCC tumor samples from 76 patients were implanted in immunodeficient mice. The expression was evaluated by immunohistochemistry. Patient and characteristics as well expressions compared using Chi-square test. independent prediction parameters identified logistic regression...
Hepatic ischemia-reperfusion (IR) injury is the primary reason for complications following hepatectomy and liver transplantation (LT). Insulin-induced gene 2 (Insig2) one of several proteins that anchor reticulum in cytoplasm essential metabolism inflammatory responses. However, its function IR remains ambiguous.Insig2 global knock-out (KO) mice with adeno-associated-virus8 (AAV8)-delivered Insig2 hepatocyte-specific overexpression were subjected to a 70% hepatic model. Liver was assessed by...
BACKGROUND: Precise recipient selection optimizes the prognosis of liver transplantation (LT) for hepatocellular carcinoma (HCC). Alpha-fetoprotein (AFP) is most commonly used biomarker diagnosis and HCC in clinical context. As a crucial molecule methionine cycle, homocysteine (Hcy) level has been proved to be related progression metastasis. OBJECTIVE: We aimed explore prognostic capacity pre-transplant serum Hcy LT HCC. METHODS: This study retrospectively enrolled 161 patients who had...
<p>Supplementary Figure S4. The effect of different immunotherapy combinations on tumor infiltrating NK/NKT cells</p>
<p>Supplementary Figure S3. The effect of CX3CR1 neutralizing antibody on macrophages</p>
<p>Supplementary Fig S6. PGE2 released by tumor cells under immune attack promotes CX3CR1 upregulation on macrophages</p>
<p>Supplementary Fig S5. In vitro co-culture experiments of TAM and CD8<sup>+</sup> T cells the effect CX3CR1 overexpression on BMDMs</p>
<p>Table S1</p>
<p>Table S3</p>
<p>Supplementary Figure S1. Flow cytometry and FACS gating strategy</p>
<div>Abstract<p>The efficacy of immune checkpoint inhibitors in the treatment hepatocellular carcinoma (HCC) remains limited, highlighting need for further investigation into mechanisms underlying resistance. Accumulating evidence indicates that tumor-associated macrophages (TAM) within tumor microenvironment demonstrate a key role evasion and This study explored TAMs HCC microenvironment. Our findings reveal expressing CX3C motif chemokine receptor 1 (CX3CR1) induced T-cell...
<p>Supplementary Figure S2. H22 orthotopic HCC immunotherapy model</p>
<p>Supplementary Fig S6. PGE2 released by tumor cells under immune attack promotes CX3CR1 upregulation on macrophages</p>
<p>Table S2</p>