A5058372989- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Virus-based gene therapy research
- Nanoplatforms for cancer theranostics
- Immunotherapy and Immune Responses
- Extracellular vesicles in disease
- Graphene and Nanomaterials Applications
- Adenosine and Purinergic Signaling
- MicroRNA in disease regulation
- Nanopore and Nanochannel Transport Studies
- Endoplasmic Reticulum Stress and Disease
- Bone Tissue Engineering Materials
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- CAR-T cell therapy research
- Liver Disease Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Natural Compounds in Disease Treatment
- Mitochondrial Function and Pathology
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Mechanisms and Therapy
- Immune cells in cancer
- Photodynamic Therapy Research Studies
Hangzhou First People's Hospital
2022-2025
Westlake University
2024-2025
Zhejiang Chinese Medical University
2023-2025
Hangzhou Normal University
2025
Zhejiang University
2015-2023
National Center for Nanoscience and Technology
2020-2023
Zhejiang Shuren University
2023
University of North Carolina at Chapel Hill
2019-2021
Communities In Schools of Orange County
2021
State Key Laboratory of Chemical Engineering
2015-2017
Selective gene expression in tumors via responsive dissociation of polyplexes triggered by intracellular signals is demonstrated. An esterase-responsive charge-reversal polymer mediates selective the cancer cells high esterases over fibroblasts low esterase activity. Its therapy with TRAIL suicide effectively induces apoptosis HeLa but does not activate to secrete WNT16B, enabling potent few side effects.
Anti-programmed cell death-1 (PD-1)/programmed death-ligand 1 (PD-L1) antibodies are currently used in the clinic to interupt PD-1/PD-L1 immune checkpoint, which reverses T dysfunction/exhaustion and shows success treating cancer. Here, we report a histone demethylase inhibitor, 5-carboxy-8-hydroxyquinoline (IOX1), inhibits tumour Jumonji domain-containing 1A (JMJD1A) thus downregulates its downstream β-catenin subsequent PD-L1, providing an antibody-independent paradigm interrupting...
Abstract Interleukin 12 (IL12) is a potent pro‐inflammatory chemokine with multifunction, including promoting cytotoxic T‐cell‐mediated killing of cancer cells. IL12‐based gene therapy can overcome IL12's life‐threatening adverse effects, but its clinical translation has been limited by the lack systemic gene‐delivery vectors capable efficiently transfecting tumors to produce sufficient local IL12. Macrophages inherently excrete IL12, and tumor‐associated macrophages (TAMs) are major tumor...
Ionizable cationic lipids are recognized as an essential component of lipid nanoparticles (LNPs) for messenger RNA (mRNA) delivery but can be confounded by low lipoplex stability with mRNA during storage and in vivo delivery. Herein, the rational design combinatorial synthesis esterase-triggered decationizable quaternium lipid-like molecules (lipidoids) reported to develop new LNPs high efficiency improved stability. This top lipidoid carries positive charges at physiological condition...
Manganese ions (Mn2+) are an immune activator that enhances the activation of both cGAS and STING proteins. The signaling subsequential responses predominantly associated with endoplasmic reticulum (ER). Therefore, ER targeting Mn2+ in subcellular compartments would promote pathways. Herein, we report design ER-targeted manganese-based nanocomplexes (NCs) by complexation a zwitterionic polymer, poly[2-(N-oxide-N,N-dimethylamino) ethyl methacrylate] (OPDMA). Mn/OPDMA (Mn/OPDMA NCs) keep long...
Gene therapy has demonstrated effectiveness in many genetic diseases, as evidenced by recent clinical applications. Viral vectors have been extensively tested gene-therapy trials, but nonviral such cationic polymers or lipids are much less used due to their lower gene-transfection efficiencies. However, the advantages of vectors, easily tailored structures, nonimmunogenetics, and relatively low cost, still drive great efforts improve transfection A reverse question asks if with current gene...
Desmoplastic tumors are normally resistant to nanoparticle-based chemotherapy due dense stroma and limited particle permeability inside the tumor. Herein, we reported that hydralazine (HDZ)—an antihypertension vasodilator—would dramatically promote nanoparticle penetration in advanced desmoplastic tumors. First, a HDZ–liposome system was developed for tumor-selective delivery of HDZ. After three injections HDZ–liposomes at dose 15 mg/kg, tumor remarkably reduced, along with ameliorated...
Adipocytes are the primary cellular components within tumor microenvironment (TME) of triple-negative breast cancer (TNBC). Increasing evidence suggests that tumor-associated adipocytes (TAAs) can aggravate progression, exacerbate immunosuppressive TME and compromise therapeutic efficacy. In this study, biological effect TAAs is first investigated, C-C Motif Chemokine Ligand 2 (CCL2) which mainly secreted by in extracellular environment identified as key mediator. CCL2 recruits immune cells...
Abstract Cationic polymers dynamically complex DNA into complexes (polyplexes). So, upon dilution, polyplexes easily dissociate and lose transfection ability, limiting their in vivo systemic gene delivery. Herein, it is found that polyplex's stability endocytosis pathway determine its dose‐dependence. The of hydrophilic polycations have dose‐dependent integrity lysosome‐trafficking endocytosis; at low doses, most these dissociate, the remaining few are internalized trapped lysosomes,...
Pancreatic ductal adenocarcinoma (PDAC), one of the worst prognosis types tumors, is characterized by dense extracellular matrix, which compresses tumor vessels and forms a physical barrier to inhibit therapeutic drug penetration efficacy. Herein, losartan, an antihypertension agent, applied as stroma modulator developed into nanosystem. A series lipophilic losartan prodrugs are constructed esterification hydroxyl group on fatty acids. Based self-assembly ability hydrodynamic diameter,...
Ferroptosis has garnered attention as a potential approach to fight against cancer, which is characterized by the iron-driven buildup of lipid peroxidation. However, robust defense mechanisms intracellular ferroptosis pose significant challenges its effective induction. In this paper, an gene delivery vehicle was developed transport solute carrier family 7 member 11 (SLC7A11) shRNA (shSLC7A11), downregulates expression channel protein SLC7A11 and glutathione peroxidase 4 (GPX4), evoking...