A5011853666- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Landslides and related hazards
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- RNA Research and Splicing
- SARS-CoV-2 and COVID-19 Research
- Soil and Unsaturated Flow
- CRISPR and Genetic Engineering
- Chemical Synthesis and Analysis
- Chemical Reaction Mechanisms
- Civil and Geotechnical Engineering Research
- Hydrology and Sediment Transport Processes
- Advanced Measurement and Metrology Techniques
- COVID-19 Clinical Research Studies
- Hydraulic flow and structures
- Geomechanics and Mining Engineering
- Industrial Vision Systems and Defect Detection
- Image Processing Techniques and Applications
- Biopolymer Synthesis and Applications
- Organic and Inorganic Chemical Reactions
- Gut microbiota and health
- Manufacturing Process and Optimization
- Ferroptosis and cancer prognosis
Guangzhou Medical University
2025
Jinan University
2024
Huazhong University of Science and Technology
2023-2024
Sichuan University
2009-2024
Guangxi Medical University
2024
Zhejiang University
2024
The Ohio State University
1978-2023
Southern Medical University
2021-2022
State Key Laboratory of Hydraulics and Mountain River Engineering
2018
Zhaoqing University
2013
The increasing prevalence of SARS-CoV-2 variants with spike mutations has raised concerns owing to higher transmission rates, disease severity, and escape from neutralizing antibodies. Rapid accurate detection provides crucial information concerning the outbreaks possible lines transmission. This is vital for infection prevention control. We used a Cas12a-based RT-PCR combined CRISPR on-site rapid system (RT-CORDS) platform detect key in variants, such as 69/70 deletion, N501Y, D614G....
Abstract The most widely used method for intracellular RNA fluorescence labeling is MS2 labeling, which generally relies on the use of multiple protein labels targeted to (MS2) hairpin structures installed interest (ROI). While effective and conveniently applied in cell biology labs, add significant mass bound RNA, potentially impacts steric accessibility native biology. We have previously demonstrated that internal, genetically encoded, uridine-rich internal loops (URILs) comprised four...
We report herein the synthesis and DNA/RNA binding properties of bPNA+, a new variant bifacial peptide nucleic acid (bPNA) that binds oligo T/U acids to form triplex hybrids. By virtue bivalent side chain on similar DNA affinity hybrid thermostability can be obtained with half molecular footprint previously reported bPNA. Lysine derivatives bearing two melamine bases (K
Summary The Bacilius thuringiensis insecticidal crystal protein CryllA has both high mosquito activity and gypsy moth activity; in contrast CryllB, which is 87% homologous, displays no a threefold lower activity. regions responsible for specificity against ( Lymantria dispar ) Aedes aegypti larvae were located by introducing Mlul Xhol sites into homologous positions within the putative domain ii of cryllA cryllB genes, divided almost equally respective second domains three regions. Taking...
We report herein a new class of synthetic reagents for targeting the element nuclear expression (ENE) in MALAT1, long noncoding RNA upregulated many cancers. The cis-acting ENE contains U-rich internal loop (URIL) that forms an 11 base UAU-rich triplex stem with truncated 3′ oligo-A tail protecting terminus from exonuclease digestion and greatly extending transcript lifetime. Bifacial peptide nucleic acids (bPNAs) similarly bind URILs via triple formation between two uracil bases base,...
While the resident microbiome of tumors has been shown to be associated with occurrence and progression non-small cell lung cancer, there remains a significant knowledge gap in understanding correlation between microbial spectrum immunity response cancer therapy. In case adenocarcinoma (LUAD), tumor microenvironment, encompassing diverse array microbes immune cells, plays crucial role modulating therapeutic response. Towards comprehending underlying mechanism, we present microbe-immunity...
Herein, we demonstrate context-dependent molecular recognition of DNA by synthetic bPNA iron and copper complexes, using oxidative backbone cleavage as a chemical readout for binding. Oligoethylenimine bPNAs displaying iron·EDTA or copper·phenanthroline sites were found to be efficient nucleases designed native structured DNAs with T-rich single-stranded domains. Cleavage reactivity depends strongly on structural context, strikingly demonstrated substrates the form (GGGTTA)n. This repeat...
A flexible riverbed protection called tetrahedron framed permeable weirs (TFPW) is proposed to protect riverbeds in mountainous areas from scouring. Under clear water conditions, a series of laboratory flume experiments were performed study the effects TFPW with different layout types on stability riverbeds. The objectives this paper advance understanding role that play erosion process river beds and optimize design for reducing velocity, promoting sediment deposition good structural...
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), a new coronavirus causing Disease 2019 (COVID-19), is major topic of global human health concern. The Delta and Omicron variants have caused alarming responses worldwide due to their high transmission rates number mutations. During one-year follow-up (from June 2020 2021), we included 114 patients with SARS-CoV-2 infection study the long-term dynamics correlative factors neutralizing antibodies (NAbs) in convalescent patients....
We hypothesize that programmable hybridization to noncanonical nucleic acid motifs may be achieved by macromolecular display of binders individual pairs (NCPs). As each recognition element individually have weak binding an NCP, we developed a semi-rational approach detect low affinity interactions between selected nitrogenous bases and sites in duplex DNA RNA. A set fluorogenic probes was synthesized coupling abiotic (triazines, pyrimidines) native RNA thiazole orange (TO) dye. This probe...
A drainage channel is an efficient debris flow countermeasure, but defects in practical function exist due to the non-peer exchange between post-geological hazard investigation and dynamics estimation. Especially a with contracted entrance, structural design depends on peak discharge, velocity of flow, interaction sediment structure. This paper mainly explores flows interacting before part through flume model tests. Results are illustrated as follows: i) In after section, overflow silting...
ABSTRACT Tyrolean weir can be used as an effective solution to address floatation and sediment deposition in runoff hydropower stations. To improve the efficiency accuracy of calculating this structure's water intake capacity. The integrated learning algorithm random forest (RF), firefly (FA), exponential distribution (EDO) are utilized develop that for Cd (qw)i/(qw)T prediction models. Sobol's method SHAP theory introduced analyze above parameters quantitatively qualitatively. It is shown...