A5004827804- RNA Research and Splicing
- Black Holes and Theoretical Physics
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Cosmology and Gravitation Theories
- Advanced biosensing and bioanalysis techniques
- Nuclear Structure and Function
- Particle physics theoretical and experimental studies
- Chromosomal and Genetic Variations
- Spectroscopy and Quantum Chemical Studies
- Advanced Chemical Physics Studies
- DNA and Nucleic Acid Chemistry
- Advanced Fluorescence Microscopy Techniques
- Photochemistry and Electron Transfer Studies
- Paraoxonase enzyme and polymorphisms
- Homotopy and Cohomology in Algebraic Topology
- Galaxies: Formation, Evolution, Phenomena
- Microtubule and mitosis dynamics
- Pulsars and Gravitational Waves Research
- Tryptophan and brain disorders
- Gene expression and cancer classification
- Energy Efficient Wireless Sensor Networks
- Diet and metabolism studies
- Genetics, Bioinformatics, and Biomedical Research
National Health Research Institutes
2013-2025
The Ohio State University Wexner Medical Center
2022-2024
The Ohio State University
2022-2023
Kavli Institute for the Physics and Mathematics of the Universe
2014-2019
The University of Tokyo
2014-2019
Texas A&M University
2007-2011
National Tsing Hua University
2000-2001
In contrast to the well-studied condensation and folding of chromosomes during mitosis, their dynamics interphase are less understood. We deployed a CRISPR-based DNA imaging system track genomic loci situated kilobases megabases apart on single chromosome. Two distinct modes were resolved: local movements as well ones that might reflect translational entire domain within nucleoplasmic space. The magnitude both these increased from early late G1, whereas reduced in S phase. fluctuations...
Abstract Background Vortioxetine, a newer serotonergic antidepressant, has been used to treat adult depressive patients. It reported enhance cognitive functions in While the precise mechanism of action remains unknown, it’ s believed share similarities with selective serotonin reuptake inhibitors (SSRIs). Since previous studies have indicated that SSRIs could alter gut microbial composition, and considering correlation between severity function microbiota through gut-brain axis, we aimed...
Dynamic chromatin organization instantly influences DNA accessibility through modulating local macromolecular density and interactions, driving changes in transcription activities. Chromatin dynamics have been reported to be locally confined but contribute coherent motion across the entire nucleus. However, regulation of dynamics, nuclear orientation compaction subregions along a single chromosome are not well-understood. We used CRISPR-based real-time single-particle tracking polymer models...
We construct non-minimal GUT local models in the F-theory configuration. The gauge group on bulk GS is one rank higher than group. line bundles curves are nontrivial to break down groups. demonstrate examples of SU(5) from GS=SU(6) and GS=SO(10), flipped SO(10) GS=SO(12) GS=E6. obtain complete matter spectra couplings, with minimum exotic contents. breaking MSSM achievable by instanton configurations.
Abstract The most widely used method for intracellular RNA fluorescence labeling is MS2 labeling, which generally relies on the use of multiple protein labels targeted to (MS2) hairpin structures installed interest (ROI). While effective and conveniently applied in cell biology labs, add significant mass bound RNA, potentially impacts steric accessibility native biology. We have previously demonstrated that internal, genetically encoded, uridine-rich internal loops (URILs) comprised four...
BICEP2's detection on the primordial B-mode of CMB polarization suggests that inflation occurred around GUT scale, with tensor-to-scalar ratio r≃0.2. Inspired by this discosvery, we study topological which was driven a double/single/no well potential. We show proper choice parameters, all these three types inflationary models could be consistent constraints from current observations.
A nonstatistical behavior has been observed in the photodissociation of two acyl cyanide compounds, R-C(O)-CN, where R = methyl and tert-butyl groups. The initial activation was achieved by femtosecond two-photon excitation followed ultrafast internal conversion. excited-state dissociation dynamics real time monitoring temporal evolution CN (X) products. product appearance constants are picosecond scales markedly similar for compounds studied. Our analyses revealed that rate does not...
The photodissociation of two acyl cyanide compounds, R–C(O)–CN, where R=methyl and tert-butyl groups, has been investigated using femtosecond time-resolved laser-induced fluorescence (LIF) spectroscopy. Both compounds were excited by two-photon excitation at a total energy ∼6.4 eV the formation free CN(X) radical products was probed in real time monitoring CN X→B LIF signal. results revealed that temporal evolution can be well characterized delayed biexponential rise functions with constants...
Correct chromosome organization in the cell nucleus is essential for genome function. However, dynamics and regulations of large-scale chromosomal conformations beyond single compartments at larger than ten megabases vivo cells remain largely unknown. Here we use CRISPR-Sirius, a high-resolution high-sensitivity real-time imaging technique, to directly visualize distinctions between live osteosarcoma (OS) osteoblasts, suggesting extensive chromatin reorganization during transformation. A...
<h3>Objectives</h3> The aim of this study was to investigate the health hazards in workers exposed nanoparticles during manufacturing and application nanomaterials. <h3>Methods</h3> For 4-year longitudinal study, we recruited 283 nanomaterial-handling 213 non-exposed control from 15 plants Taiwan. Follow-up measurements were done at 6, 12, 24, 36, 48 months. Among them, 206 140 unexposed followed up for more than twice. each participant, a self-administered questionnaire distributed collect...
SUMMARY In contrast to the well-studied condensation and folding of chromosomes during mitosis, their dynamics in interphase are less understood. We developed a sensitive, multicolor system, CRISPR-Sirius, allowing real-time tracking chromosomal loci. tracked loci kilobases megabases apart found significant variation inter-locus distances each pair, indicating differing degrees DNA contortion. resolved two distinct modes loci: saltatory local movements as well translational domain. The...
ABSTRACT We introduce herein a new strategy for intracellular RNA and DNA tracking that is robust, orthogonal complementary to existing methods: F luorogenic U - R ich I nternal L oop (FLURIL) tagging with cell-permeable fluorophore-labeled bifacial Peptide Nucleic Acids (fbPNAs). Our approach uses an 8-nt (U 4 xU ) U-rich internal loop (URIL) in the of interest (ROI) as compact labeling site fluorogenic triplex hybridization bPNA probe (~1 kD). FLURIL thus replaces bp duplex stem labeled...
<h3>Objectives</h3> The market of nanomaterials products is rapidly expanding. lack scientific evidence describing accompanying exposure and cardiovascular health risks regarding its guidance regulation. objectives this study was to examine (1) the association between different biomarkers (2) effect <i>PON1 Q192R</i> genetic polymorphism on among nanoworkers. <h3>Methods</h3> In cross-sectional study, we recruited 258 workers exposed 200 non-exposed controls from 14 nanomaterial handling...